In vivo evaluation of the toxic activity and genotoxicity of the Hymenaea courbaril L.’s resin in Drosophila melanogaster

Abstract

Due to the negative consequences carried by the usage of synthetic insecticides, a global interest into finding substitutes for these chemical compounds through natural products has arisen. When yielded to external attacks, plants generally produce metabolites to defend themselves. The physicochemical characteristics of this kind of compounds have allowed their usage as potential bioinsecticides. The Hymenaea courbaril L. (algarrobo) has proven to be a plant rich in metabolites with outstanding biological activity, in such a way that some of its extracts have been tested as insecticides. The goal of this study was to know the phytochemical composition of Hymenaea courbaril L.’s resin and perform evaluations in vivo of its toxic and genotoxic effects in the biological model Drosophila melanogaster . For this, two resin extracts were prepared and both a phytochemical analysis were carried out on them, having found in the ethanolic total extract the presence of terpenes, flavonoids and coumarins, while in the partial ethanolic extract only presence of terpenes and flavonoids was found. Drosophila larvae were submitted to different concentrations of the extracts and both the survival and the sexual ratio were evaluated, finding that larvae are more sensitive to the partial ethanolic extract. Subsequently, the induction of somatic mutation and mitotic recombination (SMART) was evaluated in the flies’ eyes. The most significant affectations at a genotoxic level were found when larvae were tested with the partial extract, indicating that possibly the coumarins absence makes this insect more susceptible to damages at a genetic material level.

Hymenaea courbaril resin-mediated gold nanoparticles as catalysts in organic dyes degradation and sensors in pharmaceutical pollutants

“…Traditionally this plant is commonly used to cure pain, inflammation, headache, colds, and bronchitis [ 28 ]. H. courbrail L. resin extracts have been reported in literature for the presence of good concentrations of flavonoids, terpenes and coumarins [ 29 ]. Taking the vast medicinal applications and lack of nanostructural study on H. courbrail L. resin into consideration, the current study was aimed at synthesizing HCRAuNPs via green nanotechnology utilizing aqueous extract.…”

Growth inhibitory, immunosuppressive, cytotoxic, and genotoxic effects of γ-terpinene on Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae)

“…For the management of insect pests, chemically synthesized pesticides are being extensively used 1 . Also, the widespread use of these pesticides has certain negative repercussions such as human health hazards, toxicity to ecologically beneficial organisms, water, soil, and air pollution, and development of resistance in pests 2 , 3 . Biopesticides, therefore, have grabbed the attention of scientific community owing to their low toxicity and biodegradability 4 with one such being the botanicals or plant-based pesticides.…”