In vivo treatment with IL-17A attenuates hydatid cyst growth and liver fibrogenesis in an experimental model of echinococcosis

Labsi, Moussa; Soufli, Imene; Khelifi, Lila; Amir, Zine-Charaf; Touil-Boukoffa, Chafia

doi:10.1016/j.actatropica.2018.01.014

Cited by 21 publications

(16 citation statements)

References 34 publications

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“…KEGG pathway analysis further demonstrated that the mRNAs targeted by the miRNAs were mainly involved in the regulation of signal transduction, infectious diseases, and the immune system, which have been widely researched and demonstrated to be associated with the functions of exosomes (Coakley et al, 2015 ). The predicted host target genes are involved in the inflammatory responses, including encoding members of the TNF, TLR, MAPK, and NF-κB signaling pathways, which have previously been documented to modulate cytokines during chronic E. granulosus infection (Refik et al, 2005 ; Tuxun et al, 2015 ; Labsi et al, 2018 ; Zhang et al, 2019 ). Additionally, the most abundant miRNAs were predicted to participate in Th1 and Th2 cell differentiation, Th17 cell differentiation, and IL-17 signaling pathway, and these immune responses occur during E. granulosus infections (Tuxun et al, 2012 ; Labsi et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…The predicted host target genes are involved in the inflammatory responses, including encoding members of the TNF, TLR, MAPK, and NF-κB signaling pathways, which have previously been documented to modulate cytokines during chronic E. granulosus infection (Refik et al, 2005 ; Tuxun et al, 2015 ; Labsi et al, 2018 ; Zhang et al, 2019 ). Additionally, the most abundant miRNAs were predicted to participate in Th1 and Th2 cell differentiation, Th17 cell differentiation, and IL-17 signaling pathway, and these immune responses occur during E. granulosus infections (Tuxun et al, 2012 ; Labsi et al, 2018 ). These results indicated that these 20 most abundant miRNAs likely participate in parasite–host interactions and might regulate the host immune response.…”

Section: Discussionmentioning

confidence: 99%

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Comprehensive Analysis of Non-coding RNA Profiles of Exosome-Like Vesicles From the Protoscoleces and Hydatid Cyst Fluid of Echinococcus granulosus

Zhang

Gong

Cao

et al. 2020

Front. Cell. Infect. Microbiol.

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Cystic echinococcosis is a worldwide chronic zoonotic disease that threatens human health and animal husbandry. Exosome-like vesicles (ELVs) have emerged recently as mediators in the parasite-parasite intercommunication and parasite-host interactions. Exosome-like vesicles from parasites can transfer non-coding RNAs (ncRNAs) into host cells to regulate their gene expression; however, the ncRNAs profiles of the ELVs from Echinococcus granulosus remain unknown. Here, we isolated protoscolece (PSC)-ELVs and hydatid fluid (HF)-ELVs from the culture medium for E. granulosus PSCs in vitro and the HF of fertile sheep cysts, respectively. The microRNA (miRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA) profiles of the two types of ELVs were analyzed using high-throughput sequencing, and their functions were predicted using Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis. In PSC-ELVs and HF-ELVs, 118 and 58 miRNAs were identified, respectively, among which 53 miRNAs were present in both ELVs, whereas 65 and 5 miRNAs were unique to PSC-ELVs and HF-ELVs, respectively; 2,361 and 1,254 lncRNAs were identified in PSC-ELVs and HF-ELVs, respectively, among which 1,004 lncRNAs were present in both ELVs, whereas 1,357 and 250 lncRNAs were unique to PSC-ELVs and HF-ELVs, respectively. Intriguingly, the spilled PSCs from cysts excrete ELVs with higher numbers of and higher expression levels of miRNAs and circRNAs than HF-ELVs. The miRNA sequencing data were validated by quantitative reverse transcription-polymerase chain reaction. Furthermore, the target lncRNAs and mRNAs regulated by the 20 most abundant miRNAs were screened, and a ceRNA regulatory network containing 5 miRNAs, 41 lncRNAs, and 23 mRNAs was constructed, which provided new ideas and the molecular basis for further clarification of the function and mechanism of E. granulosus ELVs ncRNAs in the parasite-host interactions. Egr-miR-125-5p and egr-miR-10a-5p, sharing identical seed sites with host miRNAs, were predicted to mediate inflammatory response, collagen catabolic process, and mitogen-activated Zhang et al. NcRNAs in Echinococcus granulosus ELVs protein kinase cascade during parasite infections. In conclusion, for the first time, we identified the ncRNAs profiles in PSC-ELVs and HF-ELVs that might be involved in host immunity and pathogenesis, and enriched the ncRNAs data of E. granulosus. These results provided valuable resources for further analysis of the regulatory potential of ncRNAs, especially miRNAs, in both types of ELVs at the parasite-host interface.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Non-coding RNA Profiles of Exosome-Like Vesicles From the Protoscoleces and Hydatid Cyst Fluid of Echinococcus granulosus

Zhang

Gong

Cao

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Recent experimental studies show that Th1/Th17 polarization is a pivotal factor for resistance, while FoxP3 ϩ Tregs are key players in the immune regulatory processes favoring E. multilocularis metacestode survival (296). In vivo treatment of mice by a single intravenous injection of 200 l recombinant IL-17A at the optimal concentration of 125 pg/ml 2 weeks after E. granulosus sensu stricto infection decreased the infectivity rate by 2/3 and reduced metacestode growth by more than 90% (297). FoxP3 Treg depletion after infection using the DEREG (depletion of regulatory T cell) model in mice (296) and also genetic inhibition of the synthesis of fibrinogen-like protein 2 (FGL-2), a CD4 ϩ CD25 ϩ Treg effector molecule (298), are able to control E. multilocularis secondary infection.…”

Section: Improving Understanding Of Immunological Mechanisms Of Host-mentioning

confidence: 99%

Echinococcosis: Advances in the 21st Century

Wen¹,

Vuitton²,

et al. 2019

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SUMMARY Echinococcosis is a zoonosis caused by cestodes of the genus Echinococcus (family Taeniidae). This serious and near-cosmopolitan disease continues to be a significant public health issue, with western China being the area of highest endemicity for both the cystic (CE) and alveolar (AE) forms of echinococcosis. Considerable advances have been made in the 21st century on the genetics, genomics, and molecular epidemiology of the causative parasites, on diagnostic tools, and on treatment techniques and control strategies, including the development and deployment of vaccines. In terms of surgery, new procedures have superseded traditional techniques, and total cystectomy in CE, ex vivo resection with autotransplantation in AE, and percutaneous and perendoscopic procedures in both diseases have improved treatment efficacy and the quality of life of patients. In this review, we summarize recent progress on the biology, epidemiology, diagnosis, management, control, and prevention of CE and AE. Currently there is no alternative drug to albendazole to treat echinococcosis, and new compounds are required urgently. Recently acquired genomic and proteomic information can provide a platform for improving diagnosis and for finding new drug and vaccine targets, with direct impact in the future on the control of echinococcosis, which continues to be a global challenge.

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“…The restoration of the liver structure has been reported in mice infected by E. granulosus after treatment of 125 pg/mLIL-17A without any visible toxic effects. Lower fibrosis and reduced iNOS, TNF-α, NF-κb and CD68 expression in the hepatic parenchyma of treated mice were also noted [51].…”

Section: Therapeuticsmentioning

confidence: 99%

Mechanism of Fibrosis Induced by Echinococcus spp.

et al. 2019

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Infection with Echinococcus spp. causes fibrosis in various vital organs, including the liver and lungs. Hepatic fibrosis is a pathological feature of Echinococcus infection that destroys normal liver tissue, leading to jaundice, cholecystitis, portal hypertension, etc. Severe Echinococcus multilocularis infections lead to liver failure and hepatic encephalopathy. The formation of peripheral fiberboards around the metacestode is a major reason as to why antiparasitic drugs fail to be effectively transported to the lesion site. Studies on the mechanism of hepatic fibrosis caused by Echinococcus are important for treatment in patients. Recent studies have focused on miRNA and TGF-β. More recent findings have focused on the generation of collagen fibers around the metacestode. In this review paper we focus on the mechanism by which the Echinococcus parasite induces fibrosis in liver and some other organs in intermediate hosts—animals as well as human beings.

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In vivo treatment with IL-17A attenuates hydatid cyst growth and liver fibrogenesis in an experimental model of echinococcosis

Cited by 21 publications

References 34 publications

Comprehensive Analysis of Non-coding RNA Profiles of Exosome-Like Vesicles From the Protoscoleces and Hydatid Cyst Fluid of Echinococcus granulosus

Comprehensive Analysis of Non-coding RNA Profiles of Exosome-Like Vesicles From the Protoscoleces and Hydatid Cyst Fluid of Echinococcus granulosus

Echinococcosis: Advances in the 21st Century

Mechanism of Fibrosis Induced by Echinococcus spp.

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