In Vivo Imaging of Embryonic Development in the Mouse Eye by Ultrasound Biomicroscopy

Foster, F. Stuart; Zhang, Mingyu; Duckett, Allison S; Cucevic, Viviene; Pavlin, Charles J.

doi:10.1167/iovs.02-0911

Cited by 72 publications

(49 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies have already indicated the possibility of obtaining real-time in vivo skeletal muscle images of rats using high-resolution ultrasound. For instance, UBM has been used to study embryonic development through in utero images of small animals (Foster, 2003;Foster et al, 2002) and Witte et al (Witte et al, 2004) used UBM to describe the coordination of individual fibers of a rat and a mouse through ex vivo tests.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound biomicroscopy for biomechanical characterization of healthy and injured triceps surae of rats

Peixinho

Ribeiro

Resende

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

Recently, Eng et al. (Eng et al., 2008) presented a study that examined muscle architecture and fiber type in the rat hindlimb to define the functional specialization of each muscle. Nevertheless, a literature search for studies quantifying skeletal muscle architectural parameters of rats in vivo was unsuccessful. Studies with animals related to the degeneration-regeneration process subsequent to muscle injury are restricted to in vitro analysis and do not permit longitudinal follow-up. SUMMARY This work describes the use of ultrasound biomicroscopy (UBM) to follow up the degeneration-regeneration process after a laceration injury induced in the lateral gastrocnemius (LG) and soleus (SOL) muscles of rats. UBM (40MHz) images were acquiredand used for biomechanical characterization of muscular tissue, specifically using pennation angle (PA) and muscle thickness (MT). The animals were distributed in three groups: the variability group (VG; N5), the gastrocnemius injured group (GG; N6) and the soleus injured group (SG; N5). VG rats were used to assess data variability and reliability (coefficients of variation of 9.37 and 3.97% for PA and MT, respectively). GG and SG rats were submitted to the injury protocol in the LG and SOL muscles of the right legs, respectively. UBM images of muscles of both legs were acquired at the following time points: before and after injury (immediately, 7, 14, 21 and 28days). We observed an increase in PA for the non-injured leg 28days after injury for both GG and SG rats (GG10.68 to 16.53deg and SG9.65 to 14.06deg; P<0.05). Additionally, MT presented a tendency to increase (GG2.92 to 3.13mm and SG2.12 to 2.35mm). Injured legs maintained pre-injury PA and MT values. It is suggested that a compensatory hypertrophic response due to the overload condition imposed to healthy leg. The results indicate that UBM allows qualitative and quantitative muscle differentiation among healthy and injured muscle at different stages after lesion.

show abstract

Section: Introductionmentioning

confidence: 99%

Ultrasound biomicroscopy for biomechanical characterization of healthy and injured triceps surae of rats

Peixinho

Ribeiro

Resende

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Unauthorized reproduction of this article is prohibited. [12], cow [29], and human [27] data. The gray lines fit the periods of exponential growth in each species.…”

mentioning

confidence: 99%

Active Self-Assembly of Simple Units Using an Insertion Primitive

Dabby¹,

Chen²

2013

Proceedings of the Twenty-Fourth Annual ACM-SIAM Symposium on Discrete Algorithms

View full text Add to dashboard Cite

While computer science has given us a framework for determining the complexity and difficulty of solving computational problems, we do not yet have a theoretical framework for knowing what actions, behaviors, and life-like qualities can emerge from a given set of simple modular units. There has been much interest in developing models for programming active self-assembly processes in both the reconfigurable robotics community and the nanotechnology community. With respect to materials science and nanotechnology, the models proposed to date are either not yet implementable with our current understanding of synthetic chemistry or those that are implementable are limited to a set of features that do not capture the power of active components. Prior implementable models of molecular assembly only considered the passive behaviors of attaching and detaching from a complex.Inspired by the algorithmic tile assembly model [Winfree, 1996] and the graph grammar assembly model [Klavins et al., 2004], we describe a formal model for studying the complexity of self-assembled structures with active molecular components. In particular, we add an insertion primitive and we show a direct mapping of our model to a molecular implementation using DNA. We show that the expressive power of this language is stronger than regular languages, but at most as strong as context free grammars. Here, we explore the trade-off between the complexity of the system (in terms of the number of unit types), and the behavior of the system and speed of its assembly. We find that we can grow a line of any given length n in expected time O(log 3 n) using O(log 2 n) monomers. If we grow a line with k insertion rules, either the expected final length is infinite or the expected length at time t is at most (2t + 2) k 2 , which is polynomial in t.

show abstract

“…To quantify this effect, the eye volumes of the embryos were quantified with our previously published technique [54]. Briefly, the eye was modeled as an oblate spheroid [55], and the minor (along the optical axis) and major (orthogonal to the optical axis) axes lengths were obtained from the scaled OCT and OPT images. The volume was calculated by: …”

Section: Quantification Of Eye Volumesmentioning

confidence: 99%

Applicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography

Singh¹,

Raghunathan²,

Piazza³

et al. 2016

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:We present an analysis of imaging murine embryos at various embryonic developmental stages (embryonic day 9.5, 11.5, and 13.5) by optical coherence tomography (OCT) and optical projection tomography (OPT). We demonstrate that while OCT was capable of rapid highresolution live 3D imaging, its limited penetration depth prevented visualization of deeper structures, particularly in later stage embryos. In contrast, OPT was able to image the whole embryos, but could not be used in vivo because the embryos must be fixed and cleared. Moreover, the fixation process significantly altered the embryo morphology, which was quantified by the volume of the eye-globes before and after fixation. All of these factors should be weighed when determining which imaging modality one should use to achieve particular goals of a study. References and links1. J. Beckers, W. Wurst, and M. H. de Angelis, "Towards better mouse models: enhanced genotypes, systemic phenotyping and envirotype modelling," Nat. Rev. Genet. 10(6), 371-380 (2009 440-443 (2000). 4. K. Paigen and J. T. Eppig, "A mouse phenome project," Mamm. Genome 11(9), 715-717 (2000). Proc. Natl. Acad. Sci. U.S.A. 91(9), 3530-3533 (1994). 9. M. Dhenain, S. W. Ruffins, and R. E. Jacobs, "Three-dimensional digital mouse atlas using high-resolution MRI," Dev. Biol. 232(2), 458-470 (2001 95-105 (1999). 33. U. Morgner, W. Drexler, F. X. Kärtner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, "Spectroscopic optical coherence tomography," Opt. Lett. 25(2), 111-113 (2000). 34. S. Rugonyi, C. Shaut, A. Liu, K. Thornburg, and R. K. Wang, "Changes in wall motion and blood flow in the outflow tract of chick embryonic hearts observed with optical coherence tomography after outflow tract banding and vitelline-vein ligation," Phys.

show abstract

In Vivo Imaging of Embryonic Development in the Mouse Eye by Ultrasound Biomicroscopy

Abstract: UBM allows noninvasive assessment of ocular morphogenesis in vivo and can be used to calculate relative growth rates of ocular structures.

Cited by 72 publications

References 16 publications

Ultrasound biomicroscopy for biomechanical characterization of healthy and injured triceps surae of rats

Ultrasound biomicroscopy for biomechanical characterization of healthy and injured triceps surae of rats

Active Self-Assembly of Simple Units Using an Insertion Primitive

Applicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography

Contact Info

Product

Resources

About