In vivo toxicological assessment of diquat dibromide: cytotoxic, genotoxic, and biochemical approach

Acar, Ali

doi:10.1007/s11356-021-13936-0

Cited by 12 publications

(6 citation statements)

References 63 publications

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“…The genotoxic influence of phenthoate observed in this research work might have been partly induced by the oxidative stress instigated by these substances. Various investigations have shown that these compounds alter redox status in plant cells, lending credence to this theory (Bonciu et al 2018;Acar 2021;Acar et al 2022). Phenthoate demonstrated a strong genotoxic effect on P. sativum plant in experimental conditions used in this investigation.…”

Section: Discussionsupporting

confidence: 71%

“…Genetic instability is caused by free radicles in cells. Reactive oxygen is extremely unstable, causing cytoskeleton disruption, imbalance in energy metabolism, and DNA harm, ensuing chromosomal anomalies (Acar et al 2021;Sengupta et al 2022). DNA damage is a primary biotic incidence which can harm biotic assemblies and procedures, as well as induce genotoxic disorders linked with the growth of carcinogenic developments (Kaur et al 2022;Ajermoun et al 2022;Zhang et al 2022).…”

Section: Discussionmentioning

confidence: 99%

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Phenthoate toxicity evaluation in root meristem of Pisum sativum L.

Siddiqui

2023

Caryologia

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Phenthoate is an organothiophosphate insecticide. Effect of phenthoate on the cytogenetic alterations in root tip cells of Pisum sativum L., a multiuse crop was investigated in this study. Pisum sativum L. seeds were exposed to different concentrations of phenthoate (0.1, 0.2, 0.3, 0.4, and 0.5%) and were germinated at 24°C for 72 hours and cytogenetic alterations were assessed. Analysis of mitotic index revealed that phenthoate has cytotoxic attributes, and cell proliferation kinetics frequencies showed alterations in the kinetics of the mitotic process. Phenthoate treatment of 0.1% to 0.5% resulted in an increase in the metaphases, and a reduction in prophases, anaphases, and telophases ratio, dose dependently. The findings of the study reveal that, phenthoate reduced the percentage of seed germination, mitotic index, radicle length and increased chromosomal abnormalities dose dependently. Root tip cells of Pisum sativum L. seeds treated with phenthoate showed an increased occurrence of single and double bridges, fragments, stickiness, laggard, and vagrants.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Phenthoate toxicity evaluation in root meristem of Pisum sativum L.

Siddiqui

2023

Caryologia

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“…The genotoxic effect of ME and IM found in this study could have partly been due to the oxidative stress caused by these agents. Several studies showed that these chemicals change the redox status of plant cells, which supports this theory [ 54 , 55 ]. Under the experimental settings used in this study, ME and IM had a potent genotoxic effect on the P. sativum plant.…”

Section: Discussionmentioning

confidence: 65%

“…Free radicals cause genomic instability in cells. Reactive oxygen species are very unstable and can disrupt the cytoskeleton, induce energy metabolism imbalance, and damage DNA, resulting in chromosomal abnormalities [ 37 , 54 ]. DNA damage is an initial biological phenomenon that can impair biological structures and processes, and cause genotoxic syndromes that are associated with the development of carcinogenic processes [ 21 , 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Exposure of Pisum sativum L. Seeds to Methomyl and Imidacloprid Cause Genotoxic Effects in Pollen-Mother Cells

Siddiqui

Alrumman

2022

Biology

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Pesticides are commonly used in modern agricultural systems to protect the plants from pests. Even though they potentially increase the crop yield, they have undesirable toxic effects on the consumers of plant products and nontarget host plants. However, there are limited studies to demonstrate the cytological changes induced by pesticides on plant cells. In the present study, we assess the cytological changes induced by two most commonly used insecticides, methomyl (ME) and imidacloprid (IM), using Pisum sativum L. as model plant system. P. sativum seeds were exposed to various concentrations of ME and IM (0.1, 0.2, 0.3, 0.4 and 0.5%) for 1, 3, and 6 h, and their effects on seed germination (SG), radicle length (RL), mitotic index (MI), chromosomal aberrations frequency (CAF), and micronucleus frequency (MNF) were studied. The results indicate that these insecticides decrease MI in root-tip cells, and increase in the MNF in pollen-mother cells in a dose-dependent manner. Additionally, insecticide-treated groups showed a dose- and time-dependent increase in the percentage of aberrant meiotic cells. Clumped nuclei (CNU), stickiness (STC), bridges (BRs), laggards (LGs), secondary association (SA), and precocious separation (PS) were among the frequently observed anomalies. The findings of this study indicate that commonly used insecticides ME and IM have substantial genotoxic effects on the root-tip and pollen-mother cells of P. sativum L.

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“…This herbicide acts on reduction−oxidation reactions, producing free radicals that interfere with the vital processes of plants, including protein synthesis. 52 The application of desiccant herbicides increased the activity of the catalase enzyme (Figure 3). The catalase activity (CAT) was higher in the treatment containing diquat + flumioxazin (T3), with an increase of 75.04% compared to the control (Figure 3).…”

Section: Resultsmentioning

confidence: 98%

Chemical Desiccation in the Preharvest of Cowpea: A Study of How the Time of Application Interferes in the Enzymatic and Physiological Aspects of Seedlings from Desiccated Plants

Coêlho,

Everthon da Silva Ribeiro,

Oliveira

et al. 2024

ACS Omega

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Chemical desiccation in the preharvest of grains and seeds is commonly used in production fields. Using herbicides for this purpose is a viable alternative to reduce beans' exposure to adverse crop conditions. Our objectives were to evaluate (1) the efficacy of herbicides for accelerated defoliation of cowpea, (2) the impact of herbicide application on antioxidant enzyme activity and protein and amino acid contents in seeds, and (3) the effects of different herbicide application schedules on the physiological aspects of seeds. In the first experiment, in addition to the control treatment (without herbicides), seven herbicides and two mixtures were applied at night: diquat, flumioxazin, diquat + flumioxazin, glufosinate ammonium, saflufenacil, carfentrazone, diquat + carfentrazone, atrazine, and glyphosate. Diquat and its mixtures showed greater efficacy in anticipating the harvest. Flumioxazin and diquat alone reduced amino acid content by 61.72 and 51.44%, respectively. The same trend was observed for total soluble proteins. The activity of antioxidant enzymes (CAT, POD, PPO) increased, indicating oxidative stress caused by diquat and flumioxazin. In the second experiment, we tested three application times (6 a.m., 12 p.m., 6 p.m.) with diquat, diquat + flumioxazin, and diquat + carfentrazone. The lowest damage to chlorophyll a was at 6 a.m.; other times reduced photosynthetic pigments and increased carotenoid content. Total soluble sugars decreased by 27.74% with nocturnal application of diquat + flumioxazin. Our data indicate that herbicide use for desiccation affects seed quality. These findings highlight the need for selecting appropriate herbicides and application times. Future research should explore long-term impacts on crop yield and quality.

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In vivo toxicological assessment of diquat dibromide: cytotoxic, genotoxic, and biochemical approach

Cited by 12 publications

References 63 publications

Phenthoate toxicity evaluation in root meristem of Pisum sativum L.

Phenthoate toxicity evaluation in root meristem of Pisum sativum L.

Exposure of Pisum sativum L. Seeds to Methomyl and Imidacloprid Cause Genotoxic Effects in Pollen-Mother Cells

Chemical Desiccation in the Preharvest of Cowpea: A Study of How the Time of Application Interferes in the Enzymatic and Physiological Aspects of Seedlings from Desiccated Plants

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