Inactivation of Lipid‐Enveloped Viruses in Labile Blood Derivatives by Unsaturated Fatty Acids

Horowitz, Bernard; Piët, Marcel P.J.; Prince, Alfred M.; Edwards, Carol A.; Lippin, Arnold; Walakovits, Lori A.

doi:10.1111/j.1423-0410.1988.tb01606.x

Cited by 38 publications

(10 citation statements)

References 36 publications

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“…This could indicate that a potential protective effect of DGLA on progression to severe dengue might be mediated through reduced inflammation. Another intriguing potential mechanism could be related to virucidal activity against encapsulated viruses that some longchain n-6 PUFA have shown in vitro (40)(41)(42)(43) . DGLA breastmilk concentrations were inversely related to cell-free and cellassociated HIV load in milk in a study of Tanzanian HIVinfected women (44) .…”

Section: Discussionmentioning

confidence: 99%

Serum fatty acids and progression from dengue fever to dengue haemorrhagic fever/dengue shock syndrome

et al. 2018

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Polyunsaturated fatty acids (PUFA) might modulate inflammatory responses involved in the development of severe dengue. We aimed to examine whether serum PUFA concentrations in patients diagnosed with dengue fever (DF) were related to the risk of progression to dengue hemorrhagic fever / dengue shock syndrome (DHF/DSS). A secondary aim was to assess correlations between fatty acids (FA) and inflammatory biomarkers in DF patients. We conducted a prospective case-control study nested within a cohort of DF patients who were followed during the acute episode. We compared the distribution of individual FA % of total FA concentration at onset of fever between 109 cases who progressed to DHF/DSS and 235 DF non progressing controls using unconditional logistic regression. We estimated correlations between baseline FA and cytokine concentrations, and compared FA concentrations between the acute episode and >1 y post-convalescence in a subgroup. Docosahexaenoic acid (DHA) was positively related to progression to DHF/DSS (multivariable adjusted odds ratio [AOR] for DHA in quintile 5 vs. 1 = 5.34, 95% confidence interval [CI]: 2.03, 14.1; P, trend = 0.007). Dihomo-γ-linolenic acid (DGLA) was inversely associated with progression (AOR for quintile 5 vs. 1 = 0.30, 95% CI: 0.13, 0.69; P, trend = 0.007). Pentadecanoic acid concentrations were inversely related to DHF/DSS. Correlations of PUFA with cytokines at baseline were low. PUFA were lower during the acute episode than in a disease-free period. In conclusion, serum DHA in DF patients predicts higher odds of progression to DHF/DSS whereas DGLA and pentadecanoic acid predict lower odds.

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Section: Discussionmentioning

confidence: 99%

Serum fatty acids and progression from dengue fever to dengue haemorrhagic fever/dengue shock syndrome

et al. 2018

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“…Fatty acids and fatty acid-containing molecules were found to inactivate enveloped viruses, HSV-1 included, by disintegrating the virus lipid envelope (Horowitz et al, 1988;Kohn et al, 1980;Thormar et al, 1987). On the other hand, Hilmarsson et al (2005) observed that the acidic environment makes HSV more sensitive to certain virucidal lipids (fatty alcohols and lipids), possibly by ionic changes in the envelope proteins.…”

Section: Discussionmentioning

confidence: 99%

Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate

Remichkova

Galabova

Roeva

et al. 2008

Zeitschrift Für Naturforschung C

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The rhamnolipid biosurfactant PS-17 and its complex with the polysaccharide alginate, both produced by the Pseudomonas sp. S-17 strain, were studied for their antiviral activity against herpes simplex virus (HSV) types 1 and 2. They significantly inhibited the herpesvirus cytopathic effect (CPE) in the Madin-Darby bovine kidney (MDBK) cell line. The investigations were carried out according to the CPE inhibition assay protocol. The suppressive effect of the compounds on HSV replication was dose-dependent and occurred at concentrations lower than the critical micelle concentration of the surfactant. The 50% inhibitory concentration (IC 50 ) of rhamnolipid PS-17 was 14.5 μg/ml against HSV-1 and 13 μg/ml against HSV-2. The IC 50 values of the complex were 435 μg/ml for HSV-1 and 482 μg/ml for HSV-2. The inhibitory effects of the substances were confirmed by measuring the infectious virus yields with the multicycle virus growth experimental design as well: Δlog CCID 50 of 1.84Ð2.0 against the two types of herpes simplex viruses by rhamnolipid PS-17 (20 μg/ml), and a strong reduction of the HSV-2 virus yield under the effect of the alginate complex at a concentration of 450 μg/ml. The results indicate that rhamnolipid PS-17 and its alginate complex may be considered as promising substances for the development of anti-herpetic compounds.

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“…HIV inactivation study was performed using a high-titered stock purchased from Bionetics Research Inc. (Kensington, Md., USA). PEG-precipitated samples were suspended in culture medium and added to lymphocytes cultures [39]. Supernatants were checked every 3 days for quantitation of HIV-P24Ag using the Abbott HIV EIA kit.…”

Section: Virus Studymentioning

confidence: 99%

Biochemical and Physical Properties of a Solvent‐Detergent‐Treated Fibrin Glue

Burnouf-Radosevich¹,

Burnouf²,

Huart³

1990

Vox Sanguinis

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A fibrin glue preparation has been obtained from pooled human plasma using a procedure which includes a solvent-detergent (SD) treatment to inactivate lipid-enveloped viruses. The SD treatment inactivated greater than or equal to 5.5 log10 of HIV in less than 45 min, and greater than or equal to 5 log10 and greater than or equal to 6.5 log10 of VSV and Sindbis virus, respectively, in less than 2 h. The product was found to contain high quantities of fibrinogen (116 +/- 2.49 g/l; n = 12), factor XIII (35 +/- 2.88 U/ml) and von Willebrand factor (23 +/- 1.9 U/ml ristocetin cofactor activity), and relatively low levels of fibronectin (5.9 +/- 0.51 g/l). Plasminogen, the precursor of plasmin, which may play a negative role by decreasing the resistance of the fibrin clot, was at only 0.03 g/l. Cellulose acetate electrophoresis showed 95% gamma-proteins and 5% alpha-2-beta proteins. Sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions detected three main protein bands with apparent molecular weights of 65, 56 and 47 kilodaltons, probably corresponding to the alpha, beta, and gamma fibrinogen subunits. Other characteristics of the product included (1) high clottability of fibrinogen (over 85%); (2) absence of low molecular weight fibrin degradation products; (3) rapid solubilization at room temperature (less than 10 min); (4) high tensile strength (202 +/- 27 g/cm2 after 2 h of application), and (5) high elasticity of the fibrin clot. In addition, scanning electron microscopy revealed a highly organized structure showing tridimensional arrangement of the fibrin fibers. SD treated fibrin glue should efficiently replace autologous fibrinogen or cryoprecipitate preparations for surgical application.

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Inactivation of Lipid‐Enveloped Viruses in Labile Blood Derivatives by Unsaturated Fatty Acids

Cited by 38 publications

References 36 publications

Serum fatty acids and progression from dengue fever to dengue haemorrhagic fever/dengue shock syndrome

Serum fatty acids and progression from dengue fever to dengue haemorrhagic fever/dengue shock syndrome

Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate

Biochemical and Physical Properties of a Solvent‐Detergent‐Treated Fibrin Glue

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