In vivo imaging

Laskowski, Claudia J.; Bradke, Frank

doi:10.1016/j.expneurol.2012.07.007

Cited by 32 publications

(9 citation statements)

References 33 publications

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“…In vivo optical imaging in the spinal cord represents a radically different approach to study axonal responses to injury as it allows for the examination of the same axons in living animals over time (Laskowski and Bradke, 2013). The first of such a study, using wide-field fluorescence microscopy in conjunction with a pinprick lesion, led to the discovery of acute axon degeneration and provided the first time-lapse recordings of axon regeneration in the injured mammalian CNS (Kerschensteiner et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

A Surviving Intact Branch Stabilizes Remaining Axon Architecture after Injury as Revealed by In Vivo Imaging in the Mouse Spinal Cord

Lorenzana

Lee

Mui

et al. 2015

Neuron

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SUMMARY The complex morphology of axons presents a challenge in understanding axonal responses to injury and disease. By in vivo 2-photon imaging of spinal dorsal column sensory axons, we systematically examined the effect of injury location relative to the main bifurcation point on axon degeneration and regeneration following highly localized laser injuries. Retrograde but not anterograde degeneration was strongly blocked at the bifurcation point at both the acute and subacute phases. Eliminating either the ascending or descending branch led to a poor regenerative response while eliminating both led to a strong regenerative response. Thus, a surviving intact branch suppresses both retrograde degeneration and regeneration of the injured branch, thereby stabilizing the remaining axon architecture. Regenerating axons exhibited a dynamic pattern with alternating phases of regeneration and pruning over a chronic period. In vivo imaging continues to reveal new insights on axonal responses to injury in the mammalian spinal cord.

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Section: Introductionmentioning

confidence: 99%

A Surviving Intact Branch Stabilizes Remaining Axon Architecture after Injury as Revealed by In Vivo Imaging in the Mouse Spinal Cord

Lorenzana

Lee

Mui

et al. 2015

Neuron

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“…As epothilone B induced microtubule polymerization and axon growth in cultured neurons, we assessed its ability to promote axon regeneration after rodent SCI. In vivo imaging of adult transgenic mice, expressing GFP in spinal cord dorsal column axons (26, 27), revealed that transected axons of epothilone B injected animals exhibited significantly fewer retraction bulbs (Fig. 3, C and D), reduced axonal dieback and increased regenerative growth (Fig.…”

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confidence: 99%

Systemic administration of epothilone B promotes axon regeneration after spinal cord injury

Ruschel

Hellal

Flynn

et al. 2015

Science

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394

356

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After central nervous system (CNS) injury, inhibitory factors in the lesion scar and a poor axon growth potential prevent axon regeneration. Microtubule stabilization reduces scarring and promotes axon growth. However, the cellular mechanisms of this dual effect remain unclear. Here, delayed systemic administration of a blood-brain barrier permeable microtubule stabilizing drug, epothilone B, decreased scarring after rodent spinal cord injury (SCI) by abrogating polarization and directed migration of scar-forming fibroblasts. Conversely, epothilone B reactivated neuronal polarization by inducing concerted microtubule polymerization into the axon tip, which propelled axon growth through an inhibitory environment. Together, these drug elicited effects promoted axon regeneration and improved motor function after SCI. With recent clinical approval, epothilones hold promise for clinical use after CNS injury.

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“…The multimodality imaging technique allows for studying acute pathological events following a spinal cord lesion, and the development of implanted spinal chamber enables long-term imaging for chronic spinal cord preparations. Therefore, in vivo imaging allows the direct observation of dynamic regenerative events of individual stem cells after traumatic injury in the living subject [107]. We predict that future improvement in molecular imaging will make important contributions to our understanding of stem cells' transplantation and allow us to assess the therapeutic effect on a molecular scale.…”

Section: Perspective and Summarymentioning

confidence: 99%

Molecular Imaging in Stem Cell Therapy for Spinal Cord Injury

Song

Tian

Zhang

2014

BioMed Research International

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Spinal cord injury (SCI) is a serious disease of the center nervous system (CNS). It is a devastating injury with sudden loss of motor, sensory, and autonomic function distal to the level of trauma and produces great personal and societal costs. Currently, there are no remarkable effective therapies for the treatment of SCI. Compared to traditional treatment methods, stem cell transplantation therapy holds potential for repair and functional plasticity after SCI. However, the mechanism of stem cell therapy for SCI remains largely unknown and obscure partly due to the lack of efficient stem cell trafficking methods. Molecular imaging technology including positron emission tomography (PET), magnetic resonance imaging (MRI), optical imaging (i.e., bioluminescence imaging (BLI)) gives the hope to complete the knowledge concerning basic stem cell biology survival, migration, differentiation, and integration in real time when transplanted into damaged spinal cord. In this paper, we mainly review the molecular imaging technology in stem cell therapy for SCI.

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In vivo imaging

Cited by 32 publications

References 33 publications

A Surviving Intact Branch Stabilizes Remaining Axon Architecture after Injury as Revealed by In Vivo Imaging in the Mouse Spinal Cord

A Surviving Intact Branch Stabilizes Remaining Axon Architecture after Injury as Revealed by In Vivo Imaging in the Mouse Spinal Cord

Systemic administration of epothilone B promotes axon regeneration after spinal cord injury

Molecular Imaging in Stem Cell Therapy for Spinal Cord Injury

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