In vivo β-carotene skin permeation modulated by Nanostructured Lipid Carriers

Maretti, Eleonora; Leo, Eliana Grazia; Rustichelli, Cecilia; Truzzi, Eleonora; Siligardi, Cristina; Iannuccelli, Valentina

doi:10.1016/j.ijpharm.2021.120322

Cited by 19 publications

(5 citation statements)

References 87 publications

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“…The peak is slightly low because of a bit of excess surfactant. It is related to a previous study that found a low peak at 82 °C in a bit addition of emulsifiers (Maretti et al, 2021). The sharpest peak at 146.03 °C describes the breakdown of encapsulated beta carotene in a NLC.…”

Section: Ftir Spectroscopy Analysis Thermal Analysissupporting

confidence: 82%

Formulation and Characteristics of Red Palm Oil Nanostractured Lipid Carriers Prepared by Microemulsion Method and Its Application in Drinking Yoghurt

Elianarni

Raharjo

Supriyadi

2023

IFNP

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The aim of this study was to investigate the characteristic of red palm oil nanostructured lipid carriers (RPO-NLC) made with microemulsion method. The microemulsion procedures prepared with ratio Tween 80 : Span 20 (80:20 ; 70:30), lipid : surfactant (1:3.75 ; 1:4), red palm oil : palm stearin (8:2 ; 7:3 ; 6:4). The best particle size was found at ratio 80:20, 1:4, 6:4 respectively with 24.73 ± 0.06 nm, zeta potential -7.43, turbidity 0,183% and polydispersity index at 0.06. This formulation indicated a loading capacity of 1.97%, encapsulation efficiency of 99.57%, FTIR, DSC, and stable emulsion at room temperature storage for four weeks. This best formulation was applied in drinking yoghurt with ratio nanostructured lipid carrier and yoghurt 1:18. The pH of yoghurt drink containing RPO-NLC increased from 4.13 to 4.37, while its encapsulation stability decreased from 78.45% to 49.29%. The results of sensory evaluation showed no significant difference in color, aroma, but there was significant difference on the taste of drinking yoghurt.

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Section: Ftir Spectroscopy Analysis Thermal Analysissupporting

confidence: 82%

Formulation and Characteristics of Red Palm Oil Nanostractured Lipid Carriers Prepared by Microemulsion Method and Its Application in Drinking Yoghurt

Elianarni

Raharjo

Supriyadi

2023

IFNP

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“…Both the rapid stabilization of carotenoid accumulation and high release rates in the first hours of the carotenoids presented in PEC make theses carotenoids suitable for transdermal delivery, since the protective administration mechanisms for these bioactive compounds are required [56]. The PEC carotenoid results are consistent with those obtained for β-carotene and support those obtained on the HaCaT cell line.…”

Section: In Vitro Permeation Of Pec Carotenoidssupporting

confidence: 78%

Carotenoids from Persimmon (Diospyros kaki Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT

et al. 2021

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Persimmon (Diospyros kaki Thunb.) fruits are a remarkable source of carotenoids, which have shown protective effects against UV radiation in bacteria, fungi, algae, and plants. The aim of this study was to analyze the photoprotection provided by an acetone extract, rich in carotenoids and obtained from byproducts derived from the persimmon juice industry, against UV-induced cell death in the keratinocyte HaCaT cell line. For this purpose, the cytotoxicity and phototoxicity of carotenoid extract, as well as its intracellular reactive oxygen species (ROS) scavenging and anti-adhesive activities towards HaCaT cells, were evaluated. The in vitro permeation test provided information about the permeability of the carotenoid extract. Persimmon extracts, rich in carotenoids (PEC), were absorbed by HaCaT keratinocyte cells, which reduced the UV-induced intracellular ROS production in treated cells. Thus, PEC exerted a photoprotective and regenerative effect on UV-irradiated HaCaT cells, and this protection was UV dose-dependent. No cytotoxic effect was observed in HaCaT cultures at the concentration tested. PEC treatment also stimulated the adhesion capacity of skin microbiome to HaCaT cells, while exhibiting a significant anti-adhesive activity against all tested pathogens. In conclusion, PEC showed potential for use as a functional ingredient in skin-care products.

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“…Notably, the pattern of differences between RS and RRS in the initial tracking period suggests that Peak RS may reflect a longer-term dietary status than Peak RRS , which responds relatively quickly to reduction. Existing studies [46][47][48][49][50] have shown that carotenoids move to the skin surface and that the concentration of carotenoids is higher on the skin surface than on the deep skin layer, which can lead to different results depending on the measured skin depth and area. The delayed reaction of RS at the time of reduction can be seen due to the RS characteristics of sampling being closer to the skin surface than RRS.…”

Section: The Strengths Of This Studymentioning

confidence: 99%

Evaluation of a Commercial Device Based on Reflection Spectroscopy as an Alternative to Resonance Raman Spectroscopy in Measuring Skin Carotenoid Levels: Randomized Controlled Trial

Hwang,

Park,

Jung

et al. 2023

Sensors

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Resonance Raman spectroscopy (RRS) has been used as a reference method for measuring skin carotenoid levels (SCL), which indicate vegetable and fruit intake. However, RRS is not an easy-to-use method in SCL measurement due to its complicated implementation. In this study, a commercial spectrophotometer based on reflection spectroscopy (RS), which is relatively simple and inexpensive, was evaluated to confirm usability compared with RRS in measuring SCL. To investigate the agreement between RS and RRS, eighty participants were randomly assigned to a high-carotenoid diet group (21 mg/day of total carotenoids) or a control-carotenoid diet group (14 mg/day of total carotenoids) during a 6-week whole-diet intervention period and a 4-week tracking period. Strong correlations between the RS and RRS methods were observed at baseline (r = 0.944) and the entire period (r = 0.930). The rate of SCL increase was similar during the diet intervention; however, the initiation of the SCL decrease in RS was slower than in RRS during the tracking period. To confirm the agreement of RS and RRS from various perspectives, new visualization tools and indices were additionally applied and confirmed the similar response patterns of the two methods. The results indicate that the proposed RS method could be an alternative to RRS in SCL measurements.

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In vivo β-carotene skin permeation modulated by Nanostructured Lipid Carriers

Cited by 19 publications

References 87 publications

Formulation and Characteristics of Red Palm Oil Nanostractured Lipid Carriers Prepared by Microemulsion Method and Its Application in Drinking Yoghurt

Formulation and Characteristics of Red Palm Oil Nanostractured Lipid Carriers Prepared by Microemulsion Method and Its Application in Drinking Yoghurt

Carotenoids from Persimmon (Diospyros kaki Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT

Evaluation of a Commercial Device Based on Reflection Spectroscopy as an Alternative to Resonance Raman Spectroscopy in Measuring Skin Carotenoid Levels: Randomized Controlled Trial

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