In vivo electrochemical detection of 5-hydroxyindoles in rat cerebral cortex and spinal cord: Differential effects of p-chloroamphetamine, probenecid and clorgyline

Ory-Lavollee, L.; Pointis, D.; Lamour, Y.; Chiang, Chen Yu; Rivot, J.P.

doi:10.1016/0304-3940(84)90491-9

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…The enhanced 300 mV oxidation peak height in the cerebral cortex following the administration of the 5-HT-releasing agent p-CA is consistent with that observed by Ory-Lavollee et al (1984). These authors have also shown that the effect ofp-CA on the 300 mV peak is abolished in rats with depleted endogenous stores of 5-HT, thus supporting the notion that p-CA is a 5-HT-releasing agent.…”

Section: Discussionsupporting

confidence: 86%

“…On the one hand, the selective MAO-A inhibitor clorgyline did not alter the amplitude of the 300 mV peak in the cortex, but it did produce a significant, though modest, attenuation of the signal in the dentate gyrus. Using this MA0 inhibitor, a significant attenuation of the 300 rnV peak was reported to occur in the occipital (Cespuglio et al, 1984) but not in the somatosensory cortex (Ory-Lavollee et al, 1984). This further emphasizes the notion of the regional differences in the functional properties of the 5-HT system.…”

Section: Discussionmentioning

confidence: 83%

“…Our results are therefore in agreement with the anatomical data of the group of Molliver who demonstrated the p-CA, as well as MDA and MDMA, produce a selective destruction of dorsal but not median raphe 5-HT terminals. Furthermore, it has been previously demonstrated that p-CA fails to modify the 300 mV peak recorded from the dorsal horn of the spinal cord (Ory-Lavollee et al, 1984) that is not innervated by the dorsal raphe but by other raphe nuclei, some of which have been shown to be insensitive to p-CA (Fritschy et al, 1987). The precise mode of action of these amphetamine derivatives has not been fully elucidated, but it is known that their cytotoxic effect on dorsal raphe 5-HT terminals can be prevented by the prior administration of a 5-HT reuptake blocker (Berger et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

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Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT₁ receptor agonists and p‐chloroamphetamine

Blier

Serrano

Scatton

1990

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118

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The dorsal and median raphe 5-HT neurons give rise to projections that differ in axon morphology and in vulnerability to certain amphetamine derivatives. The present study was undertaken to determine if these two 5-HT systems possess different functional properties. To this end, we studied the effects of selective 5-HT1A or 5-HT1A/5-HT1B receptor agonists and of p-chloroamphetamine on extracellular levels of indoleamines, as measured by differential pulse voltammetry with extracellular levels of indoleamines, as measured by differential pulse voltammetry with electrochemically pretreated carbon fiber electrodes, in cell body and nerve terminal regions of these subsets of 5-HT neurons in the rat brain. The selective 5-HT1A agonist 8-OH-DPAT produced a gradual decrease in the height of the 300 mV oxidation peak in the dorsal raphe and in the frontal cortex, reaching a maximum of 60% 3 h after the i.v. injection of 30 micrograms/kg. However, the same dose of 8-OH-DPAT was ineffective in the median raphe and in the dentate gyrus that receives its 5-HT innervation exclusively from the median raphe. A higher dose of 8-OH-DPAT (150 micrograms/kg, i.v.) produced a 60% decrease in the height of the 300 mV oxidation peak in the median raphe, whereas only a 20% decrease was obtained in the dentate gyrus. In contrast, the non-selective 5-HT1 agonist RU 24,969 (10 mg/kg, i.p.) caused a 70% reduction of the 300 mV peak height in both the dorsal and median raphe and a 50% decrease in both the frontal cortex and the dentate gyrus. Moreover, although a high dose of 8-OH-DPAT (150 micrograms/kg, i.v.) given alone reduced by 20% the amplitude of the oxidative peak in the dentate gyrus, subsequent administration of RU 24,969 (10 mg/kg, i.p.) caused a further 30% diminution of the oxidative peak height. The greater responsiveness of dorsal as compared to median raphe 5-HT systems to 5-HT1A receptor agonists was confirmed in two further series of experiments. First, the microiontophoretic application of 8-OH-DPAT directly onto 5-HT neurons was three times more potent in suppressing the firing rate of dorsal raphe 5-HT neurons than that of their median raphe congeners. Second, 8-OH-DPAT and buspirone were ten and four times, respectively, more potent in decreasing 5-HT synthesis in the frontal cortex than in the hippocampus.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT₁ receptor agonists and p‐chloroamphetamine

