In vivo fate mapping of cryopreserved murine ovarian grafts

Chen, Chi Huang; Sb, Tan; Tzeng, Chii Ruey

doi:10.1186/s13048-014-0081-7

Cited by 7 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[32] Chen et al [33] recently reported that ovarian cryopreservation by slow freezing compromises ovarian reserve by cryoinjury and ischemia, evident at an early stage after transplantation. Oktem et al [25] found that vitrified human ovaries have fewer primordial follicles and produce less anti-Müllerian hormone than slow-frozen ovaries.…”

Section: Discussionmentioning

confidence: 99%

Comparison of vitrification and conventional slow freezing for cryopreservation of ovarian tissue with respect to the number of intact primordial follicles

Zhou

Zhang²,

Shi³

et al. 2016

Medicine

View full text Add to dashboard Cite

Background:Vitrification is the standard method for cryopreserving human oocytes and embryos, but its effects on ovarian tissue are uncertain. The purpose of this meta-analysis was to compare the proportion of intact primordial follicles in ovarian tissue cryopreserved with vitrification versus slow freezing.Methods:Medline, Cochrane, EMBASE, and Google Scholar databases were searched until November 11, 2014 using combinations of the search terms: ovarian tissue, cryopreservation, vitrification, follicle, follicles. Inclusion criteria were randomized controlled trails, two-arm prospective studies, and retrospective studies in which ovarian tissues were preserved by vitrification or conventional slow freezing. The primary outcome was the proportion of intact primordial follicles.Results:Six studies were included in the meta-analysis. The number of patients ranged from 3 to 20, and age ranged from 20 to 43 years. Total number of morphologically intact follicles ranged from 14 to 2058, among which 6 to 724 were primordial. The pooled odds ratio (OR) showed no significant difference in the proportion of intact primordial follicles after slow freezing or vitrification (OR = 1.228, 95% confidence interval [CI]: 0.769–1.961, P = 0.390). Sensitivity analysis using the leave-one-out approach indicated no considerable changes in the direction and magnitude of the pooled estimates when individual studies were excluded one at a time, indicating good reliability of the current analysis.Conclusions:Vitrification and slow freezing produce equivalent results with respect to intact primordial follicles for the cryopreservation of human ovarian tissue. However, the included studies varied in the cryopreservation protocols used.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of vitrification and conventional slow freezing for cryopreservation of ovarian tissue with respect to the number of intact primordial follicles

Zhou

Zhang²,

Shi³

et al. 2016

Medicine

View full text Add to dashboard Cite

show abstract

“…The donors were 3-week-old immature male FVB/N-Tg (PolII-luc) Ltc transgenic mice with an H-2 haplotype (H 2 q ). These mice were created by the transgenic service of Level Biotechnology (New Taipei City, Taiwan), and the generation process was described in our previous studies [11,13,14]. In brief, after pronuclear microinjection of the PolII-Luc transgene into the FVB/N embryos, they could encode a 712-bp mouse RNA polymerase II promoter (PolII) and a modi ed re y luciferase cDNA (Promega pGL-2).…”

Section: Micementioning

confidence: 99%

“…As described in our previous study, we used FVB/N-Tg (PolII-luc) Ltc transgenic mice and showed that BLI is a viable tool for assessing the e cacy of germ cell transplantation in vivo in a transgenic male mouse model [11]. Our team has used this technology to track the survival of mouse ovarian iso-and allografts [12], the effects of immunosuppressant treatment after allotransplantation of ovarian grafts [13], and the fate of cryopreserved murine ovarian grafts [14]. These previous studies mainly focused on ovarian grafts since the BLI technology is rarely used in experiments for fertility preservation of ITT.…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach of Tracing in Vivo Bioluminescence Imaging Expression of Vitrified Immature Testicular Tissue Grafts Until Adulthood: A Translational Transgenic Mouse Model

