In vivo microdialysis and reverse phase ion pair liquid chromatography/tandem mass spectrometry for the determination and identification of acetylcholine and related compounds in rat brain

Zhang, Yongxin; Wong, Philip; Cregor, Meloney; Gitzen, James F.; Coury, Louis A.; Kissinger, Peter T.

doi:10.1002/1097-0231(20000930)14:18<1695::aid-rcm79>3.0.co;2-5

Cited by 79 publications

(84 citation statements)

References 19 publications

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“…ACh has been detected by various methods, such as liquid chromatography/electrochemical (LC-EC) method [36][37][38], liquid chromatography/electrospray ionization/ mass spectrometry (LC-ESI-MS) [8,[39][40][41][42], liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry (LC-APCI-MS) [43], as well as MALDI-TOF-MS with CHCA as the matrix [12]. The performances of these methods have been listed in detail on Table 3.…”

Section: Quantitative Methods Validation and Calibration Curvesmentioning

confidence: 99%

High Throughput Enzyme Inhibitor Screening by Functionalized Magnetic Carbonaceous Microspheres and Graphene Oxide-Based MALDI-TOF-MS

Liu

et al. 2011

J. Am. Soc. Mass Spectrom.

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In this work, a high throughput methodology for screening enzyme inhibitors has been demonstrated by combining enzyme immobilized magnetic carbonaceous microspheres and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with grapheme oxide as matrix. First, model enzyme acetylcholinesterase (AChE) was immobilized onto the 3-glycidoxypropyltrimethoxysilane (GLYMO)-modified magnetic carbonaceous (MC) microspheres, displaying a high enzyme activity and stability, and also facilitating the separation of enzyme from substrate and product. The efficiency of immobilized AChE was monitored by biochemical assay, which was carried out by mixing enzyme-immobilized MC microspheres with model substrate acetylcholine (ACh), and subsequent quantitative determination of substrate ACh and product choline using graphene oxide-based MALDI-TOF-MS with no background inference. The limit of detection (LOD) for ACh was 0.25 fmol/μL, and excellent linearity (R 2 =0.9998) was maintained over the range of 0.5 and 250 fmol/μL. Choline was quantified over the range of 0.05 and 15 pmol/μL, also with excellent linearity (R 2 =0.9994) and low LOD (0.15 fmol/μL). Good accuracy and precision were obtained for all concentrations within the range of the standard curves. All together, eight compounds (four known AChE inhibitors and four control chemical compounds with no AChE inhibit effect) were tested with our promoted methodology, and the obtained results demonstrated that our high throughput screening methodology could be a great help to the routine enzyme inhibitor screening.

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Section: Quantitative Methods Validation and Calibration Curvesmentioning

confidence: 99%

High Throughput Enzyme Inhibitor Screening by Functionalized Magnetic Carbonaceous Microspheres and Graphene Oxide-Based MALDI-TOF-MS

Liu

et al. 2011

J. Am. Soc. Mass Spectrom.

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“…LC/MSMS has been reported by several groups. 6,7 2·2 Automated blood sampling Automation of in vivo techniques has not kept pace with in vitro techniques, or with the analytical methods used to process biological samples after collection. Serial blood sampling from rodents can be a logistical challenge when conducted by hand over 12 -48 h. The prevailing methodology for manual blood sampling uses basic tools such as syringes and devices for severely restraining animals for tail vein puncture.…”

Section: ·1 Membrane Sampling Probesmentioning

confidence: 99%

Good Preclinical Bioanalytical Chemistry Requires Proper Sampling from Laboratory Animals: Automation of Blood and Microdialysis Sampling Improves the Productivity of LC/MSMS.

et al. 2003

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“…This hydrophobicity diversity poses challenges for the LC-MS analysis. Currently, the separation of QACs of high polarity, such as choline, acetylcholine, carnitine and acetylcarnitine, are commonly conducted using hydrophilic interaction chromatography (HILIC)-MS [13,14], ion-exchange chromatography [15,16], or ionpairing reversed phase (RP) chromatography [17][18][19]. To separate carnitines of both low and high polarities, pre-column derivatization has been used to improve their separation on common RP columns [20].…”

Section: Introductionmentioning

confidence: 99%

Analysis of multiple quaternary ammonium compounds in the brain using tandem capillary column separation and high resolution mass spectrometric detection

Falasca

Petruzziello

Kretz

et al. 2012

Journal of Chromatography A

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In vivo microdialysis and reverse phase ion pair liquid chromatography/tandem mass spectrometry for the determination and identification of acetylcholine and related compounds in rat brain

Cited by 79 publications

References 19 publications

High Throughput Enzyme Inhibitor Screening by Functionalized Magnetic Carbonaceous Microspheres and Graphene Oxide-Based MALDI-TOF-MS

High Throughput Enzyme Inhibitor Screening by Functionalized Magnetic Carbonaceous Microspheres and Graphene Oxide-Based MALDI-TOF-MS

Good Preclinical Bioanalytical Chemistry Requires Proper Sampling from Laboratory Animals: Automation of Blood and Microdialysis Sampling Improves the Productivity of LC/MSMS.

Analysis of multiple quaternary ammonium compounds in the brain using tandem capillary column separation and high resolution mass spectrometric detection

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