In Vivo Induction of Primary DNA Lesions upon Subchronic Oral Exposure to Multi-walled Carbon Nanotubes

Gerencsér, Gellért; Varjas, Tímea; Szendi, Katalin; Varga, Csaba

doi:10.21873/invivo.11006

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…The route of exposure is a key factor of the genotoxicity of a chemical due to its mode of action and selecting the appropriate administration route may be important when assaying multiple organs [363,364]. In rodents, different routes of exposure have been used to study the genotoxicity of different chemicals or nanomaterials, e.g., intravenous/systemic [365][366][367], intraperitoneal [368][369][370][371], oral [372][373][374][375] as well as inhalation [376][377][378]. [379], which is in accordance with EU Directive 2010/63/EU [96].…”

Section: Laboratory Mammalsmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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