Inactivation of Cronobacter malonaticus cells and inhibition of its biofilm formation exposed to hydrogen peroxide stress

Ye, Yingwang; Zhang, Maofeng; Jiao, Rui Hua; Ling, Na; Zhang, Xiyan; Tong, Lian; Zeng, Haiyang; Zhang, Jumei; Wu, Qingping

doi:10.3168/jds.2017-13463

Cited by 9 publications

(7 citation statements)

References 27 publications

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“…Based on the screening of random mutant library in C. malonaticus, five factors were identified to involve in tolerance to osmotic stress in previous study (Zhang et al 2018): KefA (potassium efflux protein), YqjF (inner membrane protein), peptidylprolyl isomerase, Cys-tRNA(Pro)/CystRNA(Cys) deacylase and oligo galacturonate lyase. We also demonstrated that outer membrane protein W (OmpW) contributed to the osmotic tolerance in C. sakazakii by gene knockout in our previous study (Ye et al 2018).…”

Section: Introductionmentioning

confidence: 51%

“…We also demonstrated that outer membrane protein W (OmpW) contributed to the osmotic tolerance in C. sakazakii by gene knockout in our previous study (Ye et al . 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Using transposon mutagenesis, Alvarez-Ordonez et al (2014) demonstrated that 29 genes were involved in hyperosmotic tolerance in C. sakazakii. Furthermore, we found that OmpW, potassium efflux protein KefA, inner membrane protein YqjF, peptidylprolyl isomerase, cys-tRNA pro /cys-tRNA cys deacylase and oligogalacturonate lyase enhanced tolerance to osmotic stress using screening of random mutant library in Cronobacter(Ye et al 2018;Zhang et al 2018). As a common key factor in Gram-negative and Gram-positive bacteria, LuxS plays crucial roles in resisting environmental stress, forming biofilm and regulating motility and virulence gene expression(Vendeville et al 2005).…”

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confidence: 98%

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Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Ling

Tong

et al. 2023

Int J of Dairy Tech

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Cronobacter malonaticus is a highly hyperosmotic‐resistant pathogen classified as Class A pathogen by the Food and Agriculture Organization of the United Nations (FAO). This study explored the roles of LuxS as a quorum‐sensing transcription factor in C. malonaticus to tolerate hyperosmotic stress, illustrating that LuxS contributed to survival, biofilm formation and reduced cellular injury when grown with 5.5% NaCl stress. Comparative proteomics between C. malonaticus WT and ΔluxS demonstrated that differentially expressed proteins (DEPs) were abundant in Fe‐S cluster proteins and oxidoreductases, suggesting a potential cross‐tolerance mediated by LuxS could contribute to resisting hyperosmotic stress. Herein, these findings will provide valuable information on LuxS‐mediated osmotic tolerance to controlling C. malonaticus contamination.

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Section: Introductionmentioning

confidence: 51%

“…We also demonstrated that outer membrane protein W (OmpW) contributed to the osmotic tolerance in C. sakazakii by gene knockout in our previous study (Ye et al . 2018).…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 98%

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Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Ling

Tong

et al. 2023

Int J of Dairy Tech

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“…2) (Data S2). As an internal control of the method of biofilm formation, we used the bacterium Cronobacter malonaticus 161007/29 (C. malonaticus 161007/29), which is well known to form biofilm on various surfaces (Ye et al, 2018). Overall, the mean OD 570 for C. malonaticus 161007/29 was significantly higher than the mean OD 570 for Ter-positive (Te-R) and Ter-negative cells (control) (**** P < 0.0001) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Response of Escherichia coli minimal ter operon to UVC and auto-aggregation: pilot study

