In vivo monitoring of amino acids by microdialysis sampling with on-line derivatization by naphthalene-2,3-dicarboxyaldehyde and rapid micellar electrokinetic capillary chromatography

Shou, Minshan; Smith, Anthony D.; Shackman, Jonathan G.; Peris, Joanna; Kennedy, Robert T.

doi:10.1016/j.jneumeth.2004.04.006

Cited by 68 publications

(58 citation statements)

References 39 publications

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“…Different means for sample manipulations in the mTAS, such as sample introduction to the chip, preseparation, derivatization, microdispensing, microinjection (electrokinetic, pressurized, piezoelectric, optical gating, microrotary valves), mixing by static mixers, centrifugal force, capillary force, pneumatic propulsion, and electromanipulation, are discussed in the recent review [63]. These techniques are particularly useful for handling of small sample volumes, such as in single cell analysis [64] and in the analysis of microbiopsy and microdialysis samples [65].…”

Section: Micromanipulationmentioning

confidence: 99%

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Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Micromanipulationmentioning

confidence: 99%

“…For in vivo monitoring of biomolecules in body fluids and tissues, e.g., neurotransmitters in the brain, microdialysis cells composed of semipermeable membrane, typically 1-4 mm long and 0.2-0.4 mm in diameter, are fashioned into a probe, which is implanted in the brain or other tissue [65].…”

Section: Micromanipulationmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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“…A very near on-line derivatization system was described by Shou et al (2004) and Smith et al (2004). Microdialysate was continuously derivatized on-line by mixing 6 mM NDA and 10 mM potassium cyanide with the dialysate stream in a fused silica capillary to form the fluorescent products.…”

Section: Amino Acidsmentioning

confidence: 99%

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

Rammouz

Lacroix

Garrigues

et al. 2007

Biomedical Chromatography

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This paper reviews analytical methods, instrumental developments and applications for derivatization of primary amines with naphthalene-2,3-dicarboxaldehyde using fluorescence and chemiluminescence detection with capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). The use of lasers as well as lamps as the excitation source for fluorescence detection is discussed. The detection limit observed with naphthalene-2,3-dicarboxaldehyde derivatization is often lower and better than those obtained with other analytical separations and other fluorescent dyes. In addition, this paper describes the crucial points that influence the stability of NDA primary amine derivatives, and summarize the separation, derivatization and migration conditions of the different techniques, with their advantages and drawbacks.

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“…In all cases, molecules were tagged with NDA. 30 mM SDS in 15 mM phosphate buffer, at pH 8.0, was the electrolyte used by Shou et al [104] to monitor 17 amino acids collected by in vivo microdialysis. Microdialysate was derivatized on-line by mixing the dialysate stream with 6 mM NDA and 10 mM potassium cyanide.…”

Section: Amino Acids In Microdialysatesmentioning

confidence: 99%

Determination of amino acids by micellar EKC: Recent advances in method development and novel applications to different matrices

et al. 2007

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The extensive use of CE for the analysis of amino acids has been well documented in a series of research articles and reviews. Aim of this report is to address the attention of the reader on the recent advances of micellar electrokinetic chromatography for the separation and determination of these analytes. Enhancements in selectivity of this technique through the use of pseudostationary phases containing mixed micelles, polymers, and chiral selectors are presented. Selected applications concerning separation and quantitation of even minute amounts of amino acids in: (i) biological fluids; (ii) microdialysates; (iii) plant cells; (iv) food stuff; and (v) pharmaceutical formulations have also been covered. Advantages of MEKC over other techniques for the amino acid analysis have been underlined.

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In vivo monitoring of amino acids by microdialysis sampling with on-line derivatization by naphthalene-2,3-dicarboxyaldehyde and rapid micellar electrokinetic capillary chromatography

Cited by 68 publications

References 39 publications

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

Determination of amino acids by micellar EKC: Recent advances in method development and novel applications to different matrices

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