In vivo Evaluation of the Repellency Effects of Nanoemulsion of Mentha piperita and Eucalyptus globulus Essential Oils against mosquitoes

Abstract

Introduction: The present study aimed to prepare Nanoemulsions of Mentha piperita and Eucalyptus globulus essential oils and comparison of the repellant activity of them with normal essential oils and DEET in the field conditions. Methods: To determine the protection and failure time of the essential oils and DEET in the field condition against natural population of night biting culicid mosquitoes, 4 human volunteers participated in night biting test. GC-MS was used to determine the essential oil components and the Dynamic Light Scattering device was used to measure droplet size and zeta potential. Results: The relative abundance of more common species captured in this study was 40.09% and 31.65% for Anopheles superpictus, and Culex pipiens, respectively. Based on the results, the protection time of nanoemulsions of M. piperita 50% against night biting mosquitoes was 4.96±0.21 h. Also, the protection for nanoemulsions essential oil 50% of E. globulus was 6.06±0.20 h. Comparison of the results showed that the protection time of nanoemulsions of M. piperita and E. globulus was significantly higher than of their normal essential oils (P˂0.01). Also, the protection time of DEET (as a gold standard) was significantly higher than of normal essential oil and nanoemulsions of M. piperita (P˂0.01), but there is no significant difference between DEET and nanoemulsions of E. globulus (P˃0.01). Conclusion: Due to the safety and biocompatibility of the nanoessential oils, and also relatively adequate and acceptable protection time, nanoemulsions of E. globulus and probably M. piperita can be considered as good repellents. It is recommended to do more research on these nanoemulsion repellents, as they may be good alternatives to DEET.

Field effectiveness of some essential oil emulsions against the whitefly Bemisia tabaci (GENN.) that infest potato plants in Egypt

“…This was consistent with the findings of (Heydari et al 2020;Barzegar et al 2018). However, the smallest size of the NE formulation of PNE obtained by (Shaker et al 2022) was about 66 nm, and the ENE obtained by (Mohammadi et al 2020) was about 103.9 nm, which is like the present research. Smaller particle size improves the NE penetration through the insect cuticle and plays a critical role in insecticidal activity.…”

Efficiency of essential oils emulsions against Whitefly Bemisia tabaci (GENN.) infesting potato plants under field conditions In Egypt

“…This was consistent with the ndings of (Heydari et al 2020; Barzegar et al 2018). However, the smallest size of the NE formulation of PNE obtained by (Shaker et al 2022) was about 66 nm, and ENE obtained by (Mohammadi et al 2020) was about 103.9 nm, which is like the present research. Smaller particle size improves the NE penetration through the insect cuticle and plays a critical role in insecticidal activity.…”

Essential Oil-Based Nano-Biopesticides: Formulation and Bioactivity against the Confused Flour Beetle Tribolium confusum

“…The repellent activity of EO-based nano-emulsions and gels has been largely investigated for insect vector management [48][49][50], though they may also be exploited for stored product pest control [51]. The area-preference trials highlighted good repellent activity toward T. confusum of the majority of the developed gels.…”