Inactivation of <i>srtA</i> gene of <i>Streptococcus mutans</i> inhibits dextran‐dependent aggregation by glucan‐binding protein C

Igarashi, Takeshi; Asaga, E.; Satō, Yutaka; Goto, Nobuichi

doi:10.1046/j.0902-0055.2003.00104.x

Cited by 19 publications

(20 citation statements)

References 32 publications

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“…The present and previous studies have shown that the S. mutans SrtA is involved in cell wall anchoring of Dex, PAc, and GbpC (this study, 12, 13), implying that the S. mutans mutant lacking SrtA loses biological functions mediated by Dex, PAc, and GbpC molecules on the cell surface of S. mutans . These phenomena appear to occur not only as the result of inactivation of the srtA gene but also due to the inhibition of the SrtA enzyme of S. mutans .…”

Section: Discussionsupporting

confidence: 57%

“…This suggests that the cell wall‐anchored proteins are associated with the pathogenesis of gram‐positive bacteria (3, 14, 16). In a more recent study we determined the complete nucleotide sequence of the srtA gene of S. mutans and demonstrated that SrtA catalyzes anchoring of a surface protein antigen (PAc) and glucan‐binding protein C (GbpC) to the cell wall in S. mutans (12, 13).…”

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confidence: 99%

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Roles of Streptococcus mutans dextranase anchored to the cell wall by sortase

Igarashi

Asaga

Goto

2004

Oral Microbiology and Immunology

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In order to clarify the role that sortase (SrtA) plays in anchoring dextranase (Dex) to the cell wall of Streptococcus mutans, both Dex- and SrtA- mutants were constructed by insertional inactivation of the respective genes. Western blot analysis with a Dex antiserum showed that in the srtA mutant the Dex was not bound to the cell wall but was secreted into the culture supernatant. In contrast, in the wild type, Dex remained cell-wall-associated. Biological properties of the srtA mutant were examined in dextran fermentation, colony morphology and adherence to a smooth surface. The srtA mutant, as well as the wild type, retained the ability to ferment dextran. However, the colony morphology of the srtA mutant on Todd Hewitt agar containing sucrose was much larger than that of the wild type and showed a ring-like structure. In addition, the srtA mutant was more adhesive to a smooth surface than the wild type when sucrose was present. However, the adhesion of the srtA mutant remarkably decreased by addition of exogenous dextranase. These studies suggest that the SrtA mediates Dex-anchoring to the cell wall in S. mutans, and cell wall-anchored Dex plays a role in controlling both the adhesive properties of extracellular glucan and the ability to utilize extracellular glucan as a nutrient source. In contrast, extracellular Dex is only responsible for degrading extracellular glucan as a nutrient source.

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Section: Discussionsupporting

confidence: 57%

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confidence: 99%

Roles of Streptococcus mutans dextranase anchored to the cell wall by sortase

Igarashi

Asaga

Goto

2004

Oral Microbiology and Immunology

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“…Recent studies demonstrated that the S. mutans sortase was involved in the cell wall anchoring of three of them, P1 (11,17), GbpC (13), and DexA (12). These results suggest that S. mutans cells lacking an active sortase may lose the biological function mediated by theses cell surface proteins.…”

mentioning

confidence: 68%

Involvement of Sortase Anchoring of Cell Wall Proteins in Biofilm Formation by Streptococcus mutans

et al. 2005

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Streptococcus mutans is one of the best-known biofilm-forming organisms associated with humans. We investigated the role of the sortase gene (srtA) in monospecies biofilm formation and observed that inactivation of srtA caused a decrease in biofilm formation. Genes encoding three putative sortase-dependent proteins were also found to be up-regulated in biofilms versus planktonic cells and mutations in these genes resulted in reduced biofilm biomass.

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“…This results in the alteration of the solubility and adhesive properties of the glucan substrate and promotes S. mutans biofilm formation (80 (49,158). As may be expected from the lack of anchoring of several adhesins, S. mutans srtA variants displayed a remarkable reduction in their ability to form biofilms (107), in the adhesion to saliva-coated hydroxyapatite in vitro and the colonization of rat teeth in vivo (105), and in the ability to aggregate in the presence of dextrose (82). Interestingly, two S. mutans clinical isolates contain deleterious mutations in the srtA gene.…”

Section: Oral Streptococcimentioning

confidence: 99%

Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria

Marraffini

DeDent²,

Schneewind³

2006

Microbiol Mol Biol Rev

608

600

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SUMMARY The cell wall envelopes of gram-positive bacteria represent a surface organelle that not only functions as a cytoskeletal element but also promotes interactions between bacteria and their environment. Cell wall peptidoglycan is covalently and noncovalently decorated with teichoic acids, polysaccharides, and proteins. The sum of these molecular decorations provides bacterial envelopes with species- and strain-specific properties that are ultimately responsible for bacterial virulence, interactions with host immune systems, and the development of disease symptoms or successful outcomes of infections. Surface proteins typically carry two topogenic sequences, i.e., N-terminal signal peptides and C-terminal sorting signals. Sortases catalyze a transpeptidation reaction by first cleaving a surface protein substrate at the cell wall sorting signal. The resulting acyl enzyme intermediates between sortases and their substrates are then resolved by the nucleophilic attack of amino groups, typically provided by the cell wall cross bridges of peptidoglycan precursors. The surface protein linked to peptidoglycan is then incorporated into the envelope and displayed on the microbial surface. This review focuses on the mechanisms of surface protein anchoring to the cell wall envelope by sortases and the role that these enzymes play in bacterial physiology and pathogenesis.

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Inactivation of srtA gene of Streptococcus mutans inhibits dextran‐dependent aggregation by glucan‐binding protein C

Cited by 19 publications

References 32 publications

Roles of Streptococcus mutans dextranase anchored to the cell wall by sortase

Roles of Streptococcus mutans dextranase anchored to the cell wall by sortase

Involvement of Sortase Anchoring of Cell Wall Proteins in Biofilm Formation by Streptococcus mutans

Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria

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