In vivo models of proliferative vitreoretinopathy

Abstract

We outline current in vitro and in vivo models for experimental proliferative vitreoretinopathy (PVR) and provide a detailed protocol of our standardized in vivo PVR model. PVR is the leading cause of failed surgical procedures for the correction of rhegmatogenous retinal detachment. The pathogenesis of this multifactorial condition is still not completely understood. Experimental models for PVR help us understand the factors that play a role in the pathogenesis of the disease process in a controlled manner and allow for reproducible preclinical assessment of novel therapeutic interventions. We describe a cell injection model in detail that uses homologous retinal pigment epithelial (RPE) cell cultures to induce PVR over a 2-8 week period.

αB-crystallin regulation of angiogenesis by modulation of VEGF

“…21 Intravitreal injection was performed as previously reported. 16,20,22,23 HSPB2 expression in posterior eye cups HSPB2 gene expression in normal posterior eye cups (retina, RPE, choroid) from p17 and adult mice and fetal human eyes and posterior eye cups from mice at P12, P17 in OIR and day 4 and day 7 after laser were analyzed by RT-PCR. As positive controls, muscle tissue (human fetal and murine adult) and heart tissue (murine adult) also were evaluated.…”

Role of Chlamydia trachomatis intraocular infection in the development of proliferative vitreoretinopathy (experimental study)

“…В частности, применяют введение культу-ры фибробластов кожи, аутологичных клеток пиг-ментного эпителия сетчатки, плазмы с тромбоцита-ми, мононуклеаров крови, ферментов, избиратель-но разрушающих коллагены базальных мембран (диспаза), а также проведение ретинотомии с витр-эктомией, криотерапии [4][5][6][7][8]. Как в случае есте-ственного течения ПВР, эти модели основаны на появлении в стекловидном теле пула клеток с про-лиферативным потенциалом.…”

Tumor Necrosis Factor-α Regulates Transforming Growth Factor-β-dependent Epithelial-Mesenchymal Transition by Promoting Hyaluronan-CD44-Moesin Interaction

“…The PVR membrane consists of extracellular matrix, retinal pigment epithelium (RPE), retinal glial cells, fibroblasts, and inflammatory macrophages (23,24). Intravitreal cell injection models of PVR show that not only fibroblasts, but also RPE cells, are associated with the formation of intraocular membrane (25). Various growth factors and cytokines, which are inflammatory products of cell activation, were increased in vitreous aspirates from the eyes with PVR.…”