In-vivo performance of high-density collagen gel tubes for urethral regeneration in a rabbit model

Micol, Lionel A.; Silva, Luis F. Arenas da; Geutjes, Paul J.; Oosterwijk, Egbert; Hubbell, Jeffrey A.; Feitz, W.F.J.; Frey, Peter

doi:10.1016/j.biomaterials.2012.06.087

Cited by 52 publications

(42 citation statements)

References 12 publications

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“…To date, many attempts have been made to determine appropriate substitutes for urethral reconstruction, and in general, it is believed that seeding cells is necessary for constructing an artificial biomaterial scaffold that is suitable for reconstruction of extensive urethral defects. Various types of cells have been adopted in animal and clinical experiments, such as bone marrow mesenchymal stem cells [19][20][21], urethral epithelial cells [22] and smooth muscle cells [6,7,[23][24][25], urine-derived stem cells [26], mesothelial cells [27], adipose-derived stem Cells [28,29], and oral keratinocytes [30], and other cell types.…”

Section: Discussionmentioning

confidence: 99%

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Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Jia

Tang

et al. 2015

Biomaterials

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Section: Discussionmentioning

confidence: 99%

“…What's more, urine leakage is adverse to wound healing, and may be one of the reasons of urethral fistula and stricture. Truly tubular collagen scaffolds described in other studies [24,44] and fibrin glue may be helpful to reduce leakage and improve the outcome of urethral reconstruction.…”

Section: Accepted Manuscriptmentioning

confidence: 96%

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Jia

Tang

et al. 2015

Biomaterials

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“…These structures are mainly characterized by a transport function that can be severely impaired by several disorders such hypospadias, epispadias, and strictures, specifically referring to urethra [166]. In this regard, the implanted scaffold should promptly concur to recover the natural function offering a suitable environment that does not elicit any adverse reaction, particularly due to the toxic nature of the fluids in contact with the scaffold surface.…”

Section: Urinary Tractsmentioning

confidence: 99%

“…An example of urethral reconstruction by using a collagen tubular scaffold was proposed by Micol et al [166]. According to this approach a high density collagen gel tube was fabricated including autologous SMCs from bladder biopsy to repair a 1 cm defect in the rabbit urethra.…”

Section: Urinary Tractsmentioning

confidence: 99%

Tissue Engineered Scaffolds for an Effective Healing and Regeneration: Reviewing Orthotopic Studies

Baiguera

Urbani

Gaudio

2014

BioMed Research International

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It is commonly stated that tissue engineering is the most promising approach to treat or replace failing tissues/organs. For this aim, a specific strategy should be planned including proper selection of biomaterials, fabrication techniques, cell lines, and signaling cues. A great effort has been pursued to develop suitable scaffolds for the restoration of a variety of tissues and a huge number of protocols ranging from in vitro to in vivo studies, the latter further differentiating into several procedures depending on the type of implantation (i.e., subcutaneous or orthotopic) and the model adopted (i.e., animal or human), have been developed. All together, the published reports demonstrate that the proposed tissue engineering approaches spread toward multiple directions. The critical review of this scenario might suggest, at the same time, that a limited number of studies gave a real improvement to the field, especially referring to in vivo investigations. In this regard, the present paper aims to review the results of in vivo tissue engineering experimentations, focusing on the role of the scaffold and its specificity with respect to the tissue to be regenerated, in order to verify whether an extracellular matrix-like device, as usually stated, could promote an expected positive outcome.

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“…It is a ubiquitous molecule that can be extracted from animal tissues. Generally, collagen is well tolerated in vivo 27, 38, 46 . It presents cell adhesion sites and it can be digested enzymatically by cellular action.…”

Section: Introductionmentioning

confidence: 99%

Computer aided biomanufacturing of mechanically robust pure collagen meshes with controlled macroporosity

Islam¹,

Chapin²,

Younesi³

et al. 2015

Biofabrication

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Reconciliation of high strength and high porosity in pure collagen based structures is a major barrier in collagen’s use in load-bearing applications. The current study developed a CAD/CAM based electrocompaction method to manufacture highly porous patterned scaffolds using pure collagen. Utilization of computerized scaffold design and fabrication allows the integration of mesh-scaffolds with controlled pore size, shape and spacing. Mechanical properties of fabricated collagen meshes were investigated as a function of number of patterned layers, and with different pore geometries. The tensile stiffness, tensile strength and modulus ranges from 10-50 N/cm, 1-6 MPa and 5-40 MPa respectively for all the scaffold groups. These results are within the range of practical usability of different tissue engineering application such as tendon, hernia, stress urinary incontinence or thoracic wall reconstruction. Moreover, 3-fold increase in the layer number resulted in more than 5-fold increases in failure load, toughness and stiffness which suggests that by changing the number of layers and shape of the structure, mechanical properties can be modulated for the aforementioned tissue engineering application. These patterned scaffolds offer a porosity ranging from 0.8-1.5 mm in size, a range that is commensurate with pore sizes of repair meshes in the market. The connected macroporosity of the scaffolds facilitated cell-seeding such that cells populated the entire scaffold at the time of seeding. After 3 days of culture, cell nuclei became elongated. These results indicate that the patterned electrochemical deposition method in this study was able to develop mechanically robust, highly porous collagen scaffolds with controlled porosity which not only tries to solve one of the major tissue engineering problems in a fundamental level but also has a significant potential to be used in different tissue engineering applications.

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In-vivo performance of high-density collagen gel tubes for urethral regeneration in a rabbit model

Cited by 52 publications

References 12 publications

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Tissue Engineered Scaffolds for an Effective Healing and Regeneration: Reviewing Orthotopic Studies

Computer aided biomanufacturing of mechanically robust pure collagen meshes with controlled macroporosity

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