Inactivation of antibiotic-resistant bacteria by chlorine dioxide in soil and shifts in community composition

Wu, Mingsong; Xu, Xinxin

doi:10.1039/c8ra07997h

Cited by 14 publications

(3 citation statements)

References 45 publications

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“…Some of these genes can be antibiotic resistance genes (ARGs) and can remain undestroyed. Finally, these ARGs can be horizontally transferred to other bacteria, resulting in the emergence of new ARB [20] , [21] . However, the development of technologies to control various antibiotic resistance factors related to ARB is often neglected.…”

Section: Introductionmentioning

confidence: 99%

Effective control of antibiotic resistance using a sonication-based combinational treatment and its application to fresh food

Bai

2022

Ultrasonics Sonochemistry

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Section: Introductionmentioning

confidence: 99%

Effective control of antibiotic resistance using a sonication-based combinational treatment and its application to fresh food

Bai

2022

Ultrasonics Sonochemistry

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“…MIP-C 3 N 4 can catalyze the degradation of ARGs to shorter DNA fragments and further to small molecules, eliminating the risk of ARG repair by transformed bacteria in receiving water systems, which is a major limitation for UV disinfection and chlorination. ,, The chain length distribution of resistance plasmid pET-29a(+) (harboring bla NDM‑1 ) (6255 bp) after treatment was determined by nanopore sequencing (Figure a). After treatment by MIP-C 3 N 4 for 10 min, the plasmids were fragmented (7490 fragments) with an average length of 867.5 bp (Table S5).…”

Section: Resultsmentioning

confidence: 99%

Selective Adsorption and Photocatalytic Degradation of Extracellular Antibiotic Resistance Genes by Molecularly-Imprinted Graphitic Carbon Nitride

Yuan

Zhang

et al. 2020

Environ. Sci. Technol.

103

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There is a growing need to mitigate the discharge of extracellular antibiotic resistance genes (ARGs) from municipal wastewater treatment systems. Here, molecularly-imprinted graphitic carbon nitride (MIP-C 3 N 4 ) nanosheets were synthesized for selective photocatalytic degradation of a plasmid-encoded ARG (bla NDM-1 , coding for multidrug resistance New Delhi metallo-β-lactamase-1) in secondary effluent. Molecular imprinting with guanine enhanced ARG adsorption, which improved the utilization of photogenerated oxidizing species to degrade bla NDM-1 rather than being scavenged by background nontarget constituents. Consequently, photocatalytic removal of bla NDM-1 in secondary effluent with MIP-C 3 N 4 (k = 0.111 ± 0.028 min −1 ) was 37 times faster than with bare graphitic carbon nitride (k = 0.003 ± 0.001 min −1 ) under UVA irradiation (365 nm, 3.64 × 10 −6 Einstein/L•s). MIP-C 3 N 4 can efficiently catalyze the fragmentation of bla NDM-1 , which decreased the potential for ARG repair by transformed bacteria. Molecular imprinting also changed the primary degradation pathway; electron holes (h + ) were the predominant oxidizing species responsible for bla NDM-1 removal with MIP-C 3 N 4 versus free radicals (i.e., •OH and O 2 − ) for coated but nonimprinted C 3 N 4 . Overall, MIP-C 3 N 4 efficiently removed bla NDM-1 from secondary effluent, demonstrating the potential for molecular imprinting to enhance the selectivity and efficacy of photocatalytic processes to mitigate dissemination of antibiotic resistance from sewage treatment systems.

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“…Tertiary treatments like effluent disinfection using UV or chlorination do not suppress the release of ARGs in the environment, these genes can still be detected in disinfected effluents (Pazda et al, 2019). Disinfection can inactivate or select for ARB (Destiani and Templeton, 2019;Wu and Xu, 2019;Yuan et al, 2015), while the genes can remain and be released as extracellular DNA (eDNA) by cell lysis. Most studies on AMR did not consider the eDNA.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance genes and mobile genetic elements removal from treated wastewater by sewage-sludge biochar and iron-oxide coated sand

Calderón-Franco

Apoorva

Medema

et al. 2020

Preprint

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Disinfection of treated wastewater in wastewater treatment plants (WWTPs) is used to minimize emission of coliforms, pathogens, and antibiotic resistant bacteria (ARB) in the environment. However, the fate of free-floating extracellular DNA (eDNA) that do carry antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) is overlooked. Water technologies are central to urban and industrial ecology for sanitation and resource recovery. Biochar produced by pyrolysis of sewage sludge and iron-oxide-coated sands recovered as by-product of drinking water treatment were tested as adsorbents to remove ARGs and MGEs from WWTP effluent. DNA adsorption properties and materials applicability were studied in batch and up-flow column systems at bench scale. Breakthrough curves were measured with ultrapure water and treated wastewater at initial DNA concentrations of 0.1-0.5 mg mL-1 and flow rates of 0.1-0.5 mL min-1. Batch tests with treated wastewater indicated that the adsorption profiles of biochar and iron-oxide coated sand followed a Freundlich isotherm, suggesting a multilayer adsorption of nucleic acids. Sewage-sludge biochar exhibited higher DNA adsorption capacity (1 mg g-1) and longer saturation breakthrough times (4 to 10 times) than iron-oxide coated sand (0.2 mg g-1). The removal of a set of representative ARGs and MGEs was measured by qPCR comparing the inlet and outlet of the plug-flow column fed with treated wastewater. ARGs and MGEs present as free-floating eDNA were adsorbed by sewage-sludge biochar at 85% and iron-oxide coated sand at 54%. From the environmental DNA consisting of the free-floating extracellular DNA plus the intracellular DNA of the cells present in the effluent water, 97% (sewage-sludge biochar) and 66% (iron-oxide coated sand) of the tested genes present were removed. Sewage-sludge biochar displayed interesting properties to minimize the spread of antimicrobial resistances to the aquatic environment while strengthening the role of WWTPs as resource recovery factories.Graphical abstractHighlightsSewage-sludge biochar and iron oxide coated sands were tested to adsorb DNA and cells.Biochar removed 97% of genes tested from environmental DNA of unfiltered effluent.85% of ARGs and MGEs of free-floating extracellular DNA were retained by biochar.Biochar is a WWTP by-product that can be re-used for public health sanitation.

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Inactivation of antibiotic-resistant bacteria by chlorine dioxide in soil and shifts in community composition

Abstract: Antibiotic-resistant bacteria are common widespread in soil and the most resistant species is Staphylococcus aureus. Sphingomonas, Arthrobacter and Massilia are sensitive to ClO2. Micromonosporaceae and Thaumarchaeota are more resistant to ClO2.

Cited by 14 publications

References 45 publications

Effective control of antibiotic resistance using a sonication-based combinational treatment and its application to fresh food

Effective control of antibiotic resistance using a sonication-based combinational treatment and its application to fresh food

Selective Adsorption and Photocatalytic Degradation of Extracellular Antibiotic Resistance Genes by Molecularly-Imprinted Graphitic Carbon Nitride

Antibiotic resistance genes and mobile genetic elements removal from treated wastewater by sewage-sludge biochar and iron-oxide coated sand

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