Inactivation and adaptation of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria when exposed to free nitrous acid

Ma, Bin; Liu, Yang; Wang, Qilin; Yuan, Zhiguo; Wang, Yayi; Peng, Yongzhen

doi:10.1016/j.biortech.2017.08.074

Cited by 105 publications

(57 citation statements)

References 14 publications

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“…Previous nitrite pathway studies with FNA treatment (Ma et al, 2017a;Wang et al, 2017;Wang et al, 2014) did not reach a nitrite accumulation ratio of 90%, likely due to the relatively low FNA concentration applied.…”

Section: Effects Of Fna Concentration On the Nitrite Pathwaymentioning

confidence: 86%

“…In comparison, with 50% inactivation rate to AOB after the FNA treatment, the SRTs for AOB (SRTAOB) remained around 5.7, 4.5 and 3.9 days for R2, R3 and R4, respectively. Another considerable difference between the batch tests and the long-term reactor tests is that some strains of AOB or NOB in the reactor may gradually acclimate to FNA treatment (Ma et al, 2017a). The adaptation of some strains of AOB and NOB might be resulted from two possibilities, acclimation to short SRT or resistance to FNA treatment, or combined.…”

Section: Effects Of Fna Treatment Frequency On the Nitrite Pathwaymentioning

confidence: 99%

“…Since the initial proof-of-concept study (Wang et al, 2014), two following up studies were conducted. In all these three studies, the FNA treatment conditions were chosen based on either batch test results or educated guess, resulting in various extents of success (Wang et al, 2017) and even complete failure (Ma et al, 2017a). Ma et al (2017a) failed to establish the nitrite pathway with FNA treatment due to the observed adaptation of NOB to FNA.…”

Section: Introductionmentioning

confidence: 99%

“…In all these three studies, the FNA treatment conditions were chosen based on either batch test results or educated guess, resulting in various extents of success (Wang et al, 2017) and even complete failure (Ma et al, 2017a). Ma et al (2017a) failed to establish the nitrite pathway with FNA treatment due to the observed adaptation of NOB to FNA. We hypothesise that the adaptation observed in the study was caused by the inappropriate FNA treatment conditions applied.…”

Section: Introductionmentioning

confidence: 99%

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Effects of free nitrous acid treatment conditions on the nitrite pathway performance in mainstream wastewater treatment

Duan

Wang

Erler

et al. 2018

Science of The Total Environment

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Inline sludge treatment using free nitrous acid (FNA) was recently shown to be effective in establishing the nitrite pathway in a biological nitrogen removal system. However, the effects of FNA treatment conditions on the nitrite pathway performance remained to be investigated. In this study, three different FNA treatment frequencies (daily sludge treatment ratios of 0.22, 0.31 and 0.38, respectively), two FNA concentrations (1.35 mgN/L and 4.23 mgN/L, respectively) and two influent feeding regimes (one- and two-step feeding) were investigated in four laboratory-scale sequencing batch reactors. The nitrite accumulation ratio was positively correlated to the FNA treatment frequency. However, when a high treatment frequency was used e.g., daily sludge treatment ratio of 0.38, a significant reduction in ammonia oxidizing bacteria (AOB) activity occurred, leading to poor ammonium oxidation. AOB were able to acclimatise to FNA concentrations up to of 4.23 mgN/L, whereas nitrite oxidizing bacteria (NOB) were limited by an FNA concentration of 1.35 mgN/L over the duration of the study (up to 120 days). This difference in sensitivity to FNA could be used to further enhance nitrite accumulation, with 90% accumulation achieved at an FNA concentration of 4.23 mgN/L and a daily sludge treatment ratio of 0.31 in this study. However, this high level of nitrite accumulation led to increased NO emission, with emission factors of up to 3.9% observed. The NO emission was mitigated (reduced to 1.3%) by applying two-step feeding resulting in a nitrite accumulation ratio of 45.1%. Economic analysis showed that choosing the optimal FNA treatment conditions depends on a combination of the wastewater characteristics, the nitrogen discharge standards, and the operational costs. This study provides important information for the optimisation and practical application of FNA-based sludge treatment technology for achieving the mainstream stable nitrite pathway.

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Section: Effects Of Fna Concentration On the Nitrite Pathwaymentioning

confidence: 86%

Section: Effects Of Fna Treatment Frequency On the Nitrite Pathwaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of free nitrous acid treatment conditions on the nitrite pathway performance in mainstream wastewater treatment

Duan

Wang

Erler

et al. 2018

Science of The Total Environment

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“…The key factor underpinning the FNA sludge treatment approach is the fact that AOB are more resilient to the treatment and therefore are less or even not inactivated. Batch tests with FNA treatment consistently suggest selective inactivation of NOB activities against AOB , Jiang et al 2018, Ma et al 2017b, Wang et al 2016a, Wang et al 2014b. The mechanism of the sludge treatment approach can be interpreted as manipulating SRTs of AOB and NOB in the bioreactor.…”

Section: Achieving Energy and Carbon Efficient Nitrogen Removal In Mamentioning

confidence: 93%

Improving wastewater management using free nitrous acid (FNA)

et al. 2020

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Enhancing nitrite production rate made anammox bacteria have a competitive advantage over nitrite oxidizing bacteria in mainstream anammox system

Zhao

Zhang

et al. 2023

Water Environment Research

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Nitrite oxidizing bacteria (NOB) is easy to accumulate in the mainstream anammox process, leading to the decrease of anammox bacterial abundance and deterioration of nitrogen removal. In this study, anammox bacteria was gradually enriched by increasing nitrite production rate under intermittent aeration despite high NOB abundance. With the DO increased from 0.4 to 0.6 mg/L, Nitrosomonas increased from 0.14% to 0.22%, providing more nitrite for anammox bacteria and promoting its enrichment (grew by 77.4%). Adding extra nitrite of 7.14 mg N/(L·h) during the aeration phase to reactor could further increase anammox bacterial abundance by 117.6%, which was higher than the control reactor (40.2%). In contrast, NOB abundance decreased from 1.4 × 1010 to 1.2 × 1010 copies/L. The results suggested that anammox bacteria had a competitive advantage for nitrite over NOB with increasing nitrite production rate. In addition, Thauera and Dechloromonas, which were responsible for reducing nitrate to nitrite, provided additional substrates for anammox bacteria. Overall, this research provides a new idea for mainstream anammox applications. Practitioner Points Inhibiting NOB might be no longer necessary and difficult for mainstream anammox. Anammox bacteria competed for more nitrite with NOB when nitrite production rate increased. Increasing DO from 0.4 to 0.6 mg/L facilitated anammox bacterial growth and nitrogen removal.

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Inactivation and adaptation of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria when exposed to free nitrous acid

Cited by 105 publications

References 14 publications

Effects of free nitrous acid treatment conditions on the nitrite pathway performance in mainstream wastewater treatment

Effects of free nitrous acid treatment conditions on the nitrite pathway performance in mainstream wastewater treatment

Improving wastewater management using free nitrous acid (FNA)

Enhancing nitrite production rate made anammox bacteria have a competitive advantage over nitrite oxidizing bacteria in mainstream anammox system

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