In vivo probiotic and antimicrobial photodynamic therapy as alternative therapies against cryptococcosis are ineffective

Oliveira, Lorena; Bastos, Rafael Wesley; Ribeiro, Noelly de Queiroz; Costa, Marliete Carvalho; Acúrcio, Leonardo Borges; Rocha, Karen Maia; Santos, Julliana Ribeiro Alves; Cruz, Rosana de Carvalho; Soares, Betânia Maria; Santos, Danielle Anjos dos

doi:10.1016/j.vetmic.2017.08.015

Cited by 5 publications

(1 citation statement)

References 37 publications

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“…Another study demonstrated in vitro that treatment with concentrated cell-free supernatant from the culture of L. plantarum 16 altered the transcription of genes involved in a variety of cellular functions, especially those related to cellular metabolism, which culminated in the complete inhibition of spore germination and development of the germ tubes and hyphae of the pathogen A. fumigatus ( Crowley et al, 2013 ). In one in vivo study using the probiotics Saccharomyces boulardii , L. paracasei ST-11, and L. rhamnosus GG, mice were not protected against lung infection caused by the pathogen Cryptococcus gattii ( Oliveira et al, 2017 ).…”

Section: Probiotics Prebiotics and Synbioticsmentioning

confidence: 99%

Gut Microbiota Modulation as a Potential Target for the Treatment of Lung Infections

2021

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The gastrointestinal and respiratory systems are colonized by a complex ecosystem of microorganisms called the microbiota. These microorganisms co-evolved over millions of years with the host, creating a symbiotic relationship that is fundamental for promoting host homeostasis by producing bioactive metabolites and antimicrobial molecules, and regulating the immune and inflammatory responses. Imbalance in the abundance, diversity, and function of the gut microbiota (known as dysbiosis) have been shown to increase host susceptibility to infections in the lungs, suggesting crosstalk between these organs. This crosstalk is now referred to as the gut-lung axis. Hence, the use of probiotics, prebiotics, and synbiotics for modulation of gut microbiota has been studied based on their effectiveness in reducing the duration and severity of respiratory tract infections, mainly owing to their effects on preventing pathogen colonization and modulating the immune system. This review discusses the role and responses of probiotics, prebiotics, and synbiotics in the gut-lung axis in the face of lung infections.

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Section: Probiotics Prebiotics and Synbioticsmentioning

confidence: 99%

Gut Microbiota Modulation as a Potential Target for the Treatment of Lung Infections

2021

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Assessment of the Safety of Lactobacillus casei IMV B-7280 Probiotic Strain on a Mouse Model

Лазаренко

Бабенко

Gichka

et al. 2021

Probiotics & Antimicro. Prot.

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Probiotics, in particular Lactobacillus (lactic acid bacteria, LAB) strains, are widely used in clinical practice. Despite that these probiotics have GRAS (generally regarded as safe) and qualified presumption of safety (QPS) statuses, the safety of particular strains still needs to be thoroughly studied. The aim of the study was to evaluate the safety of Lact. casei IMV B-7280 strain by investigating toxicity and the effects on gut microbiota in experimental animal model. Male BALB/c mice (7–8 weeks, weight 20–24 g) were treated with amounts of Lact. casei IMV B-7280 strain: 5 × 10 6 , 5 × 10 8 , or 5 × 10 9 CFU/animal once per day during 7 days, or in the amount of 1 × 10 10 CFU/animal once per day during 3 days (most of the proposed probiotic doses for humans—from 10 8 to 10 9 CFU) and monitored during 14 days. Blood tests and serum biochemistry were conducted; the cecal content from mice of the experimental and control groups were freshly collected and analyzed. At the end of the experiments (15th day), the presence of LAB in the heart, liver, kidney, and mesenteric lymph nodes and peripheral blood was determined; histology of the brain, liver, heart, fragments of the small and large intestine, and mesenteric lymph nodes was conducted. Survival rate of BALB/c mice treated with Lact. casei IMV B-7280 strain in different concentrations in toxicity experiments during 14 days was 100%. We observed no signs of toxicity as changes in gait, lethargy, sleep, somatomotor activity as well as changes in fur, eyes, skin and mucous membranes, tremors, behavior pattern, convulsions, salivation, diarrhea, and local injuries in mice from all experimental groups. After administration of probiotic strain, the number of opportunistic bacteria in cecal contents, such as Staphylococcus spp., Candida spp., Pseudomonas spp., and total aerobic and optionally anaerobic bacteria decreased compared to controls; the population of beneficial bacteria such as lactobacilli increased in cecal contents of these mice. LAB were not detected in the peripheral blood, heart, liver, kidneys, and mesenteric lymph nodes after administration of this strain to intact mice. Lact. casei IMV B-7280 strain is safe at dose up to 10 10 CFU/animal during 3- and 7-day oral administration to mice and has a positive effect on the gut microbiota composition; it could be potentially considered as safe probiotic for humans.

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Evidence of hypericin photoinactivation of E. faecalis: From planktonic culture to mammalian cells selectivity up to biofilm disruption

Amaral

Ribeiro

Perussi

2020

Photodiagnosis and Photodynamic Therapy

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In vivo probiotic and antimicrobial photodynamic therapy as alternative therapies against cryptococcosis are ineffective

Cited by 5 publications

References 37 publications

Gut Microbiota Modulation as a Potential Target for the Treatment of Lung Infections

Gut Microbiota Modulation as a Potential Target for the Treatment of Lung Infections

Assessment of the Safety of Lactobacillus casei IMV B-7280 Probiotic Strain on a Mouse Model

Evidence of hypericin photoinactivation of E. faecalis: From planktonic culture to mammalian cells selectivity up to biofilm disruption

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