In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens

Carlson, Kristen; Chidley, Matthew D.; Sung, Kung-Bin; Descour, Michael R.; Gillenwater, Ann M.; Follen, Michele; Richards‐Kortum, Rebecca

doi:10.1364/ao.44.001792

Cited by 104 publications

(57 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Image-guiding bundles maintain the relative arrangements of the individual fibers throughout the length of the bundle, allowing transmission of an intensity image in pixilated form. Such bundles are commercially available and are commonly used for both epifluorescence 59 and scanning confocal or two-photon imaging 19,23,25,30,31,39,40,42,[60][61][62] . However, unlike in lenses or GRIN fibers, only intensity information is transmitted.…”

Section: Fiber Bundlesmentioning

confidence: 99%

Fiber-optic fluorescence imaging

et al. 2005

View full text Add to dashboard Cite

Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue. A challenge in the creation of such devices is the design and integration of miniaturized optical and mechanical components. Until recently, fiber-based fluorescence imaging was mainly limited to epifluorescence and scanning confocal modalities. Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging. An upcoming generation of fluorescence imaging devices will be based on microfabricated device components.Fiber-optic fluorescence imaging has become increasingly versatile over the last decade as fiber-based devices have declined in size but gained in functionality. Three classes of applications in live animal and human subjects provide the primary motivations for innovation in fiber-optic imaging. First, basic research on biological and disease processes would benefit enormously from instrumentation that permits cellular imaging under conditions in which conventional light microscopy cannot be used. Many cell types reside within hollow tissue tracts or deep within solid organs that are inaccessible to optical imaging without devices that can reach such locations in a minimally invasive manner. Flexible fiber-optic devices also allow handheld imaging and imaging in freely moving animals 1 . Second, fiber devices might be implanted within live subjects for long-term imaging studies. This capability will not only benefit research on the cellular effects of aging, development or experience, but also will lead to new in vivo assays for testing of drugs and therapeutics. By allowing examination of cells concurrently with observation of disease symptoms and animal behavior, fiber-optic imaging will permit studies correlating cellular properties and disease outcome in individual subjects over time and could reduce the numbers of animals needed. A third set of applications concerns development of minimally invasive clinical diagnostics and surgical procedures 2 . Although much work remains, fiber-optic instrumentation has already broadened the applicability of in vivo fluorescence imaging.The seeds for development of fiber optic in vivo imaging were planted by early uses of fiberoptics and fluorescence for chemical sensing and spectroscopy, including classic work on detection of intracellular redox states [3][4][5] . Use of fiber optics for remote sensing and spectroscopy remains strong today 6 , and has expanded to include bioluminescence detection 7 . The recent proliferation of fluorescent probes has widened the set of potential uses © 2005 Nature Publishing Group Correspondence should be addressed to M.J.S (mschnitz@stanford.edu). COMPETING INTERESTS STATEMENTThe authors declare that they have no co...

show abstract

Section: Fiber Bundlesmentioning

confidence: 99%

Fiber-optic fluorescence imaging

et al. 2005

View full text Add to dashboard Cite

show abstract

“…참고로 미국의 애리조나 대학의 광과학연구소에서 직경과 길이가 각각 7 mm와 20 mm인 전통적인 형식의 소형 대물 렌즈를 발표한 바 있지만 큰 관심을 끌지는 못했다. [9][10][11][12] 이는…”

Section: 서 론unclassified

Design of an Endoscopic Microscope Objective Composed of GRIN(Gradient-Index) Lens with Scanning Devices

Kim¹,

Rim²

2009

Korean Journal of Optics and Photonics

View full text Add to dashboard Cite

GRIN 렌즈로 구성된 주사방식의 내시현미경 대물렌즈의 설계김경정ㆍ임천석 Design of an EndoscopicWe present an attractive real time in-vivo endoscopic microscope with a resolution of submicron, in which two kinds of optical correcting plates are inserted to eliminate higher order spherical aberration and field curvature. And, since the conventional objective lens is replaced to GRIN lenses with diameter of 1 mm, the above endoscopic microscope can be effectively utilized to invade minimally for live animals. 는 미미한 실정이다. 독일의 막스플랑크 연구소 [2] , 미국의 벨 연구소 [3] , 미국의 코넬 [4] , 스텐퍼드 [5] , 하버드 대학 [6][7] , 영국의 옥스포드 대학

show abstract

“…The microscope has been developed with the aim to apply a typical solid state spectroscopic approach (by dielectric function classification) to the early detection of skin cancer, disease classification and similar applications. The LSCM is indeed considered to be useful in such respect, 18,19 also leading towards great very local and fast tools developed for this specific purpose. 20 We are in the middle of such an achievement, and we have yet to prove our approach useful and possible also for biologic tissues.…”

Section: Introductionmentioning

confidence: 99%

Supercontinuum ultra wide range confocal microscope for reflectance spectroscopy of living matter and material science surfaces

2011

View full text Add to dashboard Cite

We report the design and implementation of a new reflectance laser scanning confocal system with spectroscopy imaging capabilities. Confocal spectroscopy is achieved by using a very broad spectral range supercontinuum source capable of high precision reflectance data in the VIS-IR spectral range thanks to an almost achromatic optical layout. With this apparatus we collect each single scanning point as a whole spectrum in a continuous range, associated with the optical section imaging possibilities typical of a confocal set up. While such a microscope has been developed for bio medical analysis of human skin and other similar applications, first test results on solid samples produce spectroscopic results that, compared to analytical models based on the Abelés matrix transfer methods, show a very good agreement, opening new possibilities of a complete spectroscopic fingerprinting of samples with microscopic details. Copyright 2011 Author(s). This article is distributed under a Creative Commons Attribution 3.0 Unported License.

show abstract

In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens

Cited by 104 publications

References 18 publications

Fiber-optic fluorescence imaging

Fiber-optic fluorescence imaging

Design of an Endoscopic Microscope Objective Composed of GRIN(Gradient-Index) Lens with Scanning Devices

Supercontinuum ultra wide range confocal microscope for reflectance spectroscopy of living matter and material science surfaces

Contact Info

Product

Resources

About