In vivo solid-phase microextraction swab-mass spectrometry for multidimensional analysis of human saliva

Wu, Lin; Yuan, Zi-Cheng; Yang, Bicheng; Huang, Zhengxu; Hu, Bin

doi:10.1016/j.aca.2021.338510

Cited by 26 publications

(13 citation statements)

References 42 publications

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“…Different MS approaches with various sampling, separation, and ionization techniques, such as gas chromatography (GC), liquid chromatography (LC), and inductively couple plasma (ICP), and matrix-assisted laser desorption/ionization (MALDI), can be used in omics research, biomarker discovers, qualitative and quantitative detection [ 36 ]. Particularly, ambient ionization (AI)-MS (e.g., paper spray [ 37 ]; desorption electrospray ionization, DESI) [ 38 ], and direct ionization (DI)-MS techniques (e.g., proton transfer reaction, PTR) [ 17 ], have been used for diagnosing COVID-19, and other direct ionization/sampling methods using direct sampling/ionization with medical swab [ 39 , 40 ] also show potential for COVID-19 studies. Significant MS-based metabolomic and proteomic studies on COVID-19-related human body fluids have been achieved [ 30 , 41 – 47 ].…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry-Based Human Breath Analysis: Towards COVID-19 Diagnosis and Research

Yuan

2021

J. Anal. Test.

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COVID-19 is a highly contagious respiratory disease that can be infected through human exhaled breath. Human breath analysis is an attractive strategy for rapid diagnosis of COVID-19 in a non-invasive way by monitoring breath biomarkers. Mass spectrometry (MS)-based approaches offer a promising analytical platform for human breath analysis due to their high speed, specificity, sensitivity, reproducibility, and broad coverage, as well as its versatile coupling methods with different chromatographic separation, and thus can lead to a better understanding of the clinical and biochemical processes of COVID-19. Herein, we try to review the developments and applications of MS-based approaches for multidimensional analysis of COVID-19 breath samples, including metabolites, proteins, microorganisms, and elements. New features of breath sampling and analysis are highlighted. Prospects and challenges on MS-based breath analysis related to COVID-19 diagnosis and study are discussed.

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Section: Introductionmentioning

confidence: 99%

Mass Spectrometry-Based Human Breath Analysis: Towards COVID-19 Diagnosis and Research

Yuan

2021

J. Anal. Test.

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“…The wooden tip was placed in the front of the mass spectrometer with distances of 0.8 cm horizontally from the wooden tip-end to the MS inlet via a nanoESI device (Thermo Fisher Scientific, Bremen, Germany). To load blood sample, whole blood samples (i.e., 1.0 µL) were directly loaded onto a wooden tip, and then organic solvent (5.0 µL of methanol/water/formic acid, 50/50/0.1, v/v/v), which is commonly used in spray ionization in ambient MS [45][46][47], was loaded onto the wooden tip in this work. With the application of a high voltage (3.0 kV) to wooden tip, spray ionization could be produced from wooden tip-end to acquire a mass spectrum.…”

Section: Wt-esi-ms Analysismentioning

confidence: 99%

Wooden-Tip Electrospray Mass Spectrometry Characterization of Human Hemoglobin in Whole Blood Sample for Thalassemia Screening: A Pilot Study

Huang

Zou

et al. 2022

Molecules

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Traditional analytical methods for thalassemia screening are needed to process complicated and time-consuming sample pretreatment. In recent decades, ambient mass spectrometry (MS) approaches have been proven to be an effective analytical strategy for direct sample analysis. In this work, we applied ambient MS with wooden-tip electrospray ionization (WT-ESI) for the direct analysis of raw human blood samples that were pre-identified by gene detection. A total of 319 whole blood samples were investigated in this work, including 100 α-thalassemia carriers, 67 β-thalassemia carriers, and 152 control healthy samples. Only one microliter of raw blood sample was directly loaded onto the surface of the wooden tip, and then five microliters of organic solvent and a high voltage of +3.0 kV were applied onto the wooden tip to generate spray ionization. Multiply charged ions of human hemoglobin (Hb) were directly observed by WT-ESI-MS from raw blood samples. The signal ratios of Hb chains were used to characterize two main types of thalassemia (α and β types) and healthy control blood samples. Our results suggested that the ratios of charged ions to Hb chains being at +13 would be an indicator for β-thalassemia screening.

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“…Štoviše, nakon uzorkovanja vlakno je moguće postaviti u nanoelektrosprej ionozacijsku kapilaru za brzu identifikaciju lijekova i njihovih metabolita masenom spekrometrijom. 62 Manousi i sur. 63 postavili su sol-gel sorbens u kapsulu te su ga primijenili za ekstrakciju ibuprofena iz urina.…”

Section: Nova Inovativna Rješenjaunclassified

Mikroekstrakcija čvrstom fazom – inovativni pristup u bioanalitičkim istraživanjima

Mornar

Marinac-Anđić

Klarić

et al. 2022

Kemija U Industriji

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In last three decades, the solid-phase microextraction technology has brought significant progress in bioanalytical research due to the versatility of this fast and solvent-free approach to biological sample preparation. It has been widely used in combination with various analytical instrumentation, even if most of the work has been done by coupling the extraction technique with liquid or gas chromatography. This review focuses on the new developments and advances in solid-phase microextraction-based techniques. Furthermore, some interesting, new innovations that fail to go beyond academic research are also reported.

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In vivo solid-phase microextraction swab-mass spectrometry for multidimensional analysis of human saliva

Cited by 26 publications

References 42 publications

Mass Spectrometry-Based Human Breath Analysis: Towards COVID-19 Diagnosis and Research

Mass Spectrometry-Based Human Breath Analysis: Towards COVID-19 Diagnosis and Research

Wooden-Tip Electrospray Mass Spectrometry Characterization of Human Hemoglobin in Whole Blood Sample for Thalassemia Screening: A Pilot Study

Mikroekstrakcija čvrstom fazom – inovativni pristup u bioanalitičkim istraživanjima

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