Inactivation of Gram-positive biofilms by low-temperature plasma jet at atmospheric pressure

Marchal, Frédéric; Robert, Hervé; Merbahi, Nofel; Fontagné-Faucher, Catherine; Yousfi, Mohammed; Romain, Charles; Eichwald, Olivier; Rondel, Caroline; Gabriel, Bruno

doi:10.1088/0022-3727/45/34/345202

Cited by 47 publications

(33 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In general, reactive plasma species such as excited atoms, molecules, and radicals are considered to directly impinge on the micro‐organisms from the exterior (targeting at the extracellular matrix and cellular outer membrane, etc.) as oxidants, resulting in acute oxidative processes on the bacterial structure including breaking chemical bonds, producing volatile compounds and molecular fragments, and finally leading to biocidal effects . The accumulated intracellular ROS may also perturb the cellular functions and damage the cellular structure from the interior of the bacteria, thus synergistically resulting in bacteria inactivation in combination with direct plasma etching of the bacteria from the exterior.…”

Section: Resultsmentioning

confidence: 99%

Inactivation Effects of Non‐Thermal Atmospheric‐Pressure Helium Plasma Jet on Staphylococcus aureus Biofilms

Shen

Zhang

et al. 2015

Plasma Processes & Polymers

View full text Add to dashboard Cite

The antimicrobial effects and mechanism of helium atmospheric‐pressure plasma jet (APPJ) treatment on Staphylococcus aureus biofilms are evaluated in vitro. The S. aureus biofilms are more resistant to the plasma treatment than adherent bacteria. The APPJ‐treated S. aureus biofilms disclose a depth/layer‐related intra‐bacterial ROS accumulation effect. Plasma exposure may induce bacterial oxidative stress and trigger the production of intracellular reactive oxygen species (ROS) in the biofilms, which possibly contributes to bacteria death in addition to direct etching from the exterior of bacteria. The findings provide insights into the mechanism of biofilm inactivation by plasma reactive species and plasma‐induced intracellular ROS.

show abstract

Section: Resultsmentioning

confidence: 99%

Inactivation Effects of Non‐Thermal Atmospheric‐Pressure Helium Plasma Jet on Staphylococcus aureus Biofilms

Shen

Zhang

et al. 2015

Plasma Processes & Polymers

View full text Add to dashboard Cite

show abstract

“…As emphasized in the introduction, there are many literature works devoted to inactivation or killing of bacteria either under planktonic form or grouped as a biofilm (see e.g., Refs [7][8][9][10][11][12][13]). The effects of the plasma dose (power over the time exposure) and the inactivation agents (UV, radicals, charged particles, etc.)…”

Section: Bacterial Inactivationmentioning

confidence: 99%

Low‐temperature plasmas at atmospheric pressure: toward new pharmaceutical treatments in medicine

Yousfi

Merbahi

Pathak

et al. 2013

Fundamemntal Clinical Pharma

107

View full text Add to dashboard Cite

This article concerns a new field covered by low-temperature plasmas at atmospheric pressure for medical treatments. This is based on the very attractive possibility to tune and design plasmas as possible pharmaceutical products using selectively some active species (charged particles, radicals, atomic and molecular agents, UV radiations) and even electric fields self-generated by the plasma. The delivery of active species occurs at the gaseous level. This means that there is no need for a carrier medium, and the treatment of living tissue or surface is optimal because plasmas can penetrate small pores, spread over rough surfaces, and reach both prokaryotic and eukaryotic cells. The present article gives first a review on the main low-temperature plasma setups potentially usable for medical treatments with an emphasis on the setups as, for instance, plasma jets developed in our laboratory. Then, the present article gives a review of the current state of the art of such plasmas as pharmaceutical products or therapeutic tools in medicine with a light on a selection of forefront researches particularly in the field of chronic wounds, blood coagulation, and cancer treatment.

show abstract

“…And it required a 70-min treatment time for a reduction by 6 log units for biofilm cells. Marchal et al (2012) evaluated the efficiency of a lowtemperature, DC-corona discharge plasma jet on the inactivation of adherent cells and 48-h-old biofilms of Weissella confusa. Biofilms were grown on de Man-Rogosa-Sharpe agar with and without sucrose supplementation.…”

Section: Opportunistic Bacterial Pathogensmentioning

confidence: 99%

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

Puligundla

Mok

2017

J Appl Microbiol

View full text Add to dashboard Cite

Summary Biofilms as complex microbial communities attached to surfaces pose several challenges in different sectors, ranging from food and healthcare to desalination and power generation. The biofilm mode of growth allows microorganisms to survive in hostile environments and biofilm cells exhibit distinct physiology and behaviour in comparison with their planktonic counterparts. They are ubiquitous, resilient and difficult to eradicate due to their resistant phenotype. Several chemical‐based cleaning and disinfection regimens are conventionally used against biofilm‐dwelling micro‐organisms in vitro. Although such approaches are generally considered to be effective, they may contribute to the dissemination of antimicrobial resistance and environmental pollution. Consequently, advanced green technologies for biofilm control are constantly emerging. Disinfection using nonthermal plasmas (NTPs) is one of the novel strategies having a great potential for control of biofilms of a broad spectrum of micro‐organisms. This review discusses several aspects related to the inactivation of biofilm‐associated bacteria and fungi by different types of NTPs under in vitro conditions. A brief introduction summarizes prevailing methods in biofilm inactivation, followed by introduction to gas discharge plasmas, active plasma species and their inactivating mechanism. Subsequently, significance and aspects of NTP inactivation of biofilm‐associated bacteria, especially those of medical importance, including opportunistic pathogens, oral pathogenic bacteria, foodborne pathogens and implant bacteria, are discussed. The remainder of the review discusses majorly about the synergistic effect of NTPs and their activity against biofilm‐associated fungi, especially Candida species.

show abstract

Inactivation of Gram-positive biofilms by low-temperature plasma jet at atmospheric pressure

Cited by 47 publications

References 55 publications

Inactivation Effects of Non‐Thermal Atmospheric‐Pressure Helium Plasma Jet on Staphylococcus aureus Biofilms

Inactivation Effects of Non‐Thermal Atmospheric‐Pressure Helium Plasma Jet on Staphylococcus aureus Biofilms

Low‐temperature plasmas at atmospheric pressure: toward new pharmaceutical treatments in medicine

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

Contact Info

Product

Resources

About