In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb

Kovalchuk, Yury; Homma, Ryota; Liang, Yajie; Maslyukov, Anatoliy; Hermes, Marina; Thestrup, Thomas; Griesbeck, Oliver; Ninkovic, Jovica; Cohen, Lawrence B.; Garaschuk, Olga

doi:10.1038/ncomms7349

Cited by 30 publications

(57 citation statements)

References 64 publications

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“…This period of time, which we named "pre-integration phase", is characterized by a vigorous migratory behavior of adult-born cells, long-distance lateral migration of JGNs with characteristic temporal (stop and go) and spatial (migratory, unidirectional or multidirectional) patterns and odor responsiveness [20] of migrating cells. Lasting for 3-4 weeks, pre-integration phase is the time period of initial fate determination of adult-born cells, critically impacting on the settlement location of cells, their odor responsiveness (for JGNs predetermined by the glomeruli in which they integrate [37]) and likely also their survival rate.…”

Section: Discussionmentioning

confidence: 99%

“…During the early pre-integration phase, the migratory behavior of adultborn cells is stereotypic and is likely to be driven by the molecular clues [18,38]. Only a few days later, however, migrating JGNs (and likely also GCs) become responsive to sensory stimuli [20]. From this point forward, the migration of adult-born JGNs is likely to be driven both by extrinsic sensory stimuli and by intrinsic molecular clues, some of which are influenced by sensory stimuli [18,39].…”

Section: Discussionmentioning

confidence: 99%

“…Examination of a few fluorescent dyes led us to choose sulforhodamine B, which can be effectively excited by two-photon laser and can be cleared from the body in a few hours, allowing enough acquisition time [20] while minimizing the potential side effects, if there are any.…”

Section: The Use Of Ocps For Long-term In Vivo Tracking Of Individualmentioning

confidence: 99%

“…To evaluate the effectiveness of oCPS for tracking of adult-born cells in the OB, the mixture of the RGB retroviral vectors was injected into the RMS (Figure 2A), where adult-born cells are actively dividing [18,20,25]. Stochastic transduction of cells by the three retroviruses labeled them in one of seven easily distinguishable colors (three basic colors: red, green blue; four mixed colors: yellow, cyan, violet and white; Figure 2B and 2C), whereas angiography allowed us to identify the position of each RGB-marked cells ( Figure 2B, right).…”

Section: Ocps Reveals An Active Migration Of Adult-born Neurons In Thmentioning

confidence: 99%

“…In our previous work, we also observed a displacement of cell bodies of JGNs during a 4-h-long ob-servation time window (e.g., Figure 2a in ref. [20]), but were unable to perform long-term single-cell tracking in acute in vivo experiments.…”

Section: Introductionmentioning

confidence: 99%

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Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb

Liang

Riecken

et al. 2016

Cell Res

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The behavior of adult-born cells can be easily monitored in cell culture or in lower model organisms, but longitudinal observation of individual mammalian adult-born cells in their native microenvironment still proves to be a challenge. Here we have established an approach named optical cell positioning system for long-term in vivo single-cell tracking, which integrates red-green-blue cell labeling with repeated angiography. By combining this approach with in vivo two-photon imaging technique, we characterized the in vivo migration patterns of adult-born neurons in the olfactory bulb. In contrast to the traditional view of mere radial migration of adult-born cells within the bulb, we found that juxtaglomerular cells switch from radial migration to long distance lateral migration upon arrival in their destination layer. This unique long-distance lateral migration has characteristic temporal (stop-and-go) and spatial (migratory, unidirectional or multidirectional) patterns, with a clear cell age-dependent decrease in the migration speed. The active migration of adult-born cells coincides with the time period of initial fate determination and is likely to impact on the integration sites of adult-born cells, their odor responsiveness, as well as their survival rate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Use Of Ocps For Long-term In Vivo Tracking Of Individualmentioning

confidence: 99%

Section: Ocps Reveals An Active Migration Of Adult-born Neurons In Thmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb

Liang

Riecken

et al. 2016

Cell Res

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Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb

Figarella

et al. 2023

Cell. Mol. Life Sci.

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The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca2+ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K+ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca2+ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression. Moreover, K+ channel overexpression strongly downregulated genes involved in neuronal migration, differentiation, and morphogenesis and upregulated apoptosis-related genes, thus locking adult-born cells in an immature and vulnerable state. Surprisingly, cells deprived of sensory-driven activity developed normally. Together, the data reveal signaling pathways connecting the endogenous intermittent neuronal activity/Ca2+ fluctuations as well as enhanced Kv1.2/Kir2.1 K+ channel function to migration, maturation, and survival of adult-born neurons.

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Current Understanding of the Pathways Involved in Adult Stem and Progenitor Cell Migration for Tissue Homeostasis and Repair

Goichberg

2016

Stem Cell Rev and Rep

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With the advancements in the field of adult stem and progenitor cells grows the recognition that the motility of primitive cells is a pivotal aspect of their functionality. There is accumulating evidence that the recruitment of tissue-resident and circulating cells is critical for organ homeostasis and effective injury responses, whereas the pathobiology of degenerative diseases, neoplasm and aging, might be rooted in the altered ability of immature cells to migrate. Furthermore, understanding the biological machinery determining the translocation patterns of tissue progenitors is of great relevance for the emerging methodologies for cell-based therapies and regenerative medicine. The present article provides an overview of studies addressing the physiological significance and diverse modes of stem and progenitor cell trafficking in adult mammalian organs, discusses the major microenvironmental cues regulating cell migration, and describes the implementation of live imaging approaches for the exploration of stem cell movement in tissues and the factors dictating the motility of endogenous and transplanted cells with regenerative potential.

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In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb

Cited by 30 publications

References 64 publications

Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb

Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb

Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb

Current Understanding of the Pathways Involved in Adult Stem and Progenitor Cell Migration for Tissue Homeostasis and Repair

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