Inactivación de micobacterias con desinfectantes registrados como tuberculicidas

Bello, Teresita; Rivera-Olivero, Ismar A.; Waard, Jacobus H. de

doi:10.1157/13089667

Cited by 18 publications

(12 citation statements)

References 11 publications

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“…Further, we have confirmed that M. abscessus and M. chelonae are significantly more resistant to QACs than M. smegmatis 29, 30. Although the activities of different QACs vary somewhat depending upon the mycobacterial species, DBLAB was the least effective of those tested.…”

Section: Discussionsupporting

confidence: 62%

Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

Cortesia

Bello

López

et al. 2017

Brazilian Journal of Microbiology

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Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recently been many non-tuberculous mycobacteria soft tissue infections after minor surgical procedures, some apparently related to the use of a commercial disinfectant based on a Quaternary Ammonium Compound. We studied the activity of this and other quaternary ammonium compounds on different non-tuberculous mycobacteria by transforming the mycobacteria with a dnaA-gfp fusion and then monitoring fluorescence to gauge the capacity of different quaternary ammonium compounds to inhibit bacterial growth. The minimum inhibitory concentration varied for the different quaternary ammonium compounds, but M. chelonae and M. abscessus were consistently more resistant than M. smegmatis, and M. terrae more resistant than M. bovis BCG.

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Section: Discussionsupporting

confidence: 62%

Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

Cortesia

Bello

López

et al. 2017

Brazilian Journal of Microbiology

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“…QACs are described in the literature as only mycobacteriostatic, so we were surprised to find that a 20 min exposure to QACs appeared to be cidal against M. smegmatis , with at least 5 logs of killing; 14 although their effectiveness would likely be less if assayed in a carrier test in the presence of organic material. 15 Against M. fortuitum , M. chelonae , M. massiliense and M. abscessus , however, even with the simple suspension test employed here, we found that QACs kill only ∼90%–95% of bacteria.…”

Section: Discussionmentioning

confidence: 89%

The use of quaternary ammonium disinfectants selects for persisters at high frequency from some species of non-tuberculous mycobacteria and may be associated with outbreaks of soft tissue infections

Cortesia

López

Waard

et al. 2010

Journal of Antimicrobial Chemotherapy

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BackgroundNon-tuberculous mycobacteria (NTM) are increasingly important as opportunistic infections after major and minor surgical procedures, likely because they are ubiquitous and not effectively killed by many commonly used disinfectants. Outbreaks of soft tissue infections with NTM appeared related to the use of commercial disinfectants based on quaternary ammonium compounds (QACs).MethodsWe studied the survival of clinical and environmental isolates of Mycobacterium abscessus, Mycobacterium massiliense, Mycobacterium chelonae and Mycobacterium fortuitum after 20 min, 60 min or 24 h exposures to different QACs, and the surviving bacteria were then re-exposed to QACs to see if the percentage of surviving bacteria had increased. The bacteria were labelled with a dnaA–gfp fusion and their level of QAC resistance monitored as increasing fluorescence. The QAC-resistant bacteria were then serially restreaked onto non-selective medium and retested for QAC survival.ResultsThe frequency of survivors was <1 in 105 bacteria with Mycobacterium smegmatis, but >1 in 100 with the other mycobacteria studied. Different environmental and clinical isolates had similar QAC MICs, but QAC survivors of each strain were resistant. The QAC-surviving strains reverted to the original, non-resistant phenotype after several passages on non-selective medium.ConclusionsQACs should not be used in settings where even minimally invasive procedures are performed, as they select for a non-genetically determined reversible resistant phenotype that appears at high frequency with several rapidly growing mycobacterial species associated with healthcare-related infections. M. smegmatis behaves differently and is not an adequate model for testing the activity of disinfectants against NTM.

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“…Cidex and Cidex OPA were effective against tested organisms, showing greater than a 5-log reduction in colony-forming units (CFU) after 5 min of exposure under both clean and dirty conditions [13]. …”

Section: Discussionmentioning

confidence: 99%

Evaluation of mycobactericidal activity of selected chemical disinfectants and antiseptics according to European standards

2014

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BackgroundThe history of the investigation of standardized mycobactericidal activity of disinfectants and antiseptics is not very long. There is growing interest among the manufacturers of disinfectants in carrying out research on the antimicrobial activities in accordance with European standards (EN). This research could facilitate the introduction of high-quality disinfectants to the market. The aim of this study was to evaluate the mycobactericidal activity of selected chemical disinfectants and antiseptics used in the medical and veterinary fields.Material/MethodsThis study included 19 products submitted to the National Medicines Institute in Poland for evaluation of mycobactericidal activity. These products contain in their composition active substances belonging to different chemical groups, including aldehydes, alcohols, amines, quaternary ammonium compounds, phenols, guanidine, and oxidizing compounds. This study, conducted according to the manufacturers’ description of the preparations, was carried out in accordance with European standards, which also met the Polish standards: PN-EN 14204: 2013, PN-EN 14348: 2006, and PN-EN 14563: 2012.ResultsTested products for disinfection and antiseptics containing active substances from different chemical groups showed high mycobactericidal activity and met the requirements of the appropriate European standards in most cases. In the case of products containing guanidine and amine compounds, the concentration of active ingredients used in the test and the test conditions specified by the manufacturer did not provide the mycobactericidal activity required by the standards.ConclusionsPrior to the launch of a new product on the market, it is important to establish the appropriate usage and testing conditions of the preparation, such as its practical concentration, contact time, and environment condition (clean or dirty).

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Inactivación de micobacterias con desinfectantes registrados como tuberculicidas

Cited by 18 publications

References 11 publications

Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

The use of quaternary ammonium disinfectants selects for persisters at high frequency from some species of non-tuberculous mycobacteria and may be associated with outbreaks of soft tissue infections

Evaluation of mycobactericidal activity of selected chemical disinfectants and antiseptics according to European standards

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