In vivo time-course biocompatibility assessment of biomagnetic nanoparticles-based biomaterials for tissue engineering applications

Campos, Fernando; Bonhome-Espinosa, Ana B.; Carmona, Rafael Marfil; Durán, J.D.G.; Kuzhir, Pavel; Alaminos, Miguel; López–López, Modesto T.; Rodríguez, Ismael Ángel; Carriel, Víctor

doi:10.1016/j.msec.2020.111476

Cited by 31 publications

(20 citation statements)

References 57 publications

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“…These findings confirm the presence of cells within the hydrogels after different cell culture periods and suggest that they were able to proliferate within the hydrogels. In this connection, previous work by our group demonstrated that cells remained viable and were able to proliferate within hydrogels containing MNP, as determined by the quantification of DNA and WST-1 activity. , Future studies with longer culture periods are warranted to corroborate these preliminary results.…”

Section: Resultsmentioning

confidence: 59%

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Injectable Magnetic-Responsive Short-Peptide Supramolecular Hydrogels: Ex Vivo and In Vivo Evaluation

Mañas‐Torres

Gila-Vilchez

Vazquez-Perez

et al. 2021

ACS Appl. Mater. Interfaces

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The inclusion of magnetic nanoparticles (MNP) in a hydrogel matrix to produce magnetic hydrogels has broadened the scope of these materials in biomedical research. Embedded MNP offer the possibility to modulate the physical properties of the hydrogel remotely and on demand by applying an external magnetic field. Moreover, they enable permanent changes in the mechanical properties of the hydrogel, as well as alterations in the micro- and macroporosity of its three-dimensional (3D) structure, with the associated potential to induce anisotropy. In this work, the behavior of biocompatible and biodegradable hydrogels made with Fmoc-diphenylalanine (Fmoc-FF) (Fmoc = fluorenylmethoxycarbonyl) and Fmoc–arginine–glycine–aspartic acid (Fmoc-RGD) short peptides to which MNP were incorporated was studied in detail with physicochemical, mechanical, and biological methods. The resulting hybrid hydrogels showed enhance mechanical properties and withstood injection without phase disruption. In mice, the hydrogels showed faster and improved self-healing properties compared to their nonmagnetic counterparts. Thanks to these superior physical properties and stability during culture, they can be used as 3D scaffolds for cell growth. Additionally, magnetic short-peptide hydrogels showed good biocompatibility and the absence of toxicity, which together with their enhanced mechanical stability and excellent injectability make them ideal biomaterials for in vivo biomedical applications with minimally invasive surgery. This study presents a new approach to improving the physical and mechanical properties of supramolecular hydrogels by incorporating MNP, which confer structural reinforcement and stability, remote actuation by magnetic fields, and better injectability. Our approach is a potential catalyst for expanding the biomedical applications of supramolecular short-peptide hydrogels.

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Section: Resultsmentioning

confidence: 59%

“…In this connection, previous work by our group demonstrated that cells remained viable and were able to proliferate within hydrogels containing MNP, as determined by the quantification of DNA and WST-1 activity. 38 , 52 Future studies with longer culture periods are warranted to corroborate these preliminary results.…”

Section: Resultsmentioning

confidence: 61%

Injectable Magnetic-Responsive Short-Peptide Supramolecular Hydrogels: Ex Vivo and In Vivo Evaluation

Mañas‐Torres

Gila-Vilchez

Vazquez-Perez

et al. 2021

ACS Appl. Mater. Interfaces

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“…A comprehensive in vivo study regarding bioengineered magnetic fibrin-agarose tissue-like biomaterials was performed to optimize bionanostructures for tissue engineering and imaging applications. 150 The reported biomaterials are composed of a methyl methacrylate-co-hydroxyl ethyl methacrylate-co-ethylene glycol dimethacrylate coating polycrystalline magnetite (MMA-co-HEMA-co-EGDMA) core, were found to fully fulfill prerequested biosafety and biocompatibility aspects before its clinical use, allowing the efficient control of the MNPs biodistribution in vivo.…”

Section: Multifunctional Approachesmentioning

confidence: 99%

Magnetic Nanoparticles for Biomedical Applications: From the Soul of the Earth to the Deep History of Ourselves

Martins

Lima

Ribeiro

et al. 2021

ACS Appl. Bio Mater.

