In vivo ultrasound-switchable fluorescence imaging

Yao, Tingfeng; Yu, Shaoming; Liu, Yang; Yuan, Baohong

doi:10.1038/s41598-019-46298-2

Cited by 20 publications

(29 citation statements)

References 32 publications

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“…The coregistration between the USF and CT images indicates that the USF image is spread more in an axial direction than in a lateral direction. This is partially due to the nonuniform focal size of the ultrasound beam . The accomplishments of USF imaging with the ICG‐liposome in both mouse blood and porcine muscle tissue support the next ex vivo and in vivo USF imaging via IV injection.…”

Section: Discussionmentioning

confidence: 90%

“…An in‐house built frequency‐based USF imaging system, as proposed in previous published work, was utilized to conduct USF imaging with the ICG‐liposome. First, a tube model was used to determine the effect of blood on the performance of the ICG‐liposome.…”

Section: Methodsmentioning

confidence: 99%

“…The anesthesia machine (Supera Anesthesia Innovations, USA) was employed during the USF scan and 1.8% isoflurane (Miller Veterinary Supply, USA) was applied at 0.8 L min −1 . The imaging reconstruction and coregistration were conducted according to a previously published method …”

Section: Methodsmentioning

confidence: 99%

“…Recently, the adoption of new synthesis protocols has significantly improved the stability of ICG‐PNIPAM . With the stable ICG‐PNIPAM available, researchers recently reported in vivo USF imaging in a mouse for the first time . In addition, other USF contrast agents based on Pluronic micelles were also studied, showing a splendid performance with 4,4‐difluoro‐4‐bora‐3a,4a‐diaza‐s‐indacene (aza‐BODIPY) dyes in a phantom model .…”

Section: Introductionmentioning

confidence: 99%

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A Biocompatible and Near‐Infrared Liposome for In Vivo Ultrasound‐Switchable Fluorescence Imaging

Liu

Yao

Cai

et al. 2020

Adv Healthcare Materials

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Fluorescence imaging is a remarkable tool for molecular targeting and multicolor imaging, but it suffers from low resolution in centimeter‐deep tissues. The recently developed ultrasound‐switchable fluorescence (USF) imaging has overcome this challenge and achieved in vivo imaging in a mouse with help from the indocyanine green (ICG) dye encapsulated poly(N‐isopropylacrylamide) (ICG‐PNIPAM) contrast agent. However, the ICG‐PNIPAM has shortcomings, such as concerns about cytotoxicity and blueshifted excitation and emission spectra. This study introduces a newly developed ICG‐encapsulated liposome to broaden the contrast agent selection for USF imaging and resolve the issues mentioned above. The emission peak of the ICG‐liposome is 836 nm with excellent biostability and USF imaging capability. Furthermore, the cell viability test verifies the low cytotoxicity feature. Eventually, both ex vivo and in vivo USF imaging are successfully achieved and 3D USF images are acquired. The ex vivo result confirms that the ICG‐liposome maintains the thermoresponsive characteristic at the right lobe of the liver and is able to conduct the USF imaging. The further in vivo USF imaging demonstrates that although the whole liver emitted fluorescence, only the right lobe of the liver contains the working ICG‐liposome.

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Section: Discussionmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Biocompatible and Near‐Infrared Liposome for In Vivo Ultrasound‐Switchable Fluorescence Imaging

Liu

Yao

Cai

et al. 2020

Adv Healthcare Materials

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“…[13][14][15][16] Another technique is aimed at confining fluorescence emission only in a small volume through the interference of a FU and only switching on the fluorophores in the focal volume, so called ultrasound-switchable fluorescence (USF). [17][18][19][20][21][22][23][24][25][26][27][28] USF adopts a unique fluorescent contrast agent whose fluorescence can be switched on when temperature rises above a threshold (T th ). [19][20][21]23] When a FU beam is delivered into tissue, it induces a temperature rise in its focus.…”

Section: Introductionmentioning

confidence: 99%

In vivo ultrasound-switchable fluorescence imaging using a camera-based system

Yu¹,

Yao²,

Liu³

et al. 2020

Biomed. Opt. Express

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Ultrasound-switchable fluorescence (USF) is a novel imaging technique that provides high spatial resolution fluorescence images in centimeter-deep biological tissue. Recently, we successfully demonstrated the feasibility of in vivo USF imaging using a frequency-domain photomultiplier tube-based system. In this work, for the first time we carried out in vivo USF imaging via a camera-based USF imaging system. The system acquires a USF signal on a two-dimensional (2D) plane, which facilitates the image acquisition because the USF scanning area can be planned based on the 2D image and provides high USF photon collection efficiency. We demonstrated in vivo USF imaging in the mouse's glioblastoma tumor with multiple targets via local injection. In addition, we designed the USF contrast agents with different particle sizes (70 nm and 330 nm) so that they could bio-distribute to various organs (spleen, liver, and kidney) via intravenous (IV) injections. The results showed that the contrast agents retained stable USF properties in tumors and some organs (spleen and liver). We successfully achieved in vivo USF imaging of the mouse's spleen and liver via IV injections. The USF imaging results were compared with the images acquired from a commercial X-ray micro computed tomography (micro-CT) system.

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Exploring the Potential of Nanogels: From Drug Carriers to Radiopharmaceutical Agents

Kubeil,

Suzuki,

Casulli

et al. 2023

Adv Healthcare Materials

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Nanogels open up access to a wide range of applications and offer among others hopeful approaches for use in the field of biomedicine. This review provides a brief overview of current developments of nanogels in general, particularly in the fields of drug delivery, therapeutic applications, tissue engineering and sensor systems. Specifically, cyclodextrin (CD)‐based nanogels are important because they have exceptional complexation properties and are highly biocompatible. Nanogels as a whole and CD‐based nanogels in particular can be customized in a wide range of sizes and equipped with a desired surface charge as well as containing additional molecules inside and outside, such as dyes, solubility‐mediating groups or even biological vector molecules for pharmaceutical targeting. Currently, biological investigations are mainly carried out in vitro, but more and more in vivo applications are gaining importance. Modern molecular imaging methods are increasingly being used for the latter. Due to an extremely high sensitivity and the possibility of obtaining quantitative data on pharmacokinetic and pharmacodynamic properties, nuclear methods such as single photon emission computed tomography (SPECT) and positron emission tomography (PET) using radiolabeled compounds are particularly suitable here. The use of radiolabeled nanogels for imaging, but also for therapy, is being discussed.This article is protected by copyright. All rights reserved

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In vivo ultrasound-switchable fluorescence imaging

Cited by 20 publications

References 32 publications

A Biocompatible and Near‐Infrared Liposome for In Vivo Ultrasound‐Switchable Fluorescence Imaging

A Biocompatible and Near‐Infrared Liposome for In Vivo Ultrasound‐Switchable Fluorescence Imaging

In vivo ultrasound-switchable fluorescence imaging using a camera-based system

Exploring the Potential of Nanogels: From Drug Carriers to Radiopharmaceutical Agents

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