In vivo temporal sequence of rat striatal glutamate, aspartate and dopamine efflux during apomorphine, nomifensine, NMDA and PDC in situ administration

Bert, Lionel; Parrot, Sandrine; Robert, F.; Desvignes, Christophe; Denoroy, Luc; Suaud-Chagny, Marie-Françoise; Renaud, Bernard

doi:10.1016/s0028-3908(02)00170-3

Cited by 43 publications

(44 citation statements)

References 43 publications

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“…In vivo microdialysis and CE-LIF were also used to investigate the interactions between dopamine (DA), Glu, and Asp in anesthetised rat striatum. The obtained results indicate that in the striatum endogenous DA and Glu may act in opposition to regulate each other's efflux [63]. Then, the hypothesis was put forward that large variability in striatal Glu and Asp levels may be due to a circannual fluctuation and such a possibility should be taken into account in the design of microdialysis experiments [64].…”

Section: Nda (442 Nm Hecd Laser or 405 Nm Diode Laser)mentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electrophoresis

2003

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Amino acids are studied extensively using capillary electrophoresis. In a previous article, we reviewed applications reported in the period 1999-early 2001 (Prata, C., Bonnafous, P., Fraysse, N., Treilhou, M., Poinsot, V., Couderc, F., Electrophoresis 2001, 22, 4129-4138). In this article we follow on with this review for the period end of 2001-beginning of 2003. We will report the developments of detection methods, separations of enantiomers, the new medical applications, and amino acids in food and plants. This review shows that CE is more and more important for the amino acid analysis.

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Section: Nda (442 Nm Hecd Laser or 405 Nm Diode Laser)mentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electrophoresis

2003

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“…When techniques such as HPLC are used, the temporal resolution is often 10-30 min; 7 however, coupling microdialysis to nanoscale analytical techniques such as capillary electrophoresis (CE), microbore liquid chromatography (LC), and electrochemical sensors have shortened sampling times to seconds. 4,[8][9][10][11][12][13][14][15][16][17][18] When using high sensitivity analytical methods, other factors can begin to limit temporal resolution achievable with microdialysis sampling. One inherent limitation is broadening of sample zones due to Taylor dispersion as they are transferred from sampling probe to analytical system.…”

Section: Introductionmentioning

confidence: 99%

Improved Temporal Resolution for in Vivo Microdialysis by Using Segmented Flow

et al. 2008

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Microdialysis sampling probes were interfaced to a segmented flow system to improve temporal resolution for monitoring concentration dynamics. Aqueous dialysate was segmented into nanoliter plugs by pumping sample stream into the base of a tee channel structure microfabricated on a PDMS chip that had an immiscible carrier phase (perfluorodecalin) pumped into the cross arm of the tee. Varying the oil flow rate from 0.22 to 6.3 μL/min and sample flow rate from 42 to 328 nL/min allowed control of plug volume, interval between plugs, and frequency of plug generation between 6 to 28 nL, 0.6 to 10 s, and 0.1 to 1.7 Hz, respectively. Temporal resolution of the system, determined by measuring fluorescence in individual sample plugs following step changes of fluorescein concentration at the sampling probe surface, was as good as 15 s. Temporal resolution was independent of both sampling flow rate and distance that samples were pumped from the sampling probe. This effect is due to the prevention of Taylor dispersion of the sample as it was transported by segmented flow. In contrast, without flow segmentation temporal resolution was worsened from 25 to 160 s as the detection point was moved from the sampling probe to 40 cm downstream. Glucose was detected by modifying the chip to allow enzyme assay reagents to be mixed with dialysate as sample plugs formed. The resulting assay had a detection limit of 50 μM and a linear range of 0.2 to 2 mM. This system was used to measure glucose in the brain of anesthetized rats. Basal concentration was 1.5 ± 0.1 mM (n = 3) and was decreased 60% by infusion of high K + solution through the probe. These results demonstrate the potential of microdialysis with segmented flow to be used for in vivo monitoring experiments with high temporal resolution.

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“…With such an approach, sampling times as short as 5-30 s (i.e., corresponding to submicroliter samples) can be performed (5 s [11], 10 s [12], 30 s [13]) allowing the monitoring of rapid changes associated to neuronal events. In this respect, CE-LIFD appears of a particular interest for the analysis of amino acid neurotransmitters and catecholamines [12].…”

Section: Introductionmentioning

confidence: 99%

In vivo simultaneous monitoring of γ‐aminobutyric acid, glutamate, and L‐aspartate using brain microdialysis and capillary electrophoresis with laser‐induced fluorescence detection: Analytical developments and in vitro/in vivo validations

et al. 2003

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gamma-Aminobutyric acid (GABA), glutamate (Glu), and L-aspartate (L-Asp) are three major amino acid neurotransmitters in the central nervous system. In this work, a method for the separation of these three neurotransmitters in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. Molecules were tagged on their primary amine function with the fluorogene agent naphthalene-2,3-dicarboxaldehyde (NDA), and, after separation by micellar electrokinetic chromatography, were detected by laser-induced fluorescence using a 442 nm helium-cadmium laser. The separation conditions for the analysis of derivatized neurotransmitters in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical basis. The separation of GABA, Glu, and L-Asp takes less than 10 min by using a 75 mmol/L borate buffer, pH 9.2, containing 70 mmol/L SDS and 10 mmol/L hydroxypropyl-beta-cyclodextrin and + 25 kV voltage. The detection limits were 3, 15 nmol/L and, 5 nmol/L for GABA, Glu, and L-Asp, respectively. Moreover, submicroliter samples can be analyzed. This method allows a simple, rapid and accurate measurement of the three amino acid neurotransmitters for the in vivo brain monitoring using microdialysis sampling.

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In vivo temporal sequence of rat striatal glutamate, aspartate and dopamine efflux during apomorphine, nomifensine, NMDA and PDC in situ administration

Cited by 43 publications

References 43 publications

Recent advances in amino acid analysis by capillary electrophoresis

Recent advances in amino acid analysis by capillary electrophoresis

Improved Temporal Resolution for in Vivo Microdialysis by Using Segmented Flow

In vivo simultaneous monitoring of γ‐aminobutyric acid, glutamate, and L‐aspartate using brain microdialysis and capillary electrophoresis with laser‐induced fluorescence detection: Analytical developments and in vitro/in vivo validations

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