Liu

Lin

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background: The optimal method for cryopreserving immature testicular tissue (ITT) remains unknown and there is no standardized protocol. Controlled slow freezing remains the mainstream method of choice in human prepubertal male fertility preservation. Currently, the outcomes for ITT vitrification are conflicting, and most data are limited to in vitro animal studies.Methods: A total of 12 pairs of donor and recipient mice were included in our experiments. The donors were immature transgenic mice, and the recipients were wild-type male mice. In the vitrification group, ITT was vitrified and thawed before transplantation. In the control group, ITT was transplanted to the recipients immediately. After thawing, we measured the expression of apoptosis-related mRNA caspase-3. More importantly, we monitored to adulthood all the transplanted grafts in vivo using noninvasive bioluminescence imaging (BLI) technology. On day 31, we removed the grafts for evaluation via hematoxylin and eosin staining and immunohistochemistry (IHC).Results: We traced the survival of the grafts by in vivo BLI on days 1, 2, 5, 7, and 31 after transplantation. In both the vitrification and the control groups, bioluminescence decreased between days 2 and 5. Subsequently, the bioluminescence showed an upward trend until day 31. Compared with day 1, the bioluminescence was significantly stronger on day 31 after transplantation (P = 0.009). The differences between the two groups were constantly insignificant after analysis. These results indicate that both fresh and frozen–thawed testicular tissues can survive for at least 31 days after transplantation. Moreover, the vitrification group showed BLI signals comparable with those of fresh tissues. Compared with the control group, expression of the caspase-3 gene was significantly increased after vitrification (P = 0.04). Histology and IHC showed that both tissue structure and protein expression were intact in both groups.Conclusions: Transplanted vitrified ITT grafts could survive till adulthood with BLI intensity comparable to that of the fresh control. Intact cells and structures for spermatogenesis in vitrified ITT grafts were as well-preserved as those in the control group. This translational model of self-repairing vitrified ITT grafts in vivo, lends weight to the role of vitrification in prepubertal male fertility preservation.

show abstract

“…For example, embryo and egg freezing are not possible or are contraindicated in prepubertal females or those who cannot delay treatment for their primary condition (Jeruss and Woodruff 2009). For these patients, one experimental fertility option is ovarian tissue cryopreservation (OTC) (Backhus, et al 2007, Chen, et al 2014, De Vos, et al 2014, Dolmans, et al 2014, Ross, et al 2014, Stoop, et al 2014, Suzuki, et al 2015). OTC involves the removal of a whole ovary or cortical biopsies that are then cryopreserved by either slow freezing or vitrification (Rosendahl, et al 2011, Suzuki, et al 2015, Ting, et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…OTC involves the removal of a whole ovary or cortical biopsies that are then cryopreserved by either slow freezing or vitrification (Rosendahl, et al 2011, Suzuki, et al 2015, Ting, et al 2013). This tissue can be thawed at a later date and used to restore reproductive function (Backhus, et al 2007, Chen, et al 2014, De Vos, et al 2014, Dolmans, et al 2014, Ross, et al 2014, Stoop, et al 2014). OTC followed by orthotopic or heterotopic transplantation has resulted in restoration of endocrine function and fertility, with at least 60 live human births reported to date (Dittrich, et al 2015, Donnez and Dolmans 2015, Gamzatova, et al 2014, Smitz, et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Ovarian tissue transport to expand access to fertility preservation: from animals to clinical practice

et al. 2016

View full text Add to dashboard Cite

Primordial follicles dictate a female’s reproductive lifespan and therefore are central to fertility preservation for both endangered species as well as for individuals with fertility threatening conditions. Ovarian tissue containing primordial follicles can be cryopreserved and later thawed and transplanted back into individuals to restore both endocrine function and fertility. Importantly, increasing numbers of human live births have been reported following ovarian tissue cryopreservation and transplantation. A current limitation of this technology is patient access to sites that are approved or equipped to process and cryopreserve ovarian tissue – especially in larger countries or low resource settings. Here we review empirical evidence from both animal models and human studies that suggest that ovarian tissue can be transported at cold temperatures for several hours while still maintaining the integrity and reproductive potential of the primordial follicles within the tissue. In fact, several human live births have been reported in European countries using tissue that was transported at cold temperatures for up to 20 hours prior to cryopreservation and transplantation. Ovarian tissue transport, if implemented widely in clinical practice, could therefore expand both patient and provider access to emerging fertility preservation options.

show abstract

In vivo fate mapping of cryopreserved murine ovarian grafts

Cited by 7 publications

References 32 publications

Comparison of vitrification and conventional slow freezing for cryopreservation of ovarian tissue with respect to the number of intact primordial follicles

Comparison of vitrification and conventional slow freezing for cryopreservation of ovarian tissue with respect to the number of intact primordial follicles

A Novel Approach of Tracing in Vivo Bioluminescence Imaging Expression of Vitrified Immature Testicular Tissue Grafts Until Adulthood: A Translational Transgenic Mouse Model

Ovarian tissue transport to expand access to fertility preservation: from animals to clinical practice

Contact Info

Product

Resources

About