In vivo gene delivery by electroporation

Prediction and optimization of gene transfection and drug delivery by electroporation

“…In contrast, clinical electrochemotherapy protocols have used multiple, short pulses at moderate field strengths. [6][7][8] This clinical protocol may be successful because the greater reliability of using protocols less sensitive to small variations in field strength outweighs the need to maximize uptake, since the drug used in these studies, bleomycin, is extremely potent at low intracellular concentrations. 8 …”

“…However, protocols are determined empirically and then collected in multivolume books of recipes that are often semi-anecdotal. [1][2][3][4][5] This situation, as well as recent and increasing application of electroporation to clinical chemotherapy and nonviral gene therapy, [6][7][8] suggest the need for methods to predict electroporation-enhanced molecular uptake, loss of cell viability and degree of transfection. To address this need, we (1) used a statistical approach to quantitatively predict electroporation-mediated gene transfection, molecular uptake and loss of cell viability; (2) validated it using a large independent data set from the literature; and (3) demonstrated how it can be used to rationally optimize electroporation protocols.…”

“…DNA transfection of cells in culture has been known for many years; more recent studies have also demonstrated electroporation-mediated drug delivery across skin, gene delivery in animals and targeted chemotherapy in human patients. [6][7][8][9] Correspondence: MR …”

In vivo electroporation-mediated transfer of interleukin-12 and interleukin-18 genes induces significant antitumor effects against melanoma in mice

“…Recently, some reports demonstrated that the electroporation is also useful for in vivo gene delivery. 3 Marker genes were successfully transferred into normal tissues of animals including skeletal muscle, 4,5 liver, 6 skin 7 and cornea. 8 Tumors implanted in animals were also revealed to be good targets for in vivo electroporation; melanoma, 9 hepatocellular carcinoma, 10 and glioma 11 resulting in remarkable therapeutic outcome.…”

Electroporation-mediated and EBV LMP1-regulated gene therapy in a syngenic mouse tumor model

“…[30][31][32][33] Furthermore, in vivo electroporation is a safe and nontoxic delivery system. 34 This has been used for the efficient delivery of plasmid DNA encoding chemotherapeutic agents, such as cytokine IL12, resulting in tumor regression, long-term animal survival, and resistance to challenge. 35 In this study, delivery of the therapeutic cassette by in vivo electroporation into LMP1-expressing tumors also showed a therapeutic effect.…”