In Vivo Inhibition of <i>Porphyromonas gingivalis</i> Growth and Prevention of Periodontitis With Quorum‐Sensing Inhibitors

Cho, Young-Jae; Song, He Sun; Amara, Heithem Ben; Choi, Bong‐Kyu; E, Ryu; Cho, Young-Ah; Seol, Yang‐Jo; Lee, Yong‐Moo; Ku, Young; Rhyu, In‐Chul; Koo, Ki‐Tae

doi:10.1902/jop.2016.160070

Cited by 26 publications

(29 citation statements)

References 48 publications

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“…Here, BMK‐Q101, a newly synthesized furanone, was chosen because of its improved properties to prevent biofilm growth of oral bacteria in comparison with a reference furanone compound previously investigated . This assumption was validated through in vitro experiments demonstrating that BMK‐Q101 not only significantly inhibits the AI‐2 activity of F. nucleatum ATCC 25586 – a major player of biofilm maturation – but also impairs biofilm growth of P. gingivalis ATCC 33227 and F. nucleatum ATCC 25586 .…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, D‐ribose was combined with BMK‐Q101 to potentialize AI‐2 inhibition in the animal model used in this experiment. As furanone is soluble in alcohol but not in water, BMK‐Q101 was not mixed with the drinking water; rather, it was orally administered, with CMC as vehicle, to avoid any side effects of alcohol abuse in the mice .…”

Section: Discussionmentioning

confidence: 99%

“…Protocols including diet, ligature, and oral infection by oral gavage have been proposed in order to experimentally inoculate periodontal pathogens in the oral cavity of animals . Infection of the oral cavity by topical administration of P. gingivalis is a well‐established model and has been previously used to explore the beneficial modulation of periodontitis by furanone and D‐ribose . Recognizing the polybacterial nature of periodontal disease and that AI‐2 is, in essence, exchanged between various species in the biofilm , a mouse model in which periodontitis is induced by mixed infection was created to study if BMK‐Q101 and D‐ribose could modulate disease manifestations.…”

Section: Discussionmentioning

confidence: 99%

“…CTAnalyzer software (Version 1.15.4.0+; Skyscan) was then used to open the images. Measurements were performed twice at 3‐wk intervals by a masked trained examiner for linear and volumetric evaluation of alveolar bone loss in right and left interproximal areas between maxillary first and second molars (M1–M2) and between maxillary second and third molars (M2–M3) .…”

Section: Methodsmentioning

confidence: 99%

“…Because of the high toxicity of the natural compounds, efforts have been directed toward synthesizing brominated furanone derivates with reduced toxicity and an improved capacity to limit bacterial biofilm growth . Recently, it was demonstrated that synthesized brominated furanones and D‐ribose not only reduce the biomass and depth of periodontopathogens biofilm in vitro , but also attenuate alveolar bone loss in vivo . Although molecules that inhibit AI‐2 signaling between P. gingivalis and F. nucleatum have been demonstrated to prevent the growth of P. gingivalis / F. nucleatum biofilm , these inhibitors have not yet been investigated in in vivo settings where periodontitis is induced by P. gingivalis / F. nucleatum co‐infection.…”

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confidence: 99%

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Effects of quorum‐sensing inhibition on experimental periodontitis induced by mixed infection in mice

Amara

Song

et al. 2018

European J Oral Sciences

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This study aimed to verify, in in vivo settings, whether quorum-sensing inhibition molecules could attenuate alveolar bone loss induced by Porphyromonas gingivalis/Fusobacterium nucleatum co-infection and reduce the bacterial colonization of periodontal tissues. In BALB/c mice, periodontitis was induced through oral inoculation with P. gingivalis and F. nucleatum six times during a 42-d period. Quorum sensing inhibitors (a furanone compound and D-ribose) were administered simultaneously with bacterial infection. Linear and volumetric modifications of interproximal alveolar bone levels were compared between groups using micro-computed tomography. Total bacteria, and P. gingivalis and F. nucleatum DNA in periodontal tissues, were quantified using real-time PCR. Radiographic linear measurements demonstrated a significant reduction of alveolar bone loss, of approximately 40%, in mice treated with quorum sensing inhibitors when compared with the co-infection group. This was confirmed by a significant increase of residual bone volume in the test group. While total bacterial genes in the treatment group significantly decreased by 93% in periodontal tissue samples when quorum sensing inhibitors were administered, no significant differences of P. gingivalis DNA were found. Quorum sensing inhibitors reduced periodontal breakdown and bacterial infection in periodontal tissues after co-infection with P. gingivalis and F. nucleatum.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of quorum‐sensing inhibition on experimental periodontitis induced by mixed infection in mice

