In vivo grafting of large engineered heart tissue patches for cardiac repair

Jabbour, Richard J.; Owen, Thomas J.; Pandey, Pragati; Reinsch, Marina; Wang, Brian; King, Oisín; Couch, Liam S.; Pantou, Dafni; Pitcher, David S.; Chowdhury, Rasheda A.; Pitoulis, Fotios G.; Handa, Balvinder S.; Kit‐Anan, Worrapong; Perbellini, Filippo; Myles, Rachel C.; Stuckey, Daniel J; Dunne, Michael; Shanmuganathan, Mayooran; Peters, Nicholas S.; Ng, Fu Siong; Weinberger, Florian; Terracciano, Cesare M.; Smith, Godfrey L.; Eschenhagen, Thomas; Harding, Siân E.

doi:10.1172/jci.insight.144068

Cited by 38 publications

(26 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even though no arrhythmic effects were observed, a sufficient electrical coupling was not obtained in these early approaches. 91 In a proof-of-concept study, engineered cardiac microtissue was utilized for transmural heart wall replacement. Transmural defects were generated using an aortic punch that resulted in a 6 mm diameter injury.…”

Section: Engineered Cardiac Microtissuementioning

confidence: 99%

“…Here, the cardiac patch engrafted into the host myocardium and led to improved left ventricular function. Even though no arrhythmic effects were observed, a sufficient electrical coupling was not obtained in these early approaches 91 . In a proof‐of‐concept study, engineered cardiac microtissue was utilized for transmural heart wall replacement.…”

Section: Engineered Cardiac Microtissuementioning

confidence: 99%

See 1 more Smart Citation

Accelerating cardiovascular research: recent advances in translational 2D and 3D heart models

Mohr

Thum

Bär

2022

European J of Heart Fail

View full text Add to dashboard Cite

In vitro modelling the complex (patho‐) physiological conditions of the heart is a major challenge in cardiovascular research. In recent years, methods based on three‐dimensional (3D) cultivation approaches have steadily evolved to overcome the major limitations of conventional adherent two‐dimensional (2D) monolayer cultivation. These 3D approaches aim to study, reproduce or modify fundamental native features of the heart such as tissue organization and cardiovascular microenvironment. Therefore, these systems have great potential for (patient‐specific) disease research, for the development of new drug screening platforms, and for the use in regenerative and replacement therapy applications. Consequently, continuous improvement and adaptation is required with respect to fundamental limitations such as cardiomyocyte maturation, scalability, heterogeneity, vascularization, and reproduction of native properties. In this review, 2D monolayer culturing and the 3D in vitro systems of cardiac spheroids, organoids, engineered cardiac microtissue and bioprinting as well as the ex vivo technique of myocardial slicing are introduced with their basic concepts, advantages, and limitations. Furthermore, recent advances of various new approaches aiming to extend as well as to optimize these in vitro and ex vivo systems are presented.

show abstract

Section: Engineered Cardiac Microtissuementioning

confidence: 99%

Section: Engineered Cardiac Microtissuementioning

confidence: 99%

Accelerating cardiovascular research: recent advances in translational 2D and 3D heart models

Mohr

Thum

Bär

2022

European J of Heart Fail

View full text Add to dashboard Cite

show abstract

“…The safety concern of cardiac patch therapy was limited to arrhythmias, which were generally transient and non-fatal [129][130][131]. It is noteworthy that a recently published study has revealed the efficacy of upscaled engineered heart tissue to improve left ventricular function and reduce the infarct size in the context of ischemic myocardial disease without documenting a significant difference in arrhythmogenicity, compared to a cell-free patch group in a rabbit model [132].…”

Section: Conflicts Of Interestmentioning

confidence: 99%

Enhancing Mesenchymal Stem Cells (MSCs) for Therapeutic Purposes

2022

View full text Add to dashboard Cite

show abstract

“…With the vasculature or chambers inside, natural cardiac tissue is a hierarchically aligned structure with conductivity and strength, which is responsible to propagate and respond to electrical impulses by synchronized contractions that produce forces to pump blood. It challenges cardiac tissue engineering to construct engineered scaffolds with appropriate conductivity and stiffness to maintain physiological and contractile loads of the heart …”

Section: Introductionmentioning

confidence: 99%

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

et al. 2022

View full text Add to dashboard Cite

With permanent heart muscle injury or death, myocardial infarction (MI) is complicated by inflammatory, proliferation and remodeling phases from both the early ischemic period and subsequent infarct expansion. Though in situ re-establishment of blood flow to the infarct zone and delays of the ventricular remodeling process are current treatment options of MI, they fail to address massive loss of viable cardiomyocytes while transplanting stem cells to regenerate heart is hindered by their poor retention in the infarct bed. Equipped with heart-specific mimicry and extracellular matrix (ECM)-like functionality on the network structure, hydrogels leveraging tissue-matching biomechanics and biocompatibility can mechanically constrain the infarct and act as localized transport of bioactive ingredients to refresh the dysfunctional heart under the constant cyclic stress. Given diverse characteristics of hydrogel including conductivity, anisotropy, adhesiveness, biodegradability, self-healing and mechanical properties driving local cardiac repair, we aim to investigate and conclude the dynamic balance between ordered architectures of hydrogels and the post-MI pathological milieu. Additionally, our review summarizes advantages of heart-tailored architectures of hydrogels in cardiac repair following MI. Finally, we propose challenges and prospects in clinical translation of hydrogels to draw theoretical guidance on cardiac repair and regeneration after MI.

show abstract

In vivo grafting of large engineered heart tissue patches for cardiac repair

Abstract: Calcium transients. GCaMP6f EHTs were analyzed using standard methods (see Supplemental Methods), and parameters were calculated in pClamp. GCaMP6f cells were obtained courtesy of the Conklin laboratory (Gladstone Institutes,

Cited by 38 publications

References 39 publications

Accelerating cardiovascular research: recent advances in translational 2D and 3D heart models

Accelerating cardiovascular research: recent advances in translational 2D and 3D heart models

Enhancing Mesenchymal Stem Cells (MSCs) for Therapeutic Purposes

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

Contact Info

Product

Resources

About