In Vivo Paracoccidioides sp. Biofilm on Vascular Prosthesis

Cattana, María Emilia; Tracogna, María F.; Marques, I. C. S.; Rojas, Florencia; Fernández, Mariana Soledad; Sosa, María de los Ángeles; Mussin, Javier Esteban; Giusiano, Gustavo

doi:10.1007/s11046-017-0126-8

Cited by 8 publications

(9 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both fungi may grow as in vitro or in vivo mono-species biofilms (Hawser and Douglas, 1994;Douglas, 2003;Sardi Jde et al, 2015;Cattana et al, 2017). Therefore, evaluating the dynamics of mono-and dual-species biofilm formed by P. brasiliensis and C. albicans can widen the knowledge about the PCM infection mechanism, the most important mycotic disease in Latin America.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

et al. 2020

View full text Add to dashboard Cite

The oral cavity is a highly diverse microbial environment in which microorganisms interact with each other, growing as biofilms on biotic and abiotic surfaces. Understanding the interaction among oral microbiota counterparts is pivotal for clarifying the pathogenesis of oral diseases. Candida spp. is one of the most abundant fungi in the oral mycobiome with the ability to cause severe soft tissue lesions under certain conditions. Paracoccidioides spp., the causative agent of paracoccidioidomycosis, may also colonize the oral cavity leading to soft tissue damage. It was hypothesized that both fungi can interact with each other, increasing the growth of the biofilm and its virulence, which in turn can lead to a more aggressive infectivity. Therefore, this study aimed to evaluate the dynamics of mono-and dual-species biofilm growth of Paracoccidioides brasiliensis and Candida albicans and their infectivity using the Galleria mellonella model. Biomass and fungi metabolic activity were determined by the crystal violet and the tetrazolium salt reduction tests (XTT), respectively, and the colony-forming unit (CFU) was obtained by plating. Biofilm structure was characterized by both scanning electronic-and confocal laser scanning-microscopy techniques. Survival analysis of G. mellonella was evaluated to assess infectivity. Our results showed that dual-species biofilm with P. brasiliensis plus C. albicans presented a higher biomass, higher metabolic activity and CFU than their mono-species biofilms. Furthermore, G. mellonella larvae infected with P. brasiliensis plus C. albicans presented a decrease in the survival rate compared to those infected with P. brasiliensis or C. albicans, mainly in the form of biofilms. Our data indicate that P. brasiliensis and C. albicans coexistence is likely to occur on oral mucosal biofilms, as per in vitro and in vivo analysis. These data further widen the knowledge associated with the dynamics of fungal biofilm growth that can potentially lead to the discovery of new therapeutic strategies for these infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Virulence factors are essential for establishing an invasive fungal infection, including dimorphism, host cell adhesion, cell wall composition, and hydrolytic enzyme production. (16,17,18,19,20,21,40) Another aspect of this disease is pulmonary fibrosis, which is char- acterised by excessive deposition of collagen and extracellular matrix components that results in pathological remodeling of the pulmonary architecture. (40) In this study, the expression of six genes in the Y and M forms of Pb18 and Pb01 after infection of two different cell types was determined using qPCR.…”

Section: Discussionmentioning

confidence: 99%

“…(14) Various components of Paracoccidioides spp., such as adhesins and others, may play a relevant role in pathogenesis. (16,17,1819,20,21) de Oliveira et al (22) evaluated the gene expression of different adhesins, including 14-3-3 protein, GP43, enolase (ENO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), triosephosphate isomerase (TPI), and malate synthase (MLS), after mice infection with P. brasiliensis and P. lutzii. (22) The study found differences among the two species, and that 14-3-3 and ENO showed the highest expression.…”

mentioning

confidence: 99%

Gene expression of Paracoccidioides virulence factors after interaction with macrophages and fibroblasts

Braz

Sardi

Pitangui

et al. 2021

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

BACKGROUND Paracoccidioidomycosis (PCM) is a systemic mycosis with high prevalence in Latin America that is caused by thermodimorphic fungal species of the Paracoccidioides genus.OBJECTIVES In this study, we used quantitative polymerase chain reaction (qPCR) to investigate the expression of genes related to the virulence of Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb01) strains in their mycelial (M) and yeast (Y) forms after contact with alveolar macrophages (AMJ2-C11 cell line) and fibroblasts (MRC-5 cell line).METHODS The selected genes were those coding for 43 kDa glycoprotein (gp43), enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 14-3-3 protein (30 kDa), phospholipase, and aspartyl protease. FINDINGS In the Pb18 M form, the aspartyl protease gene showed the highest expression among all genes tested, both before and after infection of host cells. In the Pb18 Y form after macrophage infection, the 14-3-3 gene showed the highest expression among all genes tested, followed by the phospholipase and gp43 genes, and their expression was 50-fold, 10-fold, and 6-fold higher, respectively, than that in the M form. After fibroblast infection with the Pb18 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 25-fold, 10-fold, and 10-fold higher, respectively, than that in the M form. Enolase and aspartyl protease genes were expressed upon infection of both cell lines. After macrophage infection with the Pb01 Y form, the 14-3-3 gene showed the highest expression, followed by the phospholipase and aspartyl protease genes, and their expression was 18-fold, 12.5-fold, and 6-fold higher, respectively, than that in the M form. MAIN CONCLUSIONSIn conclusion, the data show that the expression of the genes analysed may be upregulated upon fungushost interaction. Therefore, these genes may be involved in the pathogenesis of paracoccidioidomycosis.

show abstract

“…Cattana and collaborators (2017), demonstrated the presence of a biofilm ofP. brasiliensis in vivo in a 63-year-old patient with aortobifemural vascular prosthesis (Cattana et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Our research group demonstrated that P. brasiliensis is capable of forming biofilms in vitro ; one of the factors that influence biofilm formation is the presence of hydrolytic enzymes and specific adhesins in the yeast biofilm ( de Cássia Orlandi Sardi et al., 2015 ). Cattana and collaborators (2017), demonstrated the presence of a biofilm of P. brasiliensis in vivo in a 63-year-old patient with aorto-bifemural vascular prosthesis ( Cattana et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro

Medina-Alarcón

Silva

Ferin

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities.

show abstract

In Vivo Paracoccidioides sp. Biofilm on Vascular Prosthesis

Cited by 8 publications

References 7 publications

Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

Gene expression of Paracoccidioides virulence factors after interaction with macrophages and fibroblasts

Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro

Contact Info

Product

Resources

About