In vivo/in vitro labeling of red blood cells with 99mTc

Abstract

Red blood cells (RBC) were labeled with 99mTc in vitro after in vivo pretreatment with a stannous solution. The whole in vivo/in vitro procedure took 30-40 min. Pretinning was carried out by injection of either 6 mg DTPA and 0.6 mg Sn++ (Sn-DTPA) or 5 mg pyrophosphate and 0.7 mg Sn++ (Sn-PYP). The results of 1,356 patients were evaluated. The labeling yield was 89.7% (mean) after pretinning with Sn-DTPA and 88.2% following Sn-PYP pretreatment, the median values being 94% and 92%, respectively. The new method was successfully used in over 2,000 patients who had a radionuclide-ventriculography and in 38 patients studied for localization of occult gastrointestinal bleeding. Adverse side effects have never been observed.

Factorial phase analysis of ventricular contraction using equilibrium radionuclide angiography images

“…Erythrocytes were labeled using a modified semi in vivo/in vitro technique with 740-925 MBq of Tc-99m, applied with UltraTag 1 (Mallinckrodt Inc., St. Louis, MO) [32,33]. The EKG was continuously monitored to ensure the precise R-wave detection during image acquisition.…”

Normality Index of Ventricular Contraction Based on a Statistical Model from FADS

“…Erythrocytes were marked applying an in vivo/in vitro modified technique with 740 to 925 MBq of Tc-99m, using an UltraTag Kit [46, 47]. EKG was continuously monitored to synchronize images acquisition with the R wave.…”

Bone scan and red blood cell scan in a patient with epidermal naevus syndrome

“…Biopsy of the left tibia demonstrated bone haemangioma. For in vivo/in vitro labelling of red blood cells we injected 6 mg diethylene triamine pentaacetic acid (DTPA) with 0.6 mg Sn 2 + (Bauer et al 1983) and sampled at 30 rain later 5 ml heparinized blood (40-60 IU/ml). Together with 5 ml isotonic NaC1 solution and 900 MBq 99mTc the red cell solution was incubated at 320-35 ° C for 15 min.…”