Amara

Song

et al. 2018

European J Oral Sciences

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MyD88 exacerbates inflammation‐induced bone loss by modulating dynamic equilibrium between Th17/Treg cells and subgingival microbiota dysbiosis

Hsiao,

Huang,

Huang

et al. 2024

Journal of Periodontology

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BackgroundThis study aimed to investigate the contribution of myeloid differentiation primary‐response gene 88 (MyD88) on the differentiation of T helper type 17 (Th17) and regulatory T (Treg) cells and the emerging subgingival microbiota dysbiosis in Porphyromonas gingivalis‐induced experimental periodontitis.MethodsAlveolar bone loss, infiltrated inflammatory cells, immunostained cells for tartrate‐resistant acid phosphatase (TRAP), the receptor activator of nuclear factor‐kB ligand (RANKL), and osteoprotegerin (OPG) were quantified by microcomputerized tomography and histological staining between age‐ and sex‐matched homozygous littermates (wild‐type [WT, Myd88+/+] and Myd88−/− on C57BL/6 background). The frequencies of Th17 and Treg cells in cervical lymph nodes (CLNs) and spleen were determined by flow cytometry. Cytokine expression in gingival tissues, CLNs, and spleens were studied by quantitative polymerase chain reaction (qPCR). Analysis of the composition of the subgingival microbiome and functional annotation of prokaryotic taxa (FAPROTAX) analysis were performed.ResultsP. gingivalis‐infected Myd88−/− mice showed alleviated bone loss, TRAP+ osteoclasts, and RANKL/OPG ratio compared to WT mice. A significantly higher percentage of Foxp3+CD4+ T cells in infected Myd88−/− CLNs and a higher frequency of RORγt+CD4+ T cells in infected WT mice was noted. Increased IL‐10 and IL‐17a expressions in gingival tissue at D14–D28 then declined in WT mice, whereas an opposite pattern was observed in Myd88−/− mice. The Myd88−/− mice exhibited characteristic increases in gram‐positive species and species having probiotic properties, while gram‐negative, anaerobic species were noted in WT mice. FAPROTAX analysis revealed increased aerobic chemoheterotrophy in Myd88−/− mice, whereas anaerobic chemoheterotrophy was noted in WT mice after P. gingivalis infection.ConclusionsMyD88 plays an important role in inflammation‐induced bone loss by modulating the dynamic equilibrium between Th17/Treg cells and dysbiosis in P. gingivalis‐induced experimental periodontitis.

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Targeting Biofilms in Orthopedic Infection

Goswami

Parvızı

2019

Targeting Biofilms in Translational Research, Device Development, and Industrial Sectors

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In Vivo Inhibition of Porphyromonas gingivalis Growth and Prevention of Periodontitis With Quorum‐Sensing Inhibitors

Abstract: Use of QSIs in the mice infection model showed a reduction of bone breakdown and a decrease in the number of bacteria in vivo, suggesting that QSIs can be a new approach to prevention and treatment of periodontitis.

Cited by 26 publications

References 48 publications

Effects of quorum‐sensing inhibition on experimental periodontitis induced by mixed infection in mice

Effects of quorum‐sensing inhibition on experimental periodontitis induced by mixed infection in mice

MyD88 exacerbates inflammation‐induced bone loss by modulating dynamic equilibrium between Th17/Treg cells and subgingival microbiota dysbiosis

Targeting Biofilms in Orthopedic Infection

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