Inactivation of alcohol dehydrogenase (ADH) by ferryl derivatives of human hemoglobin

Kowalczyk, Aleksandra; Puchała, Mieczysław; Wesołowska, Katarzyna; Serafin, Eligiusz

doi:10.1016/j.bbapap.2006.11.005

Cited by 11 publications

(9 citation statements)

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“…3c ). Consistent with the previous reports 23 24 25 , the half autoreduction time of ferryl-Hb was 35-fold longer than that of ROS, and the cytotoxicity of ROS could be greatly promoted by oxidizing Hb to cytotoxic ferryl-Hb in I-ARCs mediated PDT. Due to the prolonged lifetime, ferryl-Hb could diffuse a much longer distance in cells than ROS, thus creating a far more persistent oxidative damage, subsequently led to cell death 26 .…”

Section: Resultssupporting

confidence: 91%

“…It indicated that, the bounteous ROS production was essential for ferryl-Hb conversion. As reported, the half-life of ferryl-Hb was prolonged by several orders of magnitude, compared to ROS 23 24 25 . The level of ROS in I-ARCs quickly declined in solution within 5 min after laser irradiation, confirming its short life span, but the level of ferryl-Hb in I-ARCs remained 94.8% under the same conditions ( Fig.…”

Section: Resultssupporting

confidence: 55%

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Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy

Luo

Zheng

Zhao

et al. 2016

Sci Rep

133

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Photodynamic therapy has been increasingly applied in clinical cancer treatments. However, native hypoxic tumoural microenvironment and lacking oxygen supply are the major barriers hindering photodynamic reactions. To solve this problem, we have developed biomimetic artificial red cells by loading complexes of oxygen-carrier (hemoglobin) and photosensitizer (indocyanine green) for boosted photodynamic strategy. Such nanosystem provides a coupling structure with stable self-oxygen supply and acting as an ideal fluorescent/photoacoustic imaging probe, dynamically monitoring the nanoparticle biodistribution and the treatment of PDT. Upon exposure to near-infrared laser, the remote-triggered photosensitizer generates massive cytotoxic reactive oxygen species (ROS) with sufficient oxygen supply. Importantly, hemoglobin is simultaneously oxidized into the more active and resident ferryl-hemoglobin leading to persistent cytotoxicity. ROS and ferryl-hemoglobin synergistically trigger the oxidative damage of xenograft tumour resulting in complete suppression. The artificial red cells with self-monitoring and boosted photodynamic efficacy could serve as a versatile theranostic platform.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 55%

Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy

Luo

Zheng

Zhao

et al. 2016

Sci Rep

133

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“…Ferryl derivatives are formed upon haemoglobin irradiation under air in the reaction of H 2 O 2 with methaemoglobin (Winterbourn 1990, McArthur & Davies 1993. We have demonstrated previously (Kowalczyk et al 2007) that ferryl derivatives of Hb inactivate ADH. Miura et al (1995Miura et al ( , 1997 have also shown that ferryl derivatives of myoglobin inactivates glyceraldehydes-3-phosphate dehydrogenase, alcohol dehydrogenase and creatine kinase, enzymes with 7SH groups at the active sites.…”

Section: Discussionmentioning

confidence: 96%

Inactivation of chosen dehydrogenases by the products of water radiolysis and secondary albumin and haemoglobin radicals

Kowalczyk

Serafin

Puchała³

2008

International Journal of Radiation Biology

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“…In vivo, these reactions are of physiological relevance un-der ischemic conditions (Patel et al, 1996). Ferryl hemoglobin is able to oxidize proteins, nucleic acids and lipids (Kanner & Harel, 1985;Everse et al, 1994;Goldman et al, 1998;Kowalczyk et al, 2007). The globin radical in ferrylHb is very unstable and rapidly decays to a longer-lived radical-free ferryl form (McArthur & Davies, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…The globin radical in ferrylHb is very unstable and rapidly decays to a longer-lived radical-free ferryl form (McArthur & Davies, 1993). FerrylHb undergoes slow acid-catalyzed autoreduction to the ferric (met) form (Tresoriere et al, 2001), but the regenerated metHb is not identical with the initial met state: some hemichrome and dimers of Hb subunits are also formed (Kowalczyk et al, 2007). The reduction of the ferryl form of hemoglobin is facilitated by some reducing compounds, including ascorbate (Giulivi & Davies, 1990), rutin (Grinberg et al, 1994), urate (Everse & Hsia, 1997), melatonin (Tresoriere et al, 2001), trolox (Tresoriere et al, 2001, and epigallocatechin (Jia & Alayash, 2008).…”

Section: Introductionmentioning

confidence: 99%

Flavonoids as reductants of ferryl hemoglobin.

Gębicka¹,

Banasiak²

2009

Acta Biochim Pol

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The ferryl derivatives of hemoglobin are products of the reactions of oxy- and methemoglobin with hydrogen peroxide. Ferryl hemoglobins, either with or without a radical site on the protein moiety, are oxidizing species. Plant polyphenols, flavonoids, have been shown to act as antioxidants in vivo and in vitro. Reactions of met- and oxyhemoglobin with hydrogen peroxide in the presence of catechin, quercetin and rutin were studied. These flavonoids accelerated reduction of ferryl hemoglobin to methemoglobin. The rate constants of the reactions of ferryl hemoglobin with catechin, quercetin and rutin were in the order of 10(2) M(-1) s(-1), i.e. similar to the rate constants of ferryl hemoglobin with intracellular reducing compounds like urate or ascorbate. The beneficial effect of flavonoids against oxidative damage of hemoglobin caused by hydroperoxides, reported in the literature, is probably, at least in part, connected with the ability of flavonoids to scavenge ferryl hemoglobin.

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Inactivation of alcohol dehydrogenase (ADH) by ferryl derivatives of human hemoglobin

Cited by 11 publications

References 40 publications

Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy

Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy

Inactivation of chosen dehydrogenases by the products of water radiolysis and secondary albumin and haemoglobin radicals

Flavonoids as reductants of ferryl hemoglobin.

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