In vivo wound healing and in vitro antioxidant activities of Bletilla striata phenolic extracts

Song, Yi; Zeng, Rui; Hu, Lingli; Maffucci, Katherine G.; Ren, Xiaodong; Qu, Yan

doi:10.1016/j.biopha.2017.06.079

Cited by 76 publications

(34 citation statements)

References 27 publications

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“…In general, our findings are in agreement with previous observations that natural antioxidants promote the wound healing [12, 48, 49]. The wound healing is a dynamic interaction between epidermal and dermal cells and extracellular cells, which occurs in three successive phases: inflammation, proliferation, and maturation.…”

Section: Resultssupporting

confidence: 93%

Dittrichia viscosaL. Ethanolic Extract Based Ointment with Antiradical, Antioxidant, and Healing Wound Activities

Rhimi

Hlel

Salem

et al. 2019

BioMed Research International

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Dittrichia viscosa which belongs to the Asteraceae family is frequently used to treat hematomas and skin disorders in Mediterranean herbal medicine. This study aims to validate its antioxidant effects and its potential on healing wounds. The ethanolic extract of D. viscosa leaves was formulated as 2.5% and 5% (w/w) in ointment bases on the beeswax and sesame oil. During this study, the ethanolic D. viscosa extract, ointments containing 2.5% and 5% of D. viscosa extract, and the vehiculum were assessed for their total phenol content (TPC), caffeoylquinic acid content (CQC), and antioxidant activities using complementary methods (TAC, the DPPH, ABTS, FRAP, and the BCB). The effects on wound healing of obtained ointments were evaluated by excision of the wound in a mice model for 12 days. Subsequently, the excised wound areas were measured at the 3rd, 9th, and 12th days. The skin tissues were isolated for histological studies. The ointments containing D. viscosa extract (2.5%, 5%) possessed a considerable TPC, CQC, radical scavenging potential, and antioxidant activities compared to the vehiculum. Treated animals with ointments containing D. viscosa extract at 2.5% and 5% showed almost and totally healed wounds compared to the vehiculum and control groups, evidenced by good skin regeneration and reepithelialization. The present work showed the role of D. viscosa antioxidants exerted by its polyphenolic compounds, in particular, caffeoylquinic acids, in enhancing wound healing.

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Section: Resultssupporting

confidence: 93%

Dittrichia viscosaL. Ethanolic Extract Based Ointment with Antiradical, Antioxidant, and Healing Wound Activities

Rhimi

Hlel

Salem

et al. 2019

BioMed Research International

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“…In this study, we found that D. fortunei water extract containing the similar concentration of PA to exert the angiogenesis, migration, and wound healing process. The possible molecular mechanism affecting the angiogenic properties of PA is via a programmed PI3K/Akt/eNOS/VEGF signaling axis which promotes neovascularization and invasion ( Kang et al, 2013 ; Song Y. et al, 2017 ). Moreover, PA had a strong inhibitory effect on fibroblast over-proliferation and keloid formation ( Phan et al, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Drynaria fortunei Promoted Angiogenesis Associated With Modified MMP-2/TIMP-2 Balance and Activation of VEGF Ligand/Receptors Expression

et al. 2018

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Background and Purpose: Drynaria fortunei J. Sm (D. fortunei), known as Gu-Sui-Bu, is used in traditional Chinese medicine to treat common injuries, including bone fractures and bruising. The specific functional mechanisms of the angiogenic and endothelial cell migration properties of D. fortunei are currently unclear. Thus, the purpose of this study is to validate the potential angiogenic and cellular migration properties and related mechanisms by D. fortunei both in vivo and in vitro.Experimental Approach: The present study investigates, both in vivo and in vitro, the wound healing effects of D. fortunei as associated with angiogenesis, specifically by the modulation of matrix metalloproteinases (MMPs) and upregulation of vascular endothelial growth factor (VEGF) ligand/receptors. In order to determine the potential angiogenic effects of D. fortunei, in vivo neovascularization of chick chorioallantoic membranes (CAMs) assay, and directed in vivo angiogenesis assay (DIVVA) were performed, while in vitro scratch wound healing, migration, and matrix-induced tube formation assays were performed by using human umbilical vascular endothelial cells (HUVECs). Furthermore, we used qPCR to analyze the gene expressions and Western blot to observe protein expressions of MMP-2, MMP-14, TIMP-2, RECK, and VEGF/VEGFRs.Results: This study identified five major compounds from the water extract of D. fortunei: protocatechuic acid, caffeic acid 4-O-β-D-glucopyranoside, 5,7-dihydroxychromone-7-O-rutinoside, neoeriocitrin, and naringin. D. fortunei was confirmed to activate in vivo angiogenesis by CAM and DIVVA assays. D. fortunei further exhibited in vitro angiogenic effects associated with cell migration, as demonstrated by the tube formation assay, transwell migration assay, and scratch wound healing assay. The extracellular MMP-2 activity was found to be dose-dependently augmented both in vitro and in vivo by D. fortunei. The mRNA and protein expressions of MMP-2, and MMP-14 were increased; while the tissue inhibitor metalloproteinase-2 (TIMP-2), and reversion-inducing cysteine-rich protein with kazal motifs (RECK) were both decreased. Furthermore, D. fortunei activated the gene and protein expressions of VEGF-A, -B, and VEGFR-2, -3.Conclusion: D. fortunei increased MMP-2 activity, thereby stimulating angiogenesis and cell migration, both in vivo and in vitro, as a result of MMP-2 and TIMP-2 balance modulation and the activation of VEGF/VEGFRs expression.

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“…Bletilla striata. Bletilla striata, as a traditional Chinese herb, has been applied to treat alimentary canal mucosal damage, ulcers, bruises, and burns for thousands of years [43,44]. Previous studies have exhibited the antioxidant, anti-inflammatory, antitumour, antiviral, and antibacterial capabilities of Bletilla striata [45,46].…”

Section: Rhynchophyllinementioning

confidence: 99%

Application of Traditional Chinese Medicines in Postoperative Abdominal Adhesion

Liu

Feng

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Adhesion is a frequent complication after abdominal surgery. Although various methods have been applied to prevent and treat postoperative abdominal adhesion (PAA), few modern drugs designed for clinical applications have reached the expected preventive or therapeutic effect so far. There is an imperative to develop some new strategies for the treatment of PAA. Traditional Chinese medicine (TCM) has been widely practiced for thousands of years and played an indispensable role in the prevention and treatment of diseases. Modern medicine researchers have accepted the therapeutic effects of many active components derived from Chinese medicinal herbs. The review stresses the most commonly used TCM treatment, including Chinese medicinal herbals and monomers, TCM formulas, and acupuncture treatment.

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In vivo wound healing and in vitro antioxidant activities of Bletilla striata phenolic extracts

Cited by 76 publications

References 27 publications

Dittrichia viscosaL. Ethanolic Extract Based Ointment with Antiradical, Antioxidant, and Healing Wound Activities

Dittrichia viscosaL. Ethanolic Extract Based Ointment with Antiradical, Antioxidant, and Healing Wound Activities

Drynaria fortunei Promoted Angiogenesis Associated With Modified MMP-2/TIMP-2 Balance and Activation of VEGF Ligand/Receptors Expression

Application of Traditional Chinese Medicines in Postoperative Abdominal Adhesion

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