In vivo Evaluation of Fibrous Collagen Dura Substitutes

Abstract

Dura substitutes are applied in duraplasty to repair lost or damaged dura. Collagen-based dura substitutes are mainstream products in both the US and Chinese markets. In this study, dura substitute devices with potential dura regeneration ability are evaluated. The dura substitutes are composed of fibrous type I collagen that were purified from bovine tendon. Physical and chemical characterization demonstrated that the tested dura substitute has desirable porous scaffolding structures and is composed of highly purified type I collagen. The collagen dura substitutes were further investigated in vivo with a rabbit model for 6 months to evaluate their safety and performance to repair and regenerate dura. No inflammation or infection was observed during the course of in vivo study. The integration of the collagen dura substitutes with surrounding tissue was normal as compared to native tissue. The macroscopic and microscopic histological assessments of the sampled animal tissue showed that the damaged dura were regenerated. The collagen dura substitutes were resorbed between 3 and 6 months along with newly regenerated dura. Both tissue adhesion and dura repair was the worst in blank control group as compared to those in the collagen dura substitutes. Taken together, regenerative collagen dura substitutes demonstrated with suitable physicochemical properties. The in vivo evaluation in a rabbit model further demonstrated the safety and performance of such substitutes for dura repair and regeneration.

Ex vivo confocal Raman microspectroscopy of porcinedura matersupported by optical clearing

“…[72] Moreover, glycerol interaction with collagen is important in the context of the suitability of the collagenous tissue as a dural graft in the repair of spinal dura mater defects. [73, 74]…”

“…[72] Moreover, glycerol interaction with collagen is important in the context of the suitability of the collagenous tissue as a dural graft in the repair of spinal dura mater defects. [73,74] The inherently weak Raman scattering efficiency and the high scattering of the turbid tissue limits CRM use to superficial layers. To overcome this lack and to extend Raman scattering probing to deeper tissues by using optical clearing method with the assistance of metallic nanoparticles can be further used.…”

Absorbable Artificial Dura Versus Nonabsorbable Artificial Dura in Decompressive Craniectomy for Severe Traumatic Brain Injury: A Retrospective Cohort Study in Two Centers

“…According to our clinical data, we consider that severe TBI patients are more likely to benefit from biological suturable nonabsorbable dura with good airtightness and elasticity for dura repair in DC. However, sutureless absorbable dura also has its advantages of small patches application in some locations difficult to reach, shorter surgery duration and better histomorphologic outcomes (45)(46)(47). A recent study reported a new absorbable dura made of Poly L Lactic Acid which shows good mechanical properties and represents better anti-adhesive property than NormalGEN in preclinical studies (48).…”

Electrospun Nanofibers for Dura Mater Regeneration: A Mini Review on Current Progress

“…It is made up of fibrous tissue, including collagenic and elastic fibers, and functions as a barrier to contain cerebrospinal fluid (CSF) within the cranial cavity. The dura mater can be divided into two layers [ 50 , 51 ]. The dense inner layer of the dura mater is the meningeal dura, which serves as a barrier by preventing the leakage of CSF and adhesion of brain tissue, guarding against superficial cerebral infection, and maintaining intracranial pressure.…”