Blier

Serrano

Scatton

1990

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118

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“…Steranka, 1978 ;Fuller, 1992) . This observation is in keeping with other electrochemical data in the hippocampus (Joseph and Kennett, 1981) or cortex (Ory-Lavoll6e et al ., 1984), and, in addition, peak 3 is slightly decreased (present study) or unchanged (Ory-Lavollée et al ., 1984) in the spinal cord with nonselective electrodes (CFE or rnCFE) . Furthermore, pretreatment with allopurinol did not impair the increase in the voltammograms observed with N-mCFEs following PCA administration, with this increase being comparable, both in time course and amplitude, to that observed without allopurinol pretreatment.…”

Section: Discussionsupporting

confidence: 50%

In Vivo Electrochemical Monitoring of Serotonin in Spinal Dorsal Horn with Nafion‐Coated Multi‐Carbon Fiber Electrodes

Rivot

Cespuglio

Puig

et al. 1995

Journal of Neurochemistry

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Biosensors sensitive for in vivo monitoring of serotonin (5-HT) in the CNS by differential normal pulse voltammetry were constructed by coating treated multicarbon fiber electrodes (mCFEs) with Nafion (N-mCFE) . In vitro sensitivities of mCFE and N-mCFE were compared in solutions ranging from 5 nM to 20 pM of uric acid (UA), 5-hydroxyindoleacetic acid (5-HIAA), and 5-HT . The mCFEs were three to seven times less sensitive for 5-HIAA or UA than for 5-HT . Nafion treatment dramatically decreased sensitivity for 5-HIAA and UA of N-mCFEs (-10 3 times), whereas it remained in the nanomolar range for 5-HT . In vivo, in the dorsal horn of the lumbar spinal cord of anesthetized rats, the monoamine oxidase inhibitor clorgyline (10 mg/kg i .p .) produced a reduction (55 ± 3% at 180 min) of peak 3 of oxidation current (characteristic of 5-hydroxyindoles) monitored with mCFEs, but with N-mCFEs (in this latter case the peak was termed 3N) peak 3N increased to 135 ± 5% at 180 min. The 5-HT release-inducer p-chloroamphetamine (PCA; 6 mg/kg i.p .) induced a slight (12 -3% at 150 min) decrease in peak 3 measured with mCFEs, whereas with N-mCFEs PCA induced a rapid increase of peak 3N (137 ± 6% at 90 min) . The xanthine oxidase inhibitor allopurinol (10 mg/kg i.p .) produced a decrease (30 -3% at 180 min) in peak 3 (mCFEs), but peak 3N (N-mCFEs) was not affected (106% at 180 min) . After pretreatment with allopurinol, PCA also produced an increase (135±-6% at 90 min) in peak 3N . These in vitro and in vivo data provide evidence for a highly preferential detection of 5-HT versus 5-HIAA and UA by N-mCFEs, which could be used to follow the extracellular 5-HT concentration within very discrete structures throughout the CNS . Key Words: Carbon fiber-Nafion-Serotonin-Spinal cord -Voltammetry .

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State‐of‐the‐art microelectrodes for in vivo voltammetry

Broderick

1990

Electroanalysis

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A comprehensive view of in vivo electrochemistry (voltammetry) is presented with a detailed evaluation of electrodes and waveforms. Special attention is given to specific kinds of electrode fabrication. The article is presented in an historical format to emphasize both the progression of the electrochemical technology and the inherent stability of the technique. Future prospects relevant to voltammetry are discussed, and 189 references are included.

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In vivo electrochemical detection of 5-hydroxyindoles in rat cerebral cortex and spinal cord: Differential effects of p-chloroamphetamine, probenecid and clorgyline

Cited by 4 publications

References 23 publications

Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT₁ receptor agonists and p‐chloroamphetamine

Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT₁ receptor agonists and p‐chloroamphetamine

In Vivo Electrochemical Monitoring of Serotonin in Spinal Dorsal Horn with Nafion‐Coated Multi‐Carbon Fiber Electrodes

State‐of‐the‐art microelectrodes for in vivo voltammetry

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In vivo electrochemical detection of 5-hydroxyindoles in rat cerebral cortex and spinal cord: Differential effects of p-chloroamphetamine, probenecid and clorgyline

Cited by 4 publications

References 23 publications

Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT1 receptor agonists and p‐chloroamphetamine

Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT1 receptor agonists and p‐chloroamphetamine

In Vivo Electrochemical Monitoring of Serotonin in Spinal Dorsal Horn with Nafion‐Coated Multi‐Carbon Fiber Electrodes

State‐of‐the‐art microelectrodes for in vivo voltammetry

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Product

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Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT₁ receptor agonists and p‐chloroamphetamine

Differential responsiveness of the rat dorsal and median raphe 5‐HT systems to 5‐HT₁ receptor agonists and p‐chloroamphetamine