In vivo spectroscopic photoacoustic imaging and laser‐induced nanoparticle vaporization for anti‐HER2 breast cancer

Abstract

This study reports on the development and application of theragnostic agents targeting the HER2 receptors in breast tumors. The agent was constructed by loading silica-coated gold nanorods (GNRs) and a perfluorohexane liquid into PLGA-PEG nanoparticles, followed by surface conjugation with antibody Herceptin. The particle uptake in human breast cancer MDA-MB-231 (HER2negative) and BT474 (HER2-positive) cell lines was tested. A proof of principle in vivo study was also performed using a xenograft mouse bilateral tumor model (16 mice, 32 tumors). Photoacoustic imaging was performed using a VevoLAZR device at 720/750/850 nm illuminations and 21 MHz central frequency. The relative concentrations of GNRs in the tumor were quantified using a linear spectral unmixing technique. The therapeutic efficacy of these nanoparticles was evaluated through optical droplet vaporization, and cell damage was confirmed using tissue immunofluorescence and histology. Our results demonstrate the potential of PLGA-GNRs as theragnostic agents for anti-HER2 breast cancer therapy.

Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles

“…Wang et al developed herceptin-conjugated Poly (lactide-co-glycolic acid)-Polyethylene glycol (PLGA-PEG) NPs loaded with SiO 2 -coated gold nanorods and perfluorohexane liquid as therapeutic agent for laser-induced vaporization treatment and therapy of HER2-positive breast cancer. 122 In vitro and in vivo experiments using PAM demonstrated the efficient targeting of the particles to HER2-positive cells and mouse tumors. Also aiming at breast cancer therapy, Fu et al prepared doxorubicin (DOX)-loaded mesoporous Prussian blue NPs doped with the nitric oxide-producing prodrug sodium nitroprusside (NO-PB).…”

Enhancing Targeted Therapy in Breast Cancer by Ultrasound-Responsive Nanocarriers

“…Wang et al [ 202 ] further expanded upon the utility of ODV in phase-transition vectors in their experiment by utilizing silica-coated gold nanorods (GNR) and perfluorohexane (C6F14) into PLGA-PEG nanoparticles, which were further functionalized by surface conjugation of Herceptin antibodies. These particles were evaluated for site-specific accumulation and therapeutic efficacy against MDA-MB-231 (HER2- negative) and BT474 (HER2-positive) xenograft mouse models.…”

Advances of gold nanoclusters for bioimaging

“…AuNPs exhibiting surface plasmon resonance effects and NIR absorption have attracted much attention as exogenous contrast agents ( Li and Chen, 2015 ). AuNPs commonly used for PA imaging include Au nanorods, Au nanoflowers, Au nanocages, vesicles, and oversized spherical AuNPs (>5 nm in diameter) ( Chen et al., 2021 ; Huang et al., 2013 ; Santos et al., 2021 ; Wang et al., 2021 ; Yan et al., 2021 ).…”