In Vivo Kinetics of Simultaneously Injected 111In- and 51Cr-Labelled Human Platelets: On the Significance of the Platelet Isolation Yield from Blood Prior to Labelling

Schmidt, Kjeld; Rasmussen, J. W.; Rasmussen, Anders Mølgaard; Arendrup, Henrik; Lorentzen, Martin

doi:10.1007/978-94-009-6027-5_13

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…An alternative explanation could be poor labelling of senescent platelets or heavy labelling (or a surplus) of young (or large) platelets, the effect of which, however, might be counteracted by preferential splenic sequestration of young or large platelets. We have previously shown that large platelets, upon injection, seem to leave the circulation more readily than small platelets (17). However, that the platelet isolation yield prior to labelling might have influenced the initial course of activity was not supported by the present study.…”

Section: Discussioncontrasting

confidence: 90%

Kinetics of ¹¹¹In‐labelled platelets in healthy subjects

Schmidt

Rasmussen

1985

Scandinavian Journal of Haematology

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25 healthy subjects underwent platelet kinetic and scintigraphic studies following injection of 111In‐labelled platelets. Using the multiple hit model, platelet mean lifetime averaged 185.3 hours (range 141.2–225.6), and platelet in vivo recovery averaged 56 % (range 42–89). The platelet disappearance pattern was almost linear. However, in comparison with simultaneously injected 51Cr‐labelled platelets (7 subjects studied), the 111In‐platelet survival curves were slightly more curved. Our results indicate that this may be ascribed to a slight degree of elution of 111In from the circulating platelets. The plateau or even slight increase in circulating activity following the initial steep decline in activity appears mainly to reflect reversible sequestration of platelets in the spleen and maybe in the liver. Using a semiquantitative scintigraphic approach, we estimated 45 %, 25 % and 30 % platelet destruction to occur in the spleen, liver and bone marrow, respectively. Our results indicate that the discrepancy between the course of circulating platelet‐bound activity and of chest wall activity reflects platelet destruction in the bone marrow.

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Section: Discussioncontrasting

confidence: 90%

Kinetics of ¹¹¹In‐labelled platelets in healthy subjects

Schmidt

Rasmussen

1985

Scandinavian Journal of Haematology

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“…Other investigators, however, do not support this concept (13,14). There is a certain degree of correlation between platelet volume and platelet density (6,11,15,16). Some studies support the concept that large platelets are younger than small ones (16,17), while others do not (18,19).…”

Section: Discussionmentioning

confidence: 98%

“…Platelet isolation from blood and labelling with oxine and 51Cr-sodium chromate in a buffer medium was performed as previously described (5). The platelet isolation procedure is based on stepwise centrifugation yielding fractions of platelets of increasing size (5, 6). In group D, the small platelets were labelled with 51Cr and the large ones with lllIn, as previously described (6); in the other groups the labelled platelets represented the total platelet population of the blood.…”

Section: Isolation and Labelling Of Plateletsmentioning

confidence: 99%

“…The platelet isolation procedure is based on stepwise centrifugation yielding fractions of platelets of increasing size (5, 6). In group D, the small platelets were labelled with 51Cr and the large ones with lllIn, as previously described (6); in the other groups the labelled platelets represented the total platelet population of the blood. The methods for calculation of platelet isolation yield and of the fractions of platelet and erythrocyte-bound activities in the injected platelet suspensions have been described elsewhere (5).…”

Section: Isolation and Labelling Of Plateletsmentioning

confidence: 99%

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Are Young Platelets Released in Excess from the Spleen in Response to Short‐Term Physical Exercise?

Schmidt

Rasmussen

1984

Scandinavian Journal of Haematology

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With a view to throwing some light on the age characteristics of platelets released into the blood in response to short‐term physical exercise, we conducted exercise experiments on 23 healthy subjects previously injected with different platelet populations labelled with 111In and 51Cr. Based on comparative platelet kinetic studies of pre‐ and post‐exercise platelets and on exercise experiments carried out while labelled‐platelet populations of different age composition were circulating, we cannot support the concept that the platelets released into the blood in response to exercise are enriched with young platelets. In addition, we found that simultaneously‐injected large and small platelets were released to the same extent in response to exercise, but that the sequestration patterns of large and small platelets seem to be different.

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