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Precisely engineered magnetic nanoparticles (MNPs) have been widely explored for applications including theragnostic platforms, drug delivery systems, biomaterial/device coatings, tissue engineering scaffolds, performance-enhanced therapeutic alternatives, and even in SARS-CoV-2 detection strips. Such popularity is due to their unique, challenging, and tailorable physicochemical/magnetic properties. Given the wide biomedical-related potential applications of MNPs, significant achievements have been reached and published (exponentially) in the last five years, both in synthesis and application tailoring. Within this review, and in addition to essential works in this field, we have focused on the latest representative reports regarding the biomedical use of MNPs including characteristics related to their oriented synthesis, tailored geometry, and designed multibiofunctionality. Further, actual trends, needs, and limitations of magneticbased nanostructures for biomedical applications will also be discussed.

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“…However, it will not allow to properly identify the lymphocytes (B, T (helper CD4+ and cytotoxic CD8+), natural killers (NKs)) or macrophages types (M1 or M2). The adequate interpretation of HE and immunohistochemical markers will allow to be determined an innate or acute immunological response mediated by neutrophils, macrophages, mast cells and NKs; an adaptive or chronic response from lymphocytes [T and B] and plasma cells; or foreign body reaction of mononuclear cells, macrophages and giant cells to TESSs [48,177,[203][204][205][206]. In addition, it is well-known that macrophages can acquire functionally distinct phenotypes, M1 and M2 macrophages.…”

Section: Histological and Ultrastructural Analysesmentioning

confidence: 99%

“…In addition, it is well-known that macrophages can acquire functionally distinct phenotypes, M1 and M2 macrophages. In general, M1 macrophages (express CD80, IFNγ, IL-6, IL-1β, MCP-1, iNOS, TNFα) exhibit strong proinflammatory properties whereas the M2 macrophages (express CD23, CD163, CD206, IL-10, IL-4R, TFGβ) appear to suppress immune surveillance promoting neovascularization [51,204,205].…”

Section: Histological and Ultrastructural Analysesmentioning

confidence: 99%

Basic Quality Controls Used in Skin Tissue Engineering

Linares‐González

Ródenas‐Herranz

Campos

et al. 2021

Life

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Reconstruction of skin defects is often a challenging effort due to the currently limited reconstructive options. In this sense, tissue engineering has emerged as a possible alternative to replace or repair diseased or damaged tissues from the patient’s own cells. A substantial number of tissue-engineered skin substitutes (TESSs) have been conceived and evaluated in vitro and in vivo showing promising results in the preclinical stage. However, only a few constructs have been used in the clinic. The lack of standardization in evaluation methods employed may in part be responsible for this discrepancy. This review covers the most well-known and up-to-date methods for evaluating the optimization of new TESSs and orientative guidelines for the evaluation of TESSs are proposed.

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In vivo time-course biocompatibility assessment of biomagnetic nanoparticles-based biomaterials for tissue engineering applications

Abstract: In vivo time-course biocompatibility assessment of biomagnetic nanoparticles-based biomaterials for tissue engineering applications

Cited by 31 publications

References 57 publications

Injectable Magnetic-Responsive Short-Peptide Supramolecular Hydrogels: Ex Vivo and In Vivo Evaluation

Injectable Magnetic-Responsive Short-Peptide Supramolecular Hydrogels: Ex Vivo and In Vivo Evaluation

Magnetic Nanoparticles for Biomedical Applications: From the Soul of the Earth to the Deep History of Ourselves

Basic Quality Controls Used in Skin Tissue Engineering

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