In vivo evaluation of an electrospun and 3D printed cellular delivery device for dermal wound healing

Clohessy, Ryan M.; Cohen, David J.; Karolina, Stumbraite; Boyan, Barbara D.; Schwartz, Zvi

doi:10.1002/jbm.b.34587

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…To accelerate wound healing, a resorbable fibrous wound dressing that can deliver a cellular payload was developed based on a sacrificial 3D‐printed structure. [ 132 ] To achieve this, an electrospun composite with an inner reservoir was produced using 3D‐printed sacrificial components. The confined empty space created by removing the sacrificial structures generated space for cells and other payloads to be incorporated.…”

Section: Development Of Biomimetic Structures and Tissue‐engineering ...mentioning

confidence: 99%

Manufacturing 3D Biomimetic Tissue: A Strategy Involving the Integration of Electrospun Nanofibers with a 3D‐Printed Framework for Enhanced Tissue Regeneration

Randhawa,

Dutta,

Ganguly

et al. 2024

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Abstract3D printing and electrospinning are versatile techniques employed to produce 3D structures, such as scaffolds and ultrathin fibers, facilitating the creation of a cellular microenvironment in vitro. These two approaches operate on distinct working principles and utilize different polymeric materials to generate the desired structure. This review provides an extensive overview of these techniques and their potential roles in biomedical applications. Despite their potential role in fabricating complex structures, each technique has its own limitations. Electrospun fibers may have ambiguous geometry, while 3D‐printed constructs may exhibit poor resolution with limited mechanical complexity. Consequently, the integration of electrospinning and 3D‐printing methods may be explored to maximize the benefits and overcome the individual limitations of these techniques. This review highlights recent advancements in combined techniques for generating structures with controlled porosities on the micro–nano scale, leading to improved mechanical structural integrity. Collectively, these techniques also allow the fabrication of nature‐inspired structures, contributing to a paradigm shift in research and technology. Finally, the review concludes by examining the advantages, disadvantages, and future outlooks of existing technologies in addressing challenges and exploring potential opportunities.

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Section: Development Of Biomimetic Structures and Tissue‐engineering ...mentioning

confidence: 99%

Manufacturing 3D Biomimetic Tissue: A Strategy Involving the Integration of Electrospun Nanofibers with a 3D‐Printed Framework for Enhanced Tissue Regeneration

Randhawa,

Dutta,

Ganguly

et al. 2024

Small

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“…[ 61 ] When 3D‐printed sacrificial elements were incorporated in the layer‐by‐layer structure, an interconnected void within the electrospun fibers can be formed after removing the sacrificial templates. [ 66 ] Complicated scaffolds with user‐specific patterned structures were also fabricated with controllable cell‐laden multi‐scale sheets by alternate use of 3D printing and electrospinning (Figure 4b). [ 67 ]…”

Section: Methods For Combinationmentioning

confidence: 99%

“…In efforts to further heal these wounds, a resorbable electrospun wound dressing was developed based on the 3D‐printed sacrificial element to incorporate into the wound with the capability of delivering a cellular payload. [ 66 ] The sacrificial elements offered an internal void space, by which an injectable payload can be delivered to the wound site. The in vivo tests confirm that the scaffold can serve as a substrate for reepithelialization and facilitate blood vessel formation in the typical wound model.…”

Section: Applicationsmentioning

confidence: 99%

“…• Only suitable for fabricating planar and tubular-shaped products Tracheal repair, [58][59][60] nerve repair, [55,56,156] vascular grafts, [57,120,121] bone repair [171] Alternate use of 3D printing and electrospinning Vascular grafts, [122,123,[135][136][137] tracheal repair, [109,112] skin repair, [66] cartilage repair, [29,180,183] tympanic membrane scaffold [34] Electrospun fibers as inks of 3D printing…”

Section: Yesmentioning

confidence: 99%

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Combination of 3D Printing and Electrospinning Techniques for Biofabrication

Fazal

Wang

Radacsi

2022

Adv Materials Technologies

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This review presents the different combination methods of 3D printing and electrospinning processes and discusses the advantages of each method. Also, the applications of products of the hybrid process in the biomedical field, including tissue engineering and regenerative medicine, are thoroughly discussed. 3D printing and electrospinning are separate approaches for generating structured materials in a variety of biomedical applications. However, some shortfalls, such as the low resolution of 3D printing and uncontrollable shape of electrospun products, limits their application performances. Therefore, new ideas that combine 3D printing and electrospinning methods are proposed to give full play to their strengths and mitigate their weaknesses, which provide an effective and powerful platform for fabricating novel structural materials. Finally, a future vision regarding challenges and perspectives in the combination of 3D printing and electrospinning techniques is proposed.

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“…The liquid/polymeric solution is sprayed through the nozzle in the presence of high electrical forces. This cost-effective approach offers several significant advantages of altering the processing parameters to regulate the resultant particles, such as the distance between the collector and nozzle and the applied voltage ( Wang et al, 2018 ; Clohessy et al, 2020 ; Miller et al, 2021 ). In a case, Mozari et al initially generated 3D spheroids of MSCs, which were then encapsulated using the electrospray technique in the micro-scale alginate beads and subsequently into the injectable thermosensitive PNIPAAm-based hydrogel matrices ( Figure 6A ) ( Nilforoushzadeh et al, 2020 ).…”

Section: Microfabrication Strategiesmentioning

confidence: 99%

Polymeric microcarriers for minimally-invasive cell delivery

Duan

Yu²,

Hu³

et al. 2023

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims at restoring tissue defects by applying the three-dimensional (3D) biomimetic pre-formed scaffolds to restore, maintain, and enhance tissue growth. Broadly speaking, this approach has created a potential impact in anticipating organ-building, which could reduce the need for organ replacement therapy. However, the implantation of such cell-laden biomimetic constructs based on substantial open surgeries often results in severe inflammatory reactions at the incision site, leading to the generation of a harsh adverse environment where cell survival is low. To overcome such limitations, micro-sized injectable modularized units based on various biofabrication approaches as ideal delivery vehicles for cells and various growth factors have garnered compelling interest owing to their minimally-invasive nature, ease of packing cells, and improved cell retention efficacy. Several advancements have been made in fabricating various 3D biomimetic microscale carriers for cell delivery applications. In this review, we explicitly discuss the progress of the microscale cell carriers that potentially pushed the borders of TE, highlighting their design, ability to deliver cells and substantial tissue growth in situ and in vivo from different viewpoints of materials chemistry and biology. Finally, we summarize the perspectives highlighting current challenges and expanding opportunities of these innovative carriers.

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In vivo evaluation of an electrospun and 3D printed cellular delivery device for dermal wound healing

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 14 publications

References 33 publications

Manufacturing 3D Biomimetic Tissue: A Strategy Involving the Integration of Electrospun Nanofibers with a 3D‐Printed Framework for Enhanced Tissue Regeneration

Manufacturing 3D Biomimetic Tissue: A Strategy Involving the Integration of Electrospun Nanofibers with a 3D‐Printed Framework for Enhanced Tissue Regeneration

Combination of 3D Printing and Electrospinning Techniques for Biofabrication

Polymeric microcarriers for minimally-invasive cell delivery

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