Jánošíková

Pálková

Salát

et al. 2021

PeerJ

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Aim The study of minimal ter operon as a determinant of tellurium resistance (TeR) is important for the purpose of confirming the relationship of these genes to the pathogenicity of microorganisms. The ter operon is widespread among bacterial species and pathogens, implicated also in phage inhibition, oxidative stress and colicin resistance. So far, there is no experimental evidence for the role of the Escherichia coli (E. coli) minimal ter operon in ultraviolet C (UVC) resistance, biofilm formation and auto-aggregation. To identify connection with UVC resistance of the minimal ter operon, matched pairs of Ter-positive and -negative E. coli cells were stressed and differences in survival and whole genome sequence analysis were performed. This study was aimed also to identify differences in phenotype of cells induced by environmental stress. Methods In the current study, a minimal ter operon(terBCDEΔF) originating from the uropathogenic strain E. coli KL53 was used. Clonogenic assay was the method of choice to determine cell reproductive death after treatment with UVC irradiation at certain time intervals. Bacterial suspensions were irradiated with 254 nm UVC-light (germicidal lamp in biological safety cabinet) in vitro. UVC irradiance output was 2.5 mW/cm2 (calculated at the UVC device aperture) and plate-lamp distance of 60 cm. DNA damage analysis was performed using shotgun sequencing on Illumina MiSeq platform. Biofilm formation was measured by a crystal violet retention assay. Auto-aggregation assay was performed according to the Ghane, Babaeekhou & Ketabi (2020). Results A large fraction of Ter-positive E. coli cells survived treatment with 120-s UVC light (300 mJ/cm2) compared to matched Ter-negative cells; ∼5-fold higher resistance of Ter-positive cells to UVC dose (p = 0.0007). Moreover, UVC surviving Ter-positive cells showed smaller mutation rate as Ter-negative cells. The study demonstrated that a 1200-s exposure to UVC (3,000 mJ/cm2) was sufficient for 100% inhibition of growth for all the Ter-positive and -negative E. coli cells. The Ter-positive strain exhibited of 26% higher auto-aggregation activities and was able to inhibit biofilm formation over than Ter- negative strain (**** P < 0.0001). Conclusion Our study shows that Ter-positive cells display lower sensitivity to UVC radiation, corresponding to a presence in minimal ter operon. In addition, our study suggests that also auto-aggregation ability is related to minimal ter operon. The role of the minimal ter operon (terBCDEΔF) in resistance behavior of E. coli under environmental stress is evident.

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“…Hydrogen peroxide (H 2 O 2 ) is a well-studied sanitizer for inactivate foodborne pathogens. In addition, Ye et al (2018) determined the inhibitory effects of H 2 O 2 on C. malonaticus cells and its biofilm formation. However, information about factors involved in oxidative tolerance in C. malonaticus is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus

Zhang

Tong

et al. 2019

Front. Microbiol.

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Cronobacter species are linked with life-treating diseases in neonates and show strong tolerances to environmental stress. However, the information about factors involved in oxidative tolerance in Cronobacter remains elusive. Here, factors involved in oxidative tolerance in C. malonaticus were identified using a transposon mutagenesis. Eight mutants were successfully screened based on a comparison of the growth of strains from mutant library ( n = 215) and wild type (WT) strain under 1.0 mM H 2 O 2 . Mutating sites including thioredoxin 2, glutaredoxin 3, pantothenate kinase, serine/threonine protein kinase, pyruvate kinase, phospholipase A, ferrous iron transport protein A, and alanine racemase 2 were successfully identified by arbitrary PCR and sequencing alignment. Furthermore, the comparison about quantity and structure of biofilms formation among eight mutants and WT was determined using crystal violet staining (CVS), scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM). Results showed that the biofilms of eight mutants significantly decreased within 48 h compared to that of WT, suggesting that mutating genes play important roles in biofilm formation under oxidative stress. The findings provide valuable information for deeply understanding molecular mechanism about oxidative tolerance of C. malonaticus .

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Inactivation of Cronobacter malonaticus cells and inhibition of its biofilm formation exposed to hydrogen peroxide stress

Cited by 9 publications

References 27 publications

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Response of Escherichia coli minimal ter operon to UVC and auto-aggregation: pilot study

Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus

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