In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel

Kang, Kkot Nim; Kim, Da Yeon; Yoon, So Mi; Kwon, Jin Seon; Seo, Hyo Won; Kim, E Sle; Lee, Bong; Kim, Jae Ho; Min, Byoung Hyun; Lee, Hai Bang; Kim, Moon Suk

doi:10.1016/j.ijpharm.2011.08.047

Cited by 23 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22,[41][42][43][44] The flexibility and mechanical properties of the NGC is an important factor for nerve regeneration to manipulate and resist stretching forces during implant surgery and to retain their shape during the nerve regeneration process. [24][25][26][27][28][29][30][31] Therefore, it is essential to explore the mechanical characteristics of nerve conduits. Our mechanical testing data and animal experiment results showed that the prepared SIS-and PCLA-NGCs had good flexibility and possessed sufficient toughness to resist compression or collapse upon implantation during peripheral nerve regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…SIS was obtained by complete removal of the tunica serosa and tunica muscularis using scraping with an edge of a Petri dish. [25][26][27][28][29][30]36 The obtained SIS was washed again with saline solution. The SIS was prepared by cutting in the longitudinal direction, followed by freeze drying using a freeze dryer (Model FDU-540; EYELA) at -80°C for 48 h to give native SIS sheets.…”

Section: Preparation Of Ngcsmentioning

confidence: 99%

“…Various SIS-based biomedical products have been developed and commercialized due to its good biocompatibility and nonimmunogenic property. [25][26][27][28][29][30][31] To our knowledge, however, SIS-based NGCs have received little attention at the early stages of in vivo research. 32 Thus, SIS may be considered as a candidate to easily give SIS-NGCs.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Small Intestine Submucosa and Poly(caprolactone-co-lactide) Conduits for Peripheral Nerve Regeneration

Shim

Kwon

Lee

et al. 2015

Tissue Engineering Part A

Self Cite

View full text Add to dashboard Cite

The present study employed nerve guidance conduits (NGCs) only, which were made of small intestine submucosa (SIS) and poly(caprolactone-co-lactide) (PCLA) to promote nerve regeneration in a peripheral nerve injury (PNI) model with nerve defects of 15 mm. The SIS-and PCLA-NGCs were easily prepared by rolling of a SIS sheet and a bioplotter using PCLA, respectively. The prepared SIS-and PCLA-NGCs fulfilled the general requirement for use as artificial peripheral NGCs such as easy fabrication, reproducibility for mass production, suturability, sterilizability, wettability, and proper mechanical properties to resist collapsing when applied to in vivo implantation. The SIS-and PCLA-NGCs appeared to be well integrated into the host sciatic nerve without causing dislocations and serious inflammation. All NGCs stably maintained their NGC shape for 8 weeks without collapsing, which matched well with the nerve regeneration rate. Staining of the NGCs in the longitudinal direction showed that the regenerated nerves grew successfully from the SIS-and PCLA-NGCs through the sciatic nerve-injured gap and connected from the proximal to distal direction along the NGC axis. SIS-NGCs exhibited a higher nerve regeneration rate than PCLANGCs. Collectively, our results indicate that SIS-and PCLA-NGCs induced nerve regeneration in a PNI model, a finding that has significant implications in the future with regard to the feasibility of clinical nerve regeneration with SIS-and PCLA-NGCs prepared through an easy fabrication method using promising biomaterials.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Ngcsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Small Intestine Submucosa and Poly(caprolactone-co-lactide) Conduits for Peripheral Nerve Regeneration

Shim

Kwon

Lee

et al. 2015

Tissue Engineering Part A

Self Cite

View full text Add to dashboard Cite

show abstract

“…The effective dose of growth factors in SIS or the proper amount of SIS gels is still controversial and needs to be confirmed. In current study, we used 20 wt% of SIS gels, because this concentration showed best gelation [18]. The optimal concentration and volume of SIS gel for parathyroid tissue implantation should be determined in further study.…”

Section: Discussionmentioning

confidence: 99%

Development of hypoparathyroidism animal model and the feasibility of small intestinal submucosa application on the parathyroid autotransplantation

Park¹,

Jung²,

Kim³

et al. 2014

Eur Arch Otorhinolaryngol

Self Cite

View full text Add to dashboard Cite

The purpose of this study is to evaluate the feasibility of small intestinal submucosa (SIS) application on the parathyroid autotransplantation in a rat model of hypoparathyroidism. The rats were divided into four groups: NC (no procedure, n = 5), PTX (total parathyroidectomy, n = 6), PT (total parathyroidectomy and parathyroid autotransplantation, n = 10) and PT + SIS group (total parathyroidectomy and parathyroid autotransplantation with SIS, n = 10). The levels of parathyroid hormone (PTH), calcium, and phosphorous were measured on 0, 3, 7, 21, 56 and 84 days after surgery. PTH level was expressed as median (interquartile range) and histological and immunohistochemical examinations were performed. PTH levels were significantly decreased to "not detectable level" from day 3 in PTX group. PTH was not detected in both PT and PT + SIS groups on the 21st day. On the 56th day, PTH levels were increased in both groups: 3 out of 8 rats (37.5%) in the PT group, 6 out of 9 rats (66.7%) in the PT + SIS group. The PTH level was fully recovered to its preoperative range on the day 84 as 6 of 8 rats (75%) of the PT group and 7 of 9 rats (77.8%) of the PT + SIS group were recovered; the PTH levels were 117.84 and 178.36 pg/ml, respectively. The neo-vascularization was well observed around the parathyroid tissue, and the number of new vessels formed was higher in the PT + SIS group (15 vessels/high power field) as compared to the PT group (10 vessels/high power field). This study showed the feasibility and the treatment effect of SIS as the success rate of autotransplantation of parathyroid tissue was significantly increased without severe inflammatory response in hypothyroidism animal model.

show abstract

“…Nonetheless, studies regarding the experimental utilization of SIS as drug carrier are limited . SIS is an excellent candidate as a drug carrier .…”

Section: Introductionmentioning

confidence: 99%

An Injectable, Click‐Cross‐Linked Small Intestinal Submucosa Drug Depot for the Treatment of Rheumatoid Arthritis

Kim

Park

et al. 2016

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Here, a click-cross-linked small intestine submucosa (SIS) drug depot is described for the treatment of rheumatoid arthritis (RA). To the best of the knowledge, there have been no studies related to the intra-articular injection of methotrexate (Met)-loaded click-cross-linkable SIS (Met-loaded Cx-SIS) for RA treatment. As the key objective of this work, injectable formulations of tetrazine-modified SIS (TE-SIS) and transcyclooctene-modified SIS (TC-SIS) are employed as drug depots. Within a few seconds, the simple mixing of equal amounts of TE-SIS and TC-SIS suspensions forms a gelatinous click-cross-linked SIS (Cx-SIS) drug depot in vitro and in vivo. The formed Cx-SIS depot is maintained in the articular joint over an extended period, while SIS alone rapidly disappears. Injectable formulations of Met-loaded Cx-SIS and Met-loaded SIS are prepared and then injected into articular joints to form drug depots. Compared to animals treated with Met-loaded SIS, RA animals treated with Met-loaded Cx-SIS show effective RA repair, as well as extensive regeneration of chondrocytes and glycosaminoglycan deposits. Collectively, these results indicate that the Met-loaded Cx-SIS depot is successfully formed after intra-articular injection of click-cross-linkable SIS, and that this formulation induces long-lasting Met release and allows Met to act effectively in the articular joint, resulting in RA repair.

show abstract

In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel

Cited by 23 publications

References 26 publications

Evaluation of Small Intestine Submucosa and Poly(caprolactone-co-lactide) Conduits for Peripheral Nerve Regeneration

Evaluation of Small Intestine Submucosa and Poly(caprolactone-co-lactide) Conduits for Peripheral Nerve Regeneration

Development of hypoparathyroidism animal model and the feasibility of small intestinal submucosa application on the parathyroid autotransplantation

An Injectable, Click‐Cross‐Linked Small Intestinal Submucosa Drug Depot for the Treatment of Rheumatoid Arthritis

Contact Info

Product

Resources

About