In vivo sulfation of cholesterol by ascorbic acid 2-sulfate

Verlangieri, Anthony J.; Mumma, Ralph O.

doi:10.1016/0021-9150(73)90133-0

Cited by 32 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results indicate that AAS possesses inhibitory effects on the increase of lipid levels in serum and various organs, and a preventive effect on development of ather osclerosis. These results also suggest that the development of atherosclerosis was well As some authors suggested that AAS and AA are interconvertible in animal tissues (11,14), hypolipidemic and antiatherosclerotic effects of AAS may be elicited through the intermediary of AA. On the other hand, Verlangieri & Mumma (14) reported an increased excretion of cholesterol sulfate into feces in rats that had been given AAS by intracardial injection.…”

Section: Discussionsupporting

confidence: 61%

Fundamental Studies on Physiological and Pharmacological Actions of L-Ascorbate 2-Sulfate. V. On the Hypolipidemic and Anti Atherosclerotic Effects of L-Ascorbate 2-Sulfate in Rabbits

Hayashi¹,

Yamada²,

Kunitomo³

et al. 1978

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

The area rate of atheromatous plaque in aorta was less in specimens from both groups of AAS than in those from control I. Pathological changes in intima and media of aorta were milder in specimens from both groups of AAS.Development of pathological changes in arteries of various organs were prevented with both doses of AAS.The hypocholesterolemic effects of L-ascorbate 2-sulfate (AAS) in mice and rats have been reported in a previous paper (1) in which we suggested that AAS is a prospective hypolipidernic drug having a pronounced effect on lipid metabolism, extremely low toxicity and fewer pharmacological effects on the functions of various organs (2-4).In the present study the effects of AAS on experimental hyperlipidemia and athero sclerosis in rabbits were examined with L-ascorbate (AA) and clofibrate (CPIB) as reference compounds, and it was found that AAS possessed hypolipidemic effects in rabbits as well as in rats, and marked preventive effects on experimental atherosclerosis. MATERIALS AND METHODS Materials:The compounds examined for hypolipidemic effects are sodium L-ascorbate 2-sulfate-3 H2O, sodium L-ascorbate and clofibrate.Animal treatment: White male rabbits weighing approx. 2.5 kg were divided into 5 groups of nine and each animal was fed daily 100 g of laboratory chow (Nippon Clea, CR-1) supplemented with 1 of cholesterol. In addition to these 5 groups, one group of three

show abstract

Section: Discussionsupporting

confidence: 61%

Fundamental Studies on Physiological and Pharmacological Actions of L-Ascorbate 2-Sulfate. V. On the Hypolipidemic and Anti Atherosclerotic Effects of L-Ascorbate 2-Sulfate in Rabbits

Hayashi¹,

Yamada²,

Kunitomo³

et al. 1978

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

show abstract

“…Chemicals: [4][5][6][7][8][9][10][11][12][13][14] CJcholesterol (55.6 mCi/mmple), dissolved in benzene, was purchased from The Radiochemical Centre, Amersham. Ascorbic acid 2-sulfate (dipotassium salt), sodium ascorbate and cholesterol sulfate (ammonium salt) were supplied by Hoffmann-La Roche, Atlas G-1290 by Atlas" Chemie (Essen, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The in vivo sulfation of cholesterol by ascorbic acid 2-sulfate in the rat was recently reported (3,4). A single intracardial injection of [ 35 S]ascorbic acid 2-sulfate elevated the faecal excretion of cholesterol sulfate approximately fifty-fold.…”

mentioning

confidence: 94%

“…A single intracardial injection of [ 35 S]ascorbic acid 2-sulfate elevated the faecal excretion of cholesterol sulfate approximately fifty-fold. The hypocholesterolemic effect of ascorbic acid, as reported in man (5) as well as in laboratory animals (6,7), was postulated to be due to the intermediate synthesis of ascorbic acid 2-sulfate with subsequent transfer of the activated sulfate moiety onto cholesterol to form cholesterol sulfate which is then flushed out of the body (4). Ascorbic acid 2-sulfate was identified äs a significant metabolite of ascorbic acid in animals and man (8)(9)(10) and it has been suggested that it acts as a long-term storage form in the ascorbic acid pool of the body (11).…”

mentioning

confidence: 96%

“…Ascorbic acid 2-sulfate was identified äs a significant metabolite of ascorbic acid in animals and man (8)(9)(10) and it has been suggested that it acts as a long-term storage form in the ascorbic acid pool of the body (11). This report deals with the effect of ascorbic acid 2-sulfate, given over a period of 5 days by daily intravenous injection, on the faecal excretion of radioactivity after a single intravenous administration of [4][5][6][7][8][9][10][11][12][13][14] C]cholesterol in rats. The aim of this study was to examine whether the administration of ascorbic acid 2-sulfate enhances the faecal or urinary excretion of radioactive material, especially of cholesterol sulfate, and whether the accumulation of exogenous cholesterol by various tissues is decreased.…”

mentioning

confidence: 99%

See 2 more Smart Citations