Inactivation and Photorepair of Enteric Pathogenic Microorganisms with Ultraviolet Irradiation

Hu, Xuexiang; Geng, Shujie; Wang, Xiujuan; Hu, Chun

doi:10.1089/ees.2010.0379

Cited by 13 publications

(5 citation statements)

References 14 publications

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“…In the present study, the maximum level of inactivation observed for MRSA in PBS was 3·4 log, on average, and was achieved with a UV‐C dose of 169·4–628·6 mJ cm −2 . Similarly, several reports (Thurston‐Enriquez et al ; Lazarova and Savoye ; Hu et al ; Malayeri et al ) have shown that a UV‐C dose of 55–170 mJ cm −2 is required to achieve a 4‐log inactivation of MS2 bacteriophage, while in the present study MS2 was only inactivated by 2·9 log with a UV‐C dose of 166·7–645·3 mJ cm −2 . In contrast with these previous reports, we dried the bacterial inoculum onto a solid surface prior to exposure to UV‐C light which may have consequently increased the resistance of the micro‐organism to subsequent stressors such as UV radiation.…”

Section: Discussionsupporting

confidence: 83%

Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and norovirus surrogate

Wallace

Ouellette

Jean

2019

Journal of Applied Microbiology

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Aims The current study aimed to assess the potential of a new high dose ultraviolet (UV) disinfection device to inactivate methicillin‐resistant Staphylococcus aureus (MRSA), Clostridium difficile and a norovirus surrogate on handheld mobile devices, and to compare the efficacy of the UV‐C device to hydrogen peroxide disinfection wipes. Methods and Results Suspensions of MRSA, C. difficile spores and a surrogate for norovirus (MS2) were inoculated onto glass or plastic coupons, with or without organic contamination and were exposed to continuous UV‐C light for 15–60 s (165–646 mJ cm−2) in a self‐contained UV‐C chamber or treated with hydrogen peroxide wipes. Increasing the UV‐C dose from 310 to 650 mJ cm−2 did not result in greater levels of inactivation. UV‐C light inactivated all three micro‐organisms, in the absence of organic contamination, by >2·9 log. Treatment of MRSA, C. difficile spores or MS2, in the presence of organic contamination, with UV‐C light (310–646 mJ cm−2) resulted in 2·3–3·7 log reductions. Treatment of MRSA with UV‐C light provided levels of inactivation comparable to treatment with hydrogen peroxide wipes used following the manufacturer’s instructions. Conclusions UV‐C light and hydrogen peroxide wipes had strong antimicrobial activity against MRSA, C. difficile spores and a norovirus surrogate, in the presence or absence of organic contamination. Significance and Impact of the Study Chemical disinfection wipes are widely used in healthcare facilities, but they are not recommended for use on handheld mobile devices which may harbour pathogenic micro‐organisms. The powerful bactericidal, sporicidal and virucidal activity of this high dose UV‐C light device, shows that this technology is a promising alternative to chemical disinfectants, particularly for control of MRSA.

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Section: Discussionsupporting

confidence: 83%

Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and norovirus surrogate

Wallace

Ouellette

Jean

2019

Journal of Applied Microbiology

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“…Their study indicated that neither 1 h of light exposure nor 1 h of dark treatment post-UV treatment significantly influenced the photoreactivation or dark repair ability of S. Typhimurium on shell eggs. However, photoreactivation of S. dysenteriae and S. Typhimurium was detected in a study done byHu et al, (2012). After UV light treatment, contaminated surface water samples were exposed to a fluorescent lamp with an intensity of 0.066 mW/cm 2 at a wavelength of 360 nm for 3 h. They noticed that photoreactivation decreased significantly with higher UV doses.Various factors may affect photoreactivation Lindenauer & Darby (1994).…”

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confidence: 99%

Effectiveness of UV light as a means to reduce Salmonella contamination on tomatoes and food contact surfaces

Lim

Harrison

2016

Food Control

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The effectiveness of ultraviolet light at a wavelength of 254 nm to reduce Salmonella contamination on tomatoes and food contact surfaces was evaluated. Inoculated tomatoes were exposed to UV-C light at doses ranging from 0 to 223.1 mJ/cm 2. All UV treatments significantly reduced Salmonella populations (p<0.05). The effectiveness of UV-C light in reducing Salmonella contamination on different locations on tomato surfaces under various UV doses (0-117.2 mJ/cm 2) was also explored. Results indicated that regardless of the locations, UV treatment was shown to be effective in decreasing Salmonella populations. Subsequent studies evaluated possible photoreactivation or dark repair of injured Salmonella post-UV treatment. Following UV light exposure at doses of 0, 22.3, 44.6, and 89.2 mJ/cm 2 , tomatoes were either exposed to visible light for 0, 3, and 5 h or stored in the dark for the same amount of time. Photoreactivation was not detected, nor was dark repair. UV light was also evaluated for its effectiveness to reduce Salmonella contamination on food contact surfaces (stainless steel, HDPE, waxed cardboard and PVC). Contaminated coupons were exposed to UV-C light at 0, 3.3, and 19.7 mJ/cm 2. Significant differences were observed between coupons treated with UV light and the controls (p<0.05). Coupons exposed for longer time had greater Salmonella population reductions, except for waxed cardboard coupons. Application of UV-C light to reduce Salmonella contamination in tomato handling facilities is feasible.

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“…Photoreactivation of fecal coliforms and E. coli has been reported (Hallmich and Gehr, 2010;Sommer et al, 2000;Tosa and Hirata, 1999;Zimmer and Slawson, 2002). Hu et al (2012) found that S. Typhimurium, Shigella dysenteriae, and E. coli are able to photoreactivate after UV-C treatment, however Kuo et al (1997) did not notice photoreactivation of S. Typhimurium on shell eggs.…”

Section: Uv-c Light Damage Repair Mechanismmentioning

confidence: 95%

Short-Wave Ultraviolet Light Inactivation of Pathogens in Fruit Juices

Baysal¹

2018

Fruit Juices

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Inactivation and Photorepair of Enteric Pathogenic Microorganisms with Ultraviolet Irradiation

Cited by 13 publications

References 14 publications

Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and norovirus surrogate

Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and norovirus surrogate

Effectiveness of UV light as a means to reduce Salmonella contamination on tomatoes and food contact surfaces

Short-Wave Ultraviolet Light Inactivation of Pathogens in Fruit Juices

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