In vivo, label-free, three-dimensional quantitative imaging of kidney microcirculation using Doppler optical coherence tomography

Wierwille, Jeremiah; Andrews, Peter M.; Onozato, Maristela L.; Jiang, James; Cable, Alex; Chen, Yu

doi:10.1038/labinvest.2011.112

Cited by 37 publications

(45 citation statements)

References 72 publications

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“…Finally, OCT can be performed using a thin flexible sterile endoscope or catheter [26,27] or even with a needle [28], enabling ease of use for minimallyinvasive procedures and the possibility of imaging deep within a solid tissue or organ. Previously, we have demonstrated that OCT is able to provide clinically relevant information (morphology and blood flow) using a rat kidney model [29][30][31]. In addition, we showed that OCT has sufficient penetration depth and resolution to visualize human kidney microanatomy on intact human kidneys ex vivo even when surrounded by an intact connective tissue renal capsule [32,33].…”

Section: Imaging Donor Kidneys To Determine Their Statusmentioning

confidence: 82%

“…Blue-cyan represents blood flow in one direction while red-yellow represent blood flow in the opposite direction. In a previous publication, we have shown that DOCT will reveal glomerular blood flow and can be used to evaluate glomerular function/status [31]. …”

Section: In Vivo Oct Kidney Imagingmentioning

confidence: 99%

“…OCT image pixel dimensions were as follows: 1024 [X] by 512 [Z], and the associated 2D image scan dimensions were 3.75 mm [X] by 2.0 mm [Z]. The system's sensitivity was with ~90 dB [31].…”

Section: Oct System Setup and Designmentioning

confidence: 99%

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Using Optical Coherence Tomography (OCT) to Evaluate Human Donor Kidneys Prior to and Following Transplantation

Andrews

Chen²

2014

J Nephrol Ther

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The most common insult to donor kidneys destined for transplantation is Acute Tubular Necrosis (ATN). The extent of ATN will affect post-transplant function and is a significant risk factor for long-term graft function and survival. Optical Coherence Tomography (OCT) is a rapidly emerging imaging modality that can function as a type of "optical biopsy", providing non-invasive images of tissue morphology in situ and in real-time. In this paper, we review studies that support the use of OCT and Doppler based OCT (i.e., DOCT) to image the renal microstructure and blood flow of human donor kidneys. We conclude that OCT/DOCT imaging of donor kidneys prior to and following transplantation can provide transplant surgeons with a means for predicting ATN and post-transplant renal function. Imaging Donor Kidneys to Determine their StatusEnd-stage Renal Disease (ESRD) is associated with both high mortality rates and an enormous economic burden [1]. The preferred treatment option for ESRD that can extend patients' lives and improves their quality of life is kidney transplantation. However, ischemic insult suffered by kidneys awaiting transplantation frequently causes acute tubular necrosis (ATN) that leads to varying degrees of delayed graft function (DGF) after transplantation. Also, ATN represents a significant risk for eventual graft and patient survival [2,3], and can be difficult to discern from rejection. In present clinical practice, there is no reliable real-time test to determine the viability of donor kidneys and whether or not donor kidneys might exhibit ATN. Therefore, there is a critical need for objective and reliable real-time tests to predict ATN to use these organs safely and utilize the donor pool optimally.Previously it has been shown that the non-invasive imaging techniques (i.e., tandem scanning confocal microscopy-TSCM) could be used to determine the degree of ATN by analyzing the superficial nephrons of living kidneys in animal models and that these observations correlate with post-transplant renal function [4,5]. This is not surprising in that the status of superficial proximal convoluted tubules is indicative of the status of proximal convoluted throughout the entire kidney cortex. Non-invasive microscopic techniques are necessary for this determination because conventional microscopy results in artifacts that are difficult to distinguish from ATN [6]. Other investigators have also used near-infrared confocal microscopy [7] and multi-photon microscopy (MPM) [8][9][10] to demonstrate the ability to perform non-invasive imaging of kidney structure and function in animal models. However, the maximum penetration depth of those techniques for kidney imaging is very limited (≈100 µm for TSCM, and ≈200-300 µm for MPM), which makes it difficult to impossible to nondestructively image the human kidney, especially if an intact human renal capsule surrounds it. Indeed, in a previous clinical trial, we found that the limited penetrating ability of TSCM precluded us from imaging human donor kidneys even...

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Section: Imaging Donor Kidneys To Determine Their Statusmentioning

confidence: 82%

Section: In Vivo Oct Kidney Imagingmentioning

confidence: 99%

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Using Optical Coherence Tomography (OCT) to Evaluate Human Donor Kidneys Prior to and Following Transplantation

Andrews

Chen²

2014

J Nephrol Ther

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“…Since then, DOCT has had great success in the visualisation of ocular media and related flow dynamics (see Figure 26) [346][347][348][349], in addition to cerebral [350], cardiovascular [351] and urological [352] applications.…”

Section: Dopplermentioning

confidence: 99%

‘Go with the flow ’: A review of methods and advancements in blood flow imaging

Daly

Leahy

2012

Journal of Biophotonics

108

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Physics has delivered extraordinary developments in almost every facet of modern life. From the humble thermometer and stethoscope to X‐Ray, CT, MRI, ultrasound, PET and radiotherapy, our health has been transformed by these advances yielding both morphological and functional metrics. Recently high resolution label‐free imaging of the microcirculation at clinically relevant depths has become available in the research domain. In this paper, we present a comprehensive review on current imaging techniques, state‐of‐the‐art advancements and applications, and general perspectives on the prospects for these modalities in the clinical realm. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…OCT is a high-resolution, cross-sectional, three-dimensional imaging modality [1][2][3][4][5][6][7][8]. We have studied OCT system and its application for over ten years [9][10], and have accumulated the enough knowledge for teaching.…”

Section: ．Introductionmentioning

confidence: 99%

Reform and practice of optical coherence tomography (OCT) system-driven teaching for optoelectronic instrument principle and design

2017

14th Conference on Education and Training in Optics and Photonics: ETOP 2017

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In vivo, label-free, three-dimensional quantitative imaging of kidney microcirculation using Doppler optical coherence tomography

Cited by 37 publications

References 72 publications

Using Optical Coherence Tomography (OCT) to Evaluate Human Donor Kidneys Prior to and Following Transplantation

Using Optical Coherence Tomography (OCT) to Evaluate Human Donor Kidneys Prior to and Following Transplantation

‘Go with the flow ’: A review of methods and advancements in blood flow imaging

Reform and practice of optical coherence tomography (OCT) system-driven teaching for optoelectronic instrument principle and design

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