In vivo molecular imaging of epidermal growth factor receptor in patients with colorectal neoplasia using confocal laser endomicroscopy

Endomicroscopy in Barrett's Esophagus

“…An anti-EGFR antibody has been developed that binds to colonic neoplasia, but no targeting antibodies have been developed for BE neoplasia. 32 A small fluorescent-labeled peptide specific for neoplastic BE has also been developed and tested in EMR specimens. 33 The fluorescence intensity was measured for each specimen and was found to be highest in neoplastic BE tissue and lowest in squamous and gastric mucosa.…”

From bench to bedside

“…An EGFR-specific fluorescence signal was present in 18/19 CRC patients and 12/18 patients with intestinal adenomas, whereas only weak signals were found in normal mucosa. Importantly, no antibody formation against the labeled probe was found, although this was tested in only four patients [27]. A major breakthrough was the recent first-in-human pilot study where a cyclic peptide conjugated to a fluorescent cyanine dye that binds the human tyrosine kinase hepatocyte growth factor receptor (c-Met) was i.v.…”

Imaging the Leaky Gut

“…[1][2][3][4] Through the use of vascular and molecular contrast agents, multiple structural and functional events can be directly observed, including angiogenesis and monoclonal antibody-antigen binding to epithelial targets. 5 In aggregate, these technologies have opened new fields of endomicroscopy and functional imaging of the GI tract at cellular and subcellular level.…”

“…10 Molecular imaging with tagged monoclonal antibody has enabled imaging of antiendothelial growth factor receptor monoclonal antibody binding to targets and predication of response to endothelial growth factor receptor chemotherapy. 5,9,11 Imaging of overt and microscopic inflammation is now well established. Kiesslich et al 9,11 demonstrated epithelial cell shedding in the small bowel of patients with inflammatory bowel disease and further demonstrated that residual inflammation was predictive of relapse.…”

“…For example, the protective effect of coffee consumption for HCC risk 4 motivated a cohort study that identified an inverse association between consumption of polyphenol compounds contained in coffee and HCC incidence. 5 Although the search for bioactive nutrients can lead to the development of preventive measures and potential treatments, proponents of whole food and dietary pattern analysis counter that this approach lacks context because these compounds are not consumed in isolation. 6 Instead, people tend to eat in dietary patterns, ranging from the healthy fat and plant-rich Mediterranean diet to the "meat-sweet" diet of developed countries heavy in processed foods, refined sugars, and red meat.…”