Inability of highly purified preparations of human chorionic gonado‐tropin to inhibit the phytohemagglutinin‐induced stimulation of lymphocytes

Gundert, D.; Merz, Wolfgang E.; Hilgenfeldt, Ulrich; Brossmer, Reinhard

doi:10.1016/0014-5793(75)80043-3

Cited by 23 publications

(4 citation statements)

References 18 publications

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“…First of all, no correlation between the dose-dependent inhibition of lymphocyte transformation by different crude hormone batches and their gonadotropic activity could be found. The separation of the inhibitory activity in a fraction with a very small hormonal activity which is probably not significant, as well as the enrichment of the hormone in a second fraction of the same purification step, provide further support for our earlier findingsf 13 1. Choriogoftadotropin which had been purified in 3 steps inhibits lymphocyte transformation beyond a dose of 5000 IU/m/.…”

Section: Discussionsupporting

confidence: 70%

Separation of the Gonadotropic Activity of Crude Choriogonadotropin from the Inhibitory Effect on Lymphocyte Transformation

Merz¹,

Schmidt²,

Lenhard³

1979

Hoppe-Seyler´s Zeitschrift für physiologische Chemie

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The effect of Choriogonadotropin of different purities on the transformation of peripheral human lymphocytes was studied. Various crude hormone batches inhibited lymphocyte transformation in a dose-dependent manner, both in the mixed lymphocyte reaction and in the phytohemagglutinin-induced stimulation. The inhibitory activity, however, was found not to be correlated with the gonadotropic activity of the crude hormone batches (2660-4300 lU/mg). Choriogonadotropin (13000 lU/mg), which was purified in 3 steps, showed no inhibitory effect except at high doses (> 5000 IU/m/ final dilution). More detailed investigations provided evidence that in the first step of the Choriogonadotropin purification procedure (batch adsorption of crude Choriogonadotropin on SP-Sephadex C-50), the inhibitory activity can be enriched in a fraction (Fract. I) which displays a very low gonadotropic activity (< 500 lU/mg). A further separation of Fract. I was achieved by isoelectric focusing as well as by chromatography on DEAESephadex A-25. By these means, the inhibitory potency could be enriched more than 100-fold. The substances which display inhibition of DNA synthesis in lymphocytes were proven to act in a nontoxic way. A preliminary characterization of the strongly inhibiting substances which show a dose-dependent suppression of lymphocyte transformation by about 99%, showed that this effect is probably exerted via non-dialysable sialoglycoproteins.By a fourth purification step entailing a chromatography of purified Choriogonadotropin (13000 lU/mg) on SP-Sephadex C-50, a highly purified Choriogonadotropin (14000 lU/mg) could be obtained which showed no inhibitory effect on lymphocyte transformation (in both mixed lymphocyte reaction and in phytohemagglutinin-induced stimulation) up to a dose of 43000 IU/m/. The components which were removed from Choriogonadotropin in this step seem to be immunologically identical with the strongly inhibiting substances isolated by isoelectric focusing.These investigations demonstrate that biologically active, highly purified Choriogonadotropin is unable to inhibit lymphocyte transformation. The inhibitory activity of crude hormone can be enriched in choriogonadotropin-free fractions. Therefore, it is concluded that the inhibitory activity of crude hormone is not a property of Choriogonadotropin itself.

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Section: Discussionsupporting

confidence: 70%

Separation of the Gonadotropic Activity of Crude Choriogonadotropin from the Inhibitory Effect on Lymphocyte Transformation

Merz¹,

Schmidt²,

Lenhard³

1979

Hoppe-Seyler´s Zeitschrift für physiologische Chemie

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“…It is possible that hCG stimulates P4 production by tropho blasts. Some investigators have envisioned a role for hCG in immunologic protection of the trophoblast (173); others have suggested this activity is due to a contaminant in hCG preparations (72,102). Because of the biological and immunological similarity between hCG and LH, it was difficult previ ously to distinguish between these two gonadotropins.…”

Section: Protein Hormone Production By the Placentamentioning

confidence: 98%

Endocrine Physiology of the Placenta

Simpson¹,

MacDonald²

1981

Annu. Rev. Physiol.

225

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“…It has been reported that human pregnancy serum inhibits mitogen-stimulated lymphocyte proliferation (Walker et al, 1972;Jones et al, 1973;Figueredo et al, 1979). Crude human chorionic gonadotrophin (hCG) preparations had similar effect (Adcock et al, 1973), while highly purified hCG did not influence the lymphocyte response to mitogens, suggesting that the lymphocyte suppression observed was due to additional unidentified substances present in the crude hormone preparations (Caldwell et al, 1975;Gundert et al, 1975). Alpha-fetoprotein was found to effectively suppress T-cell mediated reactions but not B-cell responses in vitro (Murgita et al, 1978).…”

Section: Discussionmentioning

confidence: 99%

The Distribution of Polyamine Oxidase Activity in the Fetomaternal Compartments

Illei

Morgan

1979

BJOG

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Summary Polyamine oxidase activity was measured in different compartments of the feto‐maternal unit by a radiochemical method. The activity in the retroplacental serum (mainly of intervillous origin) was 20 to 30 times higher than in maternal uterine or peripheral venous blood sera. No activity was found in the fetal cord blood sera. The enzyme level in the maternal peripheral sera fell to undetectable levels within 72 hours post partum. Preliminary data indicate that the enzyme might be produced by the decidua rather than by the placenta. It is suggested that the enzyme may constitute an important part of a supposed local immunological barrier formed at the placental bed to help to protect the fetal allograft from maternal immune rejection through a local suppressive effect on maternal cellular immunity.

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Inability of highly purified preparations of human chorionic gonado‐tropin to inhibit the phytohemagglutinin‐induced stimulation of lymphocytes

Cited by 23 publications

References 18 publications

Separation of the Gonadotropic Activity of Crude Choriogonadotropin from the Inhibitory Effect on Lymphocyte Transformation

Separation of the Gonadotropic Activity of Crude Choriogonadotropin from the Inhibitory Effect on Lymphocyte Transformation

Endocrine Physiology of the Placenta

The Distribution of Polyamine Oxidase Activity in the Fetomaternal Compartments

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