Inactivation of Adenovirus and Simian Virus 40 Tumorigenicity in Hamsters by Vaccine Processing Methods

Truffelli, G. T.; Timm, E. A.; Beardmore, W. B.; Mclean, I. W.

doi:10.1128/aem.15.3.516-527.1967

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…Progressive inactivation of oncogenic viruses by gamma or ultraviolet (UV) irradiation has been used to determine the survival rate of various viral functions and to clarify the minimal function or target size of the genome compatible with the phenotypic expression of oncogenicity (3,4,6,17,27,28,29). Results of inactivation of Papova viruses in different laboratories agree on the relative rate of inactivation of some functions of SV40 and polyoma such as the induction of T-antigen and cell DNA synthesis and virus replication (6,7,27,35).…”

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confidence: 99%

Analysis of Minimal Functions of Simian Virus 40 II. Enhancement of Oncogenic Transformation in Vitro by UV Irradiation

Seemayer

Defendi

1973

J Virol

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After light UV irradiation (5,000 to 10,000 ergs/mm2) "complete" and "defective" simian virus 40 (SV40) showed an enhancement of oncogenic transformation capacity in Syrian hamster kidney cells in vitro up to 180 and 270% of the controls, respectively. Simultaneously with the enhancement of transformation, an increase in T-antigen induction was observed in CV-1 cells infected with light UV-irradiated SV40; infectivity, however, was correspondingly reduced by 1 log,o. After strong UV irradiation (10,000 to 80,000 ergs/mm2) of "complete" and "defective" SV40, transformation capacity in vitro proved to be the most resistant viral function. It was only slightly reduced in comparison with a 4 to 5 log10 reduction of infectivity. T-antigen induction of SV40 was also equally resistant to strong UV irradiation. We found no evidence of "multiplicity reactivation" involved in the high resistance of transformation capacity of SV40 after UV irradiation. Syrian hamster kidney cells transformed in vitro by UV-irradiated SV40 contained the SV40-specific T-antigen and showed the same morphology and growth characteristics as cells transformed by non-irradiated "complete" or "defective" SV40. They induced malignant tumors after subcutaneous inoculation into Syrian hamsters.

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confidence: 99%

Analysis of Minimal Functions of Simian Virus 40 II. Enhancement of Oncogenic Transformation in Vitro by UV Irradiation

Seemayer

Defendi

1973

J Virol

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“…Moreover, it was found that inactivated vaccines may be contaminated with oncogenic simian virus SV-40. Although a later study found that the tumorigenic properties were highly susceptible to formaldehyde (51,52), the inadequacy of inactivated vaccines urged the development of a live, oral, enteric-coated adenovirus vaccine. Over several decades of use, no evidence has been reported to suggest the oncogenic capacity of the live vaccine strains (20).…”

Section: Inactivated Vaccinementioning

confidence: 99%

Vaccine development for human mastadenovirus

Chen

Tian

2018

J. Thorac. Dis.

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Human mastadenovirus (HAdVs) can cause a broad spectrum of diseases in both children and adults, including acute respiratory infection, gastroenteritis, epidemic keratoconjunctivitis. Populations susceptible to adenovirus infection include children, immunocompromised patients and military recruits. To date, seven species (A-G) including more than 79 genotypes have been characterized, of which HAdV-B3, B4, B7 and the recently reemerged types 14 and 55 often lead to severe pneumonia. The live oral enteric-coated adenovirus type 4 and 7 vaccine, which was approved for use in US military personnel of 17 through 50 years of age, had been shown to be safe and highly effective in numerous clinical trials and by ongoing surveillance of febrile respiratory illness. However, there is currently no vaccine approved for general use in children and adults in any part of the world. This review article will summarize the epidemiological data available for adenovirus and the effectiveness of the adenovirus vaccine in the US military. It will also provide a brief overview of innovative vaccine strategies, animal models for vaccine evaluation, and issues regarding vaccine production.

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Analysis of minimal functions of simian virus 40

Seemayer

Defendi

1974

Archiv f Virusforschung

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Inactivation of Adenovirus and Simian Virus 40 Tumorigenicity in Hamsters by Vaccine Processing Methods

Cited by 7 publications

References 22 publications

Analysis of Minimal Functions of Simian Virus 40 II. Enhancement of Oncogenic Transformation in Vitro by UV Irradiation

Analysis of Minimal Functions of Simian Virus 40 II. Enhancement of Oncogenic Transformation in Vitro by UV Irradiation

Vaccine development for human mastadenovirus

Analysis of minimal functions of simian virus 40

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