Inactivation, lysis and degradation by-products of Saccharomyces cerevisiae by electrooxidation using DSA

Abstract

The yeast Saccharomyces cerevisiae, a microorganism with cell walls resistant to many types of treatments, was chosen as a model to study electrochemical disinfection process using dimensionally stable anodes (DSA). DSA electrodes with nominal composition of Ti/RuOTiO and Ti/RuOTiOIrO were evaluated in 0.05 mol L NaSO containing yeast. The results showed inactivation about of 100 % of the microorganisms at Ti/RuOTiO by applying 20 and 60 mA cm after 120 min of electrolysis, while a complete inactivation at Ti/RuOIrOTiO electrode was achieved after 180 min at 60 mA cm. When chloride ions were added in the electrolyte solution, 100 % of the yeast was inactivated at 20 mA cm after 120 min of electrolysis, independent of the anode used. In the absence of chloride, the energy consumption (EC) was of 34.80 kWh m, at 20 mA cm by using Ti/RuOTiO anode. Meanwhile, in the presence of chloride, EC was reduced, requiring 30.24 and 30.99 kWh m at 20 mA cm, for Ti/RuOTiO and Ti/RuOIrOTiO electrodes, respectively, The best performance for cell lysis was obtained in the presence of chloride with EC of 88.80 kWh m (Ti/RuOTiO) and 91.85 kWh m (Ti/RuOIrOTiO) to remove, respectively, 92 and 95 % of density yeast. The results clearly showed that yeast, as a model adopted, was efficiently inactivated and lysed by electrolysis disinfection using DSA-type electrodes.

Novel electro-oxidation unit for electro-disinfection of E. coli and some waterborne pathogens during wastewater treatment: batch and continuous experiments

“…At higher bacterial cell densities, it was found that log 6.31 and log 8.29 CFU/mL of E. coli were effectively inactivated after 4 min and 6 min, respectively. The findings align with previous research that shows that low densities of E. coli cells might be easily destroyed in a shorter amount of time following inactivation 23 . In this study, the EC inactivation, chlorination, ozonation, and the Fenton reaction were all found to be successful in eliminating E. coli with a starting density of 10 8 /mL from wastewater in their investigation.…”

Novel electro-oxidation unite for electro-disinfection of E. coli and some waterborne pathogens during wastewater treatment: Batch and continuous experiments

“…4). The ndings align with previous research that shows that low densities of E.coli cells might be easily killed in a shorter amount of time following inactivation 22 . EC inactivation, chlorination, ozonation, and the Fenton reaction were all found to be successful in eliminating E. coli with a starting density of 10 8 /mL from wastewater in their investigation.…”

Recent Advances in Water and Wastewater Electrodisinfection

“…Current density of 6.70 A m À 2 99 % Removal of bacteria [37] Wastewaters treatment plant effluent Anode of RuÀ Ir/Ti oxide and a graphite felt(GF) cathode, both with and area of 60 mm × 60 mm. Current density of 10 mA/cm 2 and an optimal chloride concentration of 250 mg/L 99.99 % disinfection of E. coli [38] Pathogen killing using a microbial fuel Total inactivation of Scharomyces cerevisiae [56] of chlorides, sulphates and carbonates in an aqueous solution can promote a very efficient generation of free chlorine, peroxodisulfate and percarbonates. Furthermore, some other strong oxidants such as hydroxyl radicals, peroxides, and ozone can be also directly produced from water.…”