In vivo visualization of hippocampal cells and dynamics of Ca2+ concentration during anoxia: feasibility of a fiber-optic plate microscope system for in vivo experiments

Hirano, Masahiko; Yamashita, Yutaka; Miyakawa, Atsuo

doi:10.1016/0006-8993(96)00487-8

Cited by 24 publications

(19 citation statements)

References 39 publications

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“…Image-guiding bundles maintain the relative arrangements of the individual fibers throughout the length of the bundle, allowing transmission of an intensity image in pixilated form. Such bundles are commercially available and are commonly used for both epifluorescence 59 and scanning confocal or two-photon imaging 19,23,25,30,31,39,40,42,[60][61][62] . However, unlike in lenses or GRIN fibers, only intensity information is transmitted.…”

Section: Fiber Bundlesmentioning

confidence: 99%

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Fiber-optic fluorescence imaging

et al. 2005

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Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue. A challenge in the creation of such devices is the design and integration of miniaturized optical and mechanical components. Until recently, fiber-based fluorescence imaging was mainly limited to epifluorescence and scanning confocal modalities. Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging. An upcoming generation of fluorescence imaging devices will be based on microfabricated device components.Fiber-optic fluorescence imaging has become increasingly versatile over the last decade as fiber-based devices have declined in size but gained in functionality. Three classes of applications in live animal and human subjects provide the primary motivations for innovation in fiber-optic imaging. First, basic research on biological and disease processes would benefit enormously from instrumentation that permits cellular imaging under conditions in which conventional light microscopy cannot be used. Many cell types reside within hollow tissue tracts or deep within solid organs that are inaccessible to optical imaging without devices that can reach such locations in a minimally invasive manner. Flexible fiber-optic devices also allow handheld imaging and imaging in freely moving animals 1 . Second, fiber devices might be implanted within live subjects for long-term imaging studies. This capability will not only benefit research on the cellular effects of aging, development or experience, but also will lead to new in vivo assays for testing of drugs and therapeutics. By allowing examination of cells concurrently with observation of disease symptoms and animal behavior, fiber-optic imaging will permit studies correlating cellular properties and disease outcome in individual subjects over time and could reduce the numbers of animals needed. A third set of applications concerns development of minimally invasive clinical diagnostics and surgical procedures 2 . Although much work remains, fiber-optic instrumentation has already broadened the applicability of in vivo fluorescence imaging.The seeds for development of fiber optic in vivo imaging were planted by early uses of fiberoptics and fluorescence for chemical sensing and spectroscopy, including classic work on detection of intracellular redox states [3][4][5] . Use of fiber optics for remote sensing and spectroscopy remains strong today 6 , and has expanded to include bioluminescence detection 7 . The recent proliferation of fluorescent probes has widened the set of potential uses © 2005 Nature Publishing Group Correspondence should be addressed to M.J.S (mschnitz@stanford.edu). COMPETING INTERESTS STATEMENTThe authors declare that they have no co...

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Section: Fiber Bundlesmentioning

confidence: 99%

“…The use of fiber bundles alleviates this stipulation and provides mechanical flexibility while imaging in deep tissue 59 (Fig. 4b).…”

Section: Epifluorescence Imaging Modalitiesmentioning

confidence: 99%

Fiber-optic fluorescence imaging

et al. 2005

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“…First used in anesthetized preparations (133, 134), this method was quickly extended to freely moving mammalian systems. One group measured cortical Ca 2+ waves in newborn mice using a single implanted multimode fiber for both excitation and detection (91).…”

Section: Optical Readout Of Neural Activitymentioning

confidence: 99%

Optical Neural Interfaces

Warden

Cardin

Deisseroth

2014

Annu. Rev. Biomed. Eng.

176

134

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Genetically encoded optical actuators and indicators have changed the landscape of neuroscience, enabling targetable control and readout of specific components of intact neural circuits in behaving animals. Here, we review the development of optical neural interfaces, focusing on hardware designed for optical control of neural activity, integrated optical control and electrical readout, and optical readout of population and single-cell neural activity in freely moving mammals.

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“…Fortunately, radically new types of optical fibers (with hollow air-filled cores) now permit distortion-free propagation of femtosecond pulses (Göbel et al, 2004a). A second type of fiber-optic microscope is based on so-called coherent optical fiber bundles, which contain many thousands of fiber cores and transmit entire images, albeit with low spatial resolution (Hirano et al, 1996;Knittel et al, 2001;Gö-bel et al, 2004b). Fiber bundles are now available in small diameters and can be attached to small cylindrical lenses suitable for endoscopic imaging.…”

Section: Microscope Miniaturizationmentioning

confidence: 99%