In-vivo optical detection of cancer using chlorin e6 – polyvinylpyrrolidone induced fluorescence imaging and spectroscopy

Chin, William W.; Thong, Patricia S. P.; Ramaswamy, Bhuvaneswari; Soo, Khee Chee; Heng, Paul Wan Sia; Olivo, Malini

doi:10.1186/1471-2342-9-1

Cited by 37 publications

(19 citation statements)

References 27 publications

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“…Soon after, important work elaborating on the MPI signal equations and 3D in vivo applications of the system matrix method were developed (Rahmer et al 2009; J Weizenecker et al 2009) as well as the description of the x-space approach to MPI (Goodwill and Conolly 2010; Goodwill and Conolly 2011; Saritas et al 2013). MPI uses magnetic fields to detect superparamagnetic iron oxide (SPIO) tracers present in an imaging field of view (FOV).…”

Section: Introductionmentioning

confidence: 99%

“…Lu et al 2013; Konkle, Goodwill, Hensley, et al 2015). Reconstruction may also be formulated as an inverse problem by measuring system functions and constructing a suitable system matrix (Rahmer et al 2009; Rahmer et al 2012). Per the saturation physics described in Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Per the saturation physics described in Fig. 1, both of these reconstruction methods report resolution that scales inversely with the gradient strength (Rahmer et al 2009; Goodwill and Conolly 2010). …”

Section: Introductionmentioning

confidence: 99%

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Combining magnetic particle imaging and magnetic fluid hyperthermia in a theranostic platform

et al. 2017

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Magnetic particle imaging (MPI) is a rapidly developing molecular and cellular imaging modality. Magnetic fluid hyperthermia (MFH) is a promising therapeutic approach where magnetic nanoparticles are used as a conduit for targeted energy deposition, such as in hyperthermia induction and drug delivery. The physics germane to and exploited by MPI and MFH are similar, and the same particles can be used effectively for both. Consequently, the method of signal localization by using gradient fields in MPI can also be used to spatially localize MFH, allowing for spatially selective heating deep in the body and generally providing greater control and flexibility in MFH. Furthermore, MPI and MFH may be integrated together in a single device for simultaneous MPI-MFH and seamless switching between imaging and therapeutic modes. Here we show simulation and experimental work quantifying the extent of spatial localization of MFH using MPI systems: We report the first combined MPI-MFH system and demonstrate on-demand selective heating of nanoparticle samples separated by only 3 mm (up to 0.4 C/s heating rates and 150 W/g SAR deposition). We also show experimental data for MPI performed at a typical MFH frequency and show preliminary simultaneous MPI-MFH data.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Combining magnetic particle imaging and magnetic fluid hyperthermia in a theranostic platform

et al. 2017

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“…Chlorins are particularly strong under investigations in preclinical and clinical fluorescence diagnostics and photodynamic therapy of bladder tumors. Formulation of chlorin e6 in combination with the hydrophilic polyvinylpyrrolidone exhibited in vitro and in vivo high fluorescence intensity and selective phototoxicity against bladder cancer [11,13,47]. Ce6-PVP showed efficacy and safety for use in patients with bladder cancer.…”

Section: Discussionmentioning

confidence: 99%

Chlorin-based photodynamic therapy enhances the effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in bladder cancer cells

et al. 2012

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SummaryBackgroundPhotodynamic therapy (PDT) is an attractive, emerging therapeutic procedure suitable for the treatment of non-muscle-invasive bladder cancer. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a death ligand that belongs to the TNF superfamily of cytokines. The ability of TRAIL to selectively induce apoptosis in cancer cells but not in normal cells promotes the development of TRAIL-based cancer therapy. However, many tumor cells are resistant to TRAIL-induced apoptosis. The purpose of the study was to overcome TRAIL-resistance in bladder cancer cells by photodynamic therapy (PDT).Material/MethodsThree human bladder transitional cancer cell lines – T24, 647V and SW780 – were treated with TRAIL and/or chlorin-based PDT. The cytotoxicity was measured by MTT and LDH assays and apoptosis was detected using annexin V by flow cytometry.ResultsOur test confirmed that T24 and 647V bladder cancer cells are resistant to TRAIL, whereas SW780 cells are sensitive to TRAIL. Then we examined the cytotoxic and apoptotic effects of TRAIL in combination with chlorin e6-polyvinylpyrrolidone (Ce6-PVP)-mediated PDT on bladder cancer cells. We showed for the first time that pretreatment with a low dose of PDT significantly sensitizes bladder cancer cells to TRAIL-induced apoptosis. Chlorin-based PDT augments the effect of TRAIL on bladder cancer cells.ConclusionsPDT with Ce6-PVP photosensitizer enhances the cytotoxic and apoptotic effects of TRAIL on bladder cancer cells. The obtained results suggest that combined treatment by TRAIL and PDT may provide the basis for a new therapeutic approach to induce cell death in bladder cancer.

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“…These results demonstrated the high potential of Ce6‐PVP being used as a fluorescent marker for cancer diagnosis and therapy monitoring. Thus, more recently, they also performed fluorescence imaging and spectroscopy on animal models and clinical patients with angiosarcoma . Since Ce6‐PVP fluoresces as red spots upon blue‐light excitation, the red‐to‐blue fluorescence intensity contrast ratio was used as an image parameter to differentiate Ce6‐PVP uptake between tumor and normal cells.…”

Section: Fluorescent Techniquesmentioning

confidence: 99%

Advances in fluorescence diagnosis to track footprints of cancer progression in vivo

Olivo

2013

Laser & Photonics Reviews

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Fluorescence spectroscopy and imaging have been widely used for in vivo cancer diagnosis and therapy monitoring in preclinical models, as well as clinical translation. Great attempts have been made to develop novel fluorescence techniques and improve on existing ones, which can now be used in conjunction with newly developed fluorescent probes for specific cancer imaging. In this review, a broad overview of fluorescence techniques is provided, including photodynamic diagnosis, laser confocal endomicroscopy and fluorescence lifetime imaging, coupled with endogenous and exogenous fluorophores. In particular, endogenous fluorophores, such as nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), are highlighted as they are linked to cellular metabolism in precancer growth. The use of near-infrared dyes, such as indocynanine green (ICG), for imaging deep-tissue regions is also reviewed. In addition, diagnostic algorithms used for tissue classification and cancer detection will be discussed. Lastly, emerging technologies in fluorescence diagnosis will also be included.

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In-vivo optical detection of cancer using chlorin e6 – polyvinylpyrrolidone induced fluorescence imaging and spectroscopy

Cited by 37 publications

References 27 publications

Combining magnetic particle imaging and magnetic fluid hyperthermia in a theranostic platform

Combining magnetic particle imaging and magnetic fluid hyperthermia in a theranostic platform

Chlorin-based photodynamic therapy enhances the effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in bladder cancer cells

Advances in fluorescence diagnosis to track footprints of cancer progression in vivo

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