In vivo monitoring and high-resolution characterizing of the prednisolone-induced osteoporotic process on adult zebrafish by optical coherence tomography

Lin, Yunting; Xiang, Xiang; Chen, Tingru; Gao, Chudan; Fu, Hong-Bo; Wang, Limei; Deng, Lijun; Zeng, Lvming; Zhang, Jian

doi:10.1364/boe.10.001184

Cited by 21 publications

(17 citation statements)

References 40 publications

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“…Our data reconfirm the reliability of adult zebrafish caudal fin to be used as a reliable model to verify glucocorticoid-induced osteoporosis. (2) In 2016, Cardeira et al (20) suggested analysing the caudal fin de novo mineralization by calculating the parameters (RMA, RAY, REG and STU) of many zebrafish individuals during caudal fin regeneration. Here, the drugs' after-effects in the lepidotrichia could be clearly observed with the methodology proposed.…”

Section: Discussionmentioning

confidence: 99%

“…(1) The increased bone remodelling activity in favour of osteoclasts often leads to reduced bone-mineral density (BMD), high porosity and, consequently, in the deterioration of mechanical properties and high fracture risk. (1)(2)(3) As the people's life expectancy increases, (4) osteoporosis may cause a significant impact on the life quality of elderly people and burden the world's economy in a short-time. (2,5) Prednisolone (PN) is a glucocorticoid often used as a treatment to inflammation and its prolonged administration is well known to cause secondary osteoporosis in humans.…”

Section: Introductionmentioning

confidence: 99%

“…(1)(2)(3) As the people's life expectancy increases, (4) osteoporosis may cause a significant impact on the life quality of elderly people and burden the world's economy in a short-time. (2,5) Prednisolone (PN) is a glucocorticoid often used as a treatment to inflammation and its prolonged administration is well known to cause secondary osteoporosis in humans. (6) In order to counteract the adverse effects of the continuous use of glucocorticoids, antiresorptive agents (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Prednisolone and Alendronate on the de novo Mineralization of Zebrafish Caudal Fin

et al. 2020

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Dysregulated balance between bone resorption and formation mediates the onset and progression of osteoporosis. The administration of prednisolone is known to induce osteoporosis, whereas alendronate is commonly used to reverse the process. However, the assessment of the effects of such medicines on the nanostructure of bone remodeling and mechanical properties remains a major technical challenge. The aim of this study was to apply various analytical approaches to evaluate the compositional, morphological, and mechanical properties of regenerative zebrafish caudal fin bony rays affected by prednisolone and alendronate. Adult wild‐type AB strain zebrafish were first exposed to 125μM of prednisolone for 14 days to develop glucocorticoid‐induced osteoporosis. Fish fins were then amputated and let to regenerate for 21 days in tank water containing 30μM of alendronate or no alendronate. The lepidotrichia in the proximal and distal regions were evaluated separately using confocal microscope, scanning electron microscope, electron‐dispersive spectroscopy, Raman spectroscopy, atomic force microscopy, and a triboindenter. As expected, prednisolone led to significant osteoporotic phenotypes. A decrease of Ca/P ratio was observed in the osteoporotic subjects (1.46 ± 0.04) as compared to the controls (1.74 ± 0.10). Subsequent treatment of alendronate overmineralized the bony rays during regeneration. Enhanced phosphate deposition was detected in the proximal part with alendronate treatment. Moreover, prednisolone attenuated the reduced elastic modulus and hardness levels (5.60 ± 5.04 GPa and 0.12 ± 0.17 GPa, respectively), whereas alendronate recovered them to the pre‐amputation condition (8.68 ± 8.74 GPa and 0.34 ± 0.47 GPa, respectively). As an emerging model of osteoporosis, regrowth of zebrafish caudal fin was shown to be a reliable assay system to investigate the various effects of medicines in the de novo mineralization process. © 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Prednisolone and Alendronate on the de novo Mineralization of Zebrafish Caudal Fin

et al. 2020

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“…In vivo high-resolution images on adult zebrafish skeleton can be also obtained with spectral-domain optical coherence tomography (SD-OCT). Recently, bone defects due to a prednisolone treatment have been quantitatively evaluated using the SD-OCT images at different time points during a period of 21 days [121].…”

Section: Adult Zebrafishmentioning

confidence: 99%

Zebrafish Models of Human Skeletal Disorders: Embryo and Adult Swimming Together

Carnovali

Banfi

Mariotti

2019

BioMed Research International

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Danio rerio (zebrafish) is an elective model organism for the study of vertebrate development because of its high degree of homology with human genes and organs, including bone. Zebrafish embryos, because of the optical clarity, small size, and fast development, can be easily used in large-scale mutagenesis experiments to isolate mutants with developmental skeletal defects and in high-throughput screenings to find new chemical compounds for the ability to revert the pathological phenotype. On the other hand, the adult zebrafish represents another powerful resource for pathogenic and therapeutic studies about adult human bone diseases. In fish, some characteristics such as bone turnover, reparation, and remodeling of the adult bone tissue cannot be found at the embryonic stage. Several pathological models have been established in adult zebrafish such as bone injury models, osteoporosis, and genetic diseases such as osteogenesis imperfecta. Given the growing interest for metabolic diseases and their complications, adult zebrafish models of type 2 diabetes and obesity have been recently generated and analyzed for bone complications using scales as model system. Interestingly, an osteoporosis-like phenotype has been found to be associated with metabolic alterations suggesting that bone complications share the same mechanisms in humans and fish. Embryo and adult represent powerful resources in rapid development to study bone physiology and pathology from different points of view.

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“…Furthermore, SD‐OCT could accurately assess brain damage in adult zebrafish and monitor the repairing process after injury. Recently, dynamic monitoring and evaluation of the prednisolone‐induced osteoporosis process were obtained by our SD‐OCT that will be a very useful approach for the basic research of osteoporosis .…”

Section: Introductionmentioning

confidence: 99%

In vivo monitoring the dynamic process of acute retinal hemorrhage and repair in zebrafish with spectral‐domain optical coherence tomography

Lin

Xiang

Chen

et al. 2019

Journal of Biophotonics

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Retina, the only light sensor in the human eye, is hidden and extremely fragile. Optimized animal models and efficient imaging techniques are very important for the study of retinopathy. In this work, the rapid retinal injury process and the long‐term retinal repair process were in vivo continuously evaluated with a novel imaging technology spectral‐domain optical coherence tomography (SD‐OCT) in a unique animal model zebrafish. Acute retinal injury was constructed on adult zebrafish by needle injection surgery. SD‐OCT imaging was carried out immediately after the mechanical injury. The retinal hemorrhage, which lasted only 5 seconds, could be visualized dynamically by SD‐OCT. The process of blood clearance and retinal repair was also evaluated because SD‐OCT imaging is nondestructive. Both SD‐OCT imaging results and behavioral analyzing results demonstrated that zebrafish retina could be repaired by itself within 15 days, which was confirmed by the results of pathological experiment.

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In vivo monitoring and high-resolution characterizing of the prednisolone-induced osteoporotic process on adult zebrafish by optical coherence tomography

Cited by 21 publications

References 40 publications

Influence of Prednisolone and Alendronate on the de novo Mineralization of Zebrafish Caudal Fin

Influence of Prednisolone and Alendronate on the de novo Mineralization of Zebrafish Caudal Fin

Zebrafish Models of Human Skeletal Disorders: Embryo and Adult Swimming Together

In vivo monitoring the dynamic process of acute retinal hemorrhage and repair in zebrafish with spectral‐domain optical coherence